Ortho-phthalic acid esters in lipophilic extract from the cell culture of Aconitum baicalense Turcz ex Rapaics 1907

Semenov, A. A.; Enikeev, A. G.; Snetkova, L V; Permyakov, A. V.; Sokolova, N. A.; Дударева, Л. В.

doi:10.1134/s1607672916060120

Cited by 15 publications

(5 citation statements)

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“…Although some Aconitum species are highly toxic because of aconite alkaloid, many species are essential in the formulation of traditional herbal medicine in Asia ( Zhao et al, 2010 ; Semenov et al, 2016 ; Wada et al, 2016 ; Liang et al, 2017 ). The current state of Aconitum phylogenetics lacks molecular information of some species in Eastern Asia, and thus inhibits identification and germplasm utilization of this genus.…”

Section: Introductionmentioning

confidence: 99%

A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus

Kong

Liu

Yao

et al. 2017

PeerJ

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The herbal medicinal genus Aconitum L., belonging to the Ranunculaceae family, represents the earliest diverging lineage within the eudicots. It currently comprises of two subgenera, A. subgenus Lycoctonum and A. subg. Aconitum. The complete chloroplast (cp) genome sequences were characterized in three species: A. angustius, A. finetianum, and A. sinomontanum in subg. Lycoctonum and compared to other Aconitum species to clarify their phylogenetic relationship and provide molecular information for utilization of Aconitum species particularly in Eastern Asia. The length of the chloroplast genome sequences were 156,109 bp in A. angustius, 155,625 bp in A. finetianum and 157,215 bp in A. sinomontanum, with each species possessing 126 genes with 84 protein coding genes (PCGs). While genomic rearrangements were absent, structural variation was detected in the LSC/IR/SSC boundaries. Five pseudogenes were identified, among which Ψrps19 and Ψycf1 were in the LSC/IR/SSC boundaries, Ψrps16 and ΨinfA in the LSC region, and Ψycf15 in the IRb region. The nucleotide variability (Pi) of Aconitum was estimated to be 0.00549, with comparably higher variations in the LSC and SSC than the IR regions. Eight intergenic regions were revealed to be highly variable and a total of 58–62 simple sequence repeats (SSRs) were detected in all three species. More than 80% of SSRs were present in the LSC region. Altogether, 64.41% and 46.81% of SSRs are mononucleotides in subg. Lycoctonum and subg. Aconitum, respectively, while a higher percentage of di-, tri-, tetra-, and penta- SSRs were present in subg. Aconitum. Most species of subg. Aconitum in Eastern Asia were first used for phylogenetic analyses. The availability of the complete cp genome sequences of these species in subg. Lycoctonum will benefit future phylogenetic analyses and aid in germplasm utilization in Aconitum species.

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Section: Introductionmentioning

confidence: 99%

A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus

Kong

Liu

Yao

et al. 2017

PeerJ

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“…At the same time, the measured stable carbon isotopes 13 C/ 12 C ratio in DEHP explains the phenomenon of its high concentration originated from a potential biotic source. It should be noted that (i) DEHP congener is a potential carcinogenic compound [39] and its MPC is high, equal to 8.0 µg/L; (ii) structurally, DEHP is presented by two stereoisomers, the only one of which, namely bis-2R(-)ethylhexyl phthalate, has been isolated from environmental objects so far; for example, the one isolated from the cells of the Aconitum baicalense (Turcz ex Rapaics 1907) culture and from brown sugar [40,41]; (iii) DEHP toxicological studies were conducted using a synthetic racemic product, while the natural DEHP stereoisomer does not have to obligatory possess the same physiological properties [42]; (iv) while obtaining extreme DEHP concentrations, it is necessary to identify the source which could be biogenic or anthropogenic, one or both, before taking any environment protections.…”

Section: Ecological Rick Assessment Of Paes In Surface Watersmentioning

confidence: 99%

Case Study of Diesters of o-Phthalic Acid in Surface Waters with Background Levels of Pollution

