This is the first study demonstrating that ortho-phthalic acid esters, dibutylphthalate (DBP) and di-(2-ethylhexyl)-o-phthalate (DEHP), inhibit the ability to form biofilms of the biotrophic pathogen Clavibacter michiganensis ssp. sepedonicus and Pectobacterium carotovorum ssp. carotovorum necrotroph. Inhibition of biofilm formation depends on the DBP and DEHP concentrations.
Rapeseed ( Brassica napus L.) plants transformed with the acb gene encoding acyl-CoA-binding protein from Arabidopsis thaliana were used to demonstrate the physiological effects of transformation as related to the genetic vector structure. The opportunities and limitations of the genetic engineering technologies in the studies of particular proteins are under discussion.
Key words: Brassica napus -genetic vector structure -the physiological effects of transformationAbbreviations : ACBP-acyl-CoA-binding proteins; EA-erucic acid; FA-fatty acid.
Optically active bis-2R(-)ethylhexyl o-phthalate was obtained with 0.18% yield from dry cultured cells of Aconitum baicalense Turcz ex Rapaics 1907 by extraction with petroleum ether followed by silica gel column chromatography. Its structure was confirmed by the analysis of C andH NMR spectra. Seasonal fluctuations of quantitative phthalate content in A. baicalense cells were identified. The tests were performed under conditions excluding the presence of phthalates in reagents, materials, and laboratory dishes. The same substance was shown to be produced by cultivated cells of other plants. Biosynthesis of esters of ortho-phthalic acid by cultivated plant cells was discovered for the first time.
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