Serological typing of HLA-D: Predictive value in mixed lymphocyte cultures (MLC)

Albrechtsen, D; Bratlie, A.; Nousiainen, Helena; Solheim, Bjarte G.; Winther, Nina; Thorsby, E.

doi:10.1007/bf01563899

Cited by 66 publications

(23 citation statements)

References 13 publications

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“…The severity and distribution of left ventricular hypertrophy varied, but members of generation II were most severely affected. In this generation, two living brothers (II- 11 and II-20) and one brother who died suddenly (II-3) had definite signs of hypertrophic cardiomyopathy with pronounced left ventricular outflow obstruction. They presented clinically with symp- HCM, hypertrophic cardiomyopathy; +, present; 0, absent; NA, data not available; *haplotypes deduced from family typing.…”

Section: Discussionmentioning

confidence: 96%

Hypertrophic cardiomyopathy in three generations of a large Norwegian family. A clinical, echocardiographic, and genetic study.

Haugland¹,

Ohm²,

Boman³

et al. 1986

Heart

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Section: Discussionmentioning

confidence: 96%

Hypertrophic cardiomyopathy in three generations of a large Norwegian family. A clinical, echocardiographic, and genetic study.

Haugland¹,

Ohm²,

Boman³

et al. 1986

Heart

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“…The antigens detected by these antisera, termed D R (D-related), were originally thought to be similar or identical to those responsible for MLC reactivity (Bodmer et al 1978). DR typing of unrelated individuals has also been shown to be predictive of primary MLC reactivity (Albrechtsen et al 1978). However, while the beneficial effect of HLA-DR matching on graft survival has been suggested (for recent references, see Transplant Proc.…”

Section: Discussionmentioning

confidence: 99%

Primed lymphocyte typing predicts MLC reactivity between unrelated individuals

Valentine-Thon

Bach

1983

Tissue Antigens

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The present study was carried out to evaluate the ability of primed lymphocyte typing (PLT) to predict mixed lymphocyte culture (MLC) reactivity between unrelated individuals. Eleven PLT cells generated against independent familial haplotypes, and one PLT cell generated against a homozygous typing cell (HTC), were tested for their response to cells of a random panel of 53 unrelated individuals. From Chi‐square analysis of the response patterns of these 12 cells, nine “PLT specificities” could be defined. Most panel members (81.2%) typed for 1 or 2 of these specificities; equal numbers (9.4%) typed for 3 or none, respectively. Four of these 9 specificities were shown to be significantly correlated with HLA‐Dw antigens defined by HTCs. Typing for these nine PLT specificities was found to be predictive of subsequent primary MLC reactivity between panel members: a) pairs of panel members sharing PLT specificities produced three‐fold lower MLC results on the average than pairs of panel members disparate for PLT specificities (p > 0.0001), and b) in MLC combinations, an increase in number of foreign PLT specificities presented by the stimulator cell was paralleled by a statistically significant increase in MLC reactivity. To the extent that low MLC reactivity is correlated with improved graft survival, the PLT method could have significant value in selecting histocompatible donors for organ transplantation.

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“…Recent research shows that human B lymphocytes possess antigens now called HLA-DRw, which are distinct from HLA-A, -B and -C antigens and which are not present on T lymphocytes. There is a close association between the specificities DRw, as defined by serological methods, and the specificities Dw defined by the MLC (Albrechtsen et al 1978) technique. Preliminary results obtained with a restricted number of sera have shown in MS an increase of two B cell specificities -BT 101 (Compston et al 1976) and group 4 (Terasaki et al 1976).…”

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confidence: 85%

Increased Frequency of HLA—DRw2 and DRw3 in Multiple Sclerosis

Moerloose¹,

Jeannet²,

Martins-da-Silva³

et al. 1979

Tissue Antigens

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Previous studies of multiple sclerosis patients showed the existence of a positive association between multiple sclerosis and HLA—A3 and —B7, as well as a negative association with B12. These observations have been confirmed. In addition, a more marked association has been observed with two recently identified B‐cell antigens, DRw2 and DRw3, closely related to the HLA‐D locus. The presence of cold lymphocytotoxic antibodies was found to bear no relationship with those two specificities. These results suggest that two genes of the HLA—DR region may play a role in the pathogenesis of multiple sclerosis.

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Serological typing of HLA-D: Predictive value in mixed lymphocyte cultures (MLC)

Cited by 66 publications

References 13 publications

Hypertrophic cardiomyopathy in three generations of a large Norwegian family. A clinical, echocardiographic, and genetic study.

Hypertrophic cardiomyopathy in three generations of a large Norwegian family. A clinical, echocardiographic, and genetic study.

Primed lymphocyte