Gorshkov,

Grigoryeva,

Bukin

et al. 2023

Toxics

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Lake Baikal was studied as a model for elucidating the general pattern of o-phthalic acid diester (PAE) distributions in surface waters with background pollution levels. The influence of factors including congeners, concentrations, sampling points, seasons, years, and potential sources was considered and the environmental risk for various hydrobionts was established. Priority PAEs in Baikal waters are represented by dimethyl phthalate (DMP), diethyl phthalates (DEP), di-n-butyl phthalate (DnBP) and di-(2-ethylhexyl)phthalate (DEHP). Statistically valuable average concentrations and ranges for DMP, DEP, DnBP, and DEHP were 0.02 (0.01–0.02), 0.07 (0.06–0.09), 0.55 (0.47–0.66), and 0.30 (0.26–0.34) µg/L, respectively. The main factors determining PAE concentrations were the year and season of sampling, whereas sampling points were not among the factors influencing PAE levels. The distribution of PAEs in the water body was characterized by (i) an even distribution of minor hydrophilic DMP and DEP congeners in the whole water body, (ii) a maximum concentration of hydrophobic DnBP and DEHP congeners in the upper and near-bottom layers of the water column, and (iii) a low concentration of hydrophobic congeners in the near-shore area. The main PAE source was found to be the atmospheric transfer of polluted air masses, while the supply of PAEs from coastal sources to the pelagic zone was low. The contribution of biogenic sources to the background level of PAEs in the surface waters of Lake Baikal was established. The ecological risk of the background concentration level of PAEs for Lake Baikal biota was estimated. It was found that (i) DMP and DEP congeners do not represent a risk, or represent a very low risk, (ii) the concentration levels of dominant DnBP and DEHP congeners represent a low risk for crustaceans and fishes but (iii) a rather high risk for algae at a DEHP concentration of 0.30 µg/L.

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“…Measurement of carbon stable isotopes 13 С/ 12 С in DEHP explains a phenomenon of high concentrations of this congener by its income from biotic sources [23]. Due to this fact, we have to notice the following: i) congener DEHP is related to potential carcinogens [37] and MAC for it is "the severest"; ii) DEHP structure is presented by two stereoisomers but only one optically active bis-2R(-)ethylhexyl phthalate is extracted from environmental objects, for example, from a biomass of culturable cells of Aconitum baicalense (Turcz ex Rapaics 1907) and from brown sugar [38,39]; iii) toxicological studies of DEHP were done at a synthetic racemic product, and a natural stereoisomer of DEHP has not to possess mandatory the same physiological properties [40]; iv) while revealing extreme concentrations of congener DEHP, before taking due environment protection measures, it is necessary to identify a DEHP source -biogenic or anthropogenic one.…”

Section: Ecological Rick Assessment Of the Paes In The Surface Watersmentioning

confidence: 99%

Diesters of o-Phthalic Acids in Surface Waters with Background Level of Pollution: Concentrations Levels, Distribution, Sources, and Environmental Risk (Lake Baikal, Russia)

Gorshkov,

Grigoryeva,

Bukin

et al. 2023

Preprint

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We found out main regularities of surface waters pollution by diesters of о-phthalic acid (PAEs) at the background level of concentrations. Lake Baikal was selected as a model for our studies. The composition of PAEs in Baicalins waters is represented by dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DnBP), and di-(2-ethylhexyl) phthalate (DEHP). Their concentrations are assessed in statistically valuable average values and ranges: 0.02 (0.01 – 0.02), 0.07 (0.06 – 0.09), 0.55 (0.47 – 0.66), 0.30 (0.26 – 0.34), µg/L, respectively. Main factors determining PAEs concentration are found out: year and season of sampling. The distribution of PAEs in the water body meets their hydrophobicity and is characterized by: i) even distribution of minor hydrophilic congeners DMP and DEP in the whole water area; ii) maximal concentrations of hydrophobic congeners DnBP and DEHP in the upper and near-bottom layers of the water column; iii) decrease of concentration of hydrophobic congeners in the near-shore zone. The contribution of near-shore sources of PAEs into the pelagic site is minimal, the main pollutants source is atmospheric transfer. A valuable contribution of DEHP from biogenic sources into the background level of PAEs is shown. Within RQ coefficients, we assessed the environmental risk for Baicalins biota.

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Ortho-phthalic acid esters in lipophilic extract from the cell culture of Aconitum baicalense Turcz ex Rapaics 1907

Cited by 15 publications

References 0 publications

A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus

A comparison of chloroplast genome sequences in Aconitum (Ranunculaceae): a traditional herbal medicinal genus

Case Study of Diesters of o-Phthalic Acid in Surface Waters with Background Levels of Pollution

Diesters of o-Phthalic Acids in Surface Waters with Background Level of Pollution: Concentrations Levels, Distribution, Sources, and Environmental Risk (Lake Baikal, Russia)

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