This paper describes a new cell isolation and HLA typing technique, which permits cell separation and HLA class I or class I1 typing to be performed in 70 min. Magnetic monodisperse microspheres (Dynabeads TM) were coated with monoclonal antibodies (MAbs) specific for the CD8 T cell antigen or for HLA class I1 monomorphic epitopes. They could then be used to obtain HLA class I or class I1 positive cells directly from ACD blood in approximately 15 min by the use of magnetic separation. The cells (attached to the microspheres) were subsequently used in microcytotoxic HLA typing (total incubation time of 55 min) using acridin orangelethidiumbromide to stain viable (yellow) and dead (red) cells. It was found that this immunomagnetic (IM) HLA typing technique was specific, has a sensitivity superior to that observed for conventional microcytotoxicity assays and gave low background staining. IM HLA-ABC typing of 50 healthy donors and 10 patients and IM HLA-DR typing of 25 healthy donors and 30 patients gave results corresponding well with that obtained independently by conventional HLA typing (concordancy rates 92-100%). Furthermore, the IM HLA typing technique permitted reliable HLA class I1 typing of blood cells from six patients where conventional HLA class I1 typing was impossible. The IM HLA typing technique also enables HLA class 1 and I1 typing to be quickly and reliably performed on cells from ACD blood of cadaveric donors.
Previous studies have demonstrated a hereditary tendency to develop endometriosis and the incidence of the disease varies in different ethnic groups. The genetic background to these differences has not been clearly explained. The present investigation was performed to seek a possible association between certain HLA antigens and endometriosis. One hundred patients were typed for HLA-A, -B and -C, and of these 24 were also typed for HLA-DR. No significant deviations from the antigen frequencies in a normal population were found. We conclude that the development of endometriosis does not seem to be associated with HLA-A, -B, -C or -DR antigens.
By collecting all the family material available in the seven Danish, Norwegian, and Swedish centres typing for Scandiatransplant, we found 309 families which yielded information on linkage between the first and second segregant series of the HL‐A transplantation system. Information was obtained on 1,362 parental meiotic divisions and recombination has occurred in 11 cases which gives a recombination fraction of 0.0081 with 95% confidence limits of 0.0041–0.0148. The maternal recombination frequency (0.0099) was higher than the paternal one (0.0061), but the difference did not reach statistical significance. Most of the recombinant children belonged to the last half of their sibship.
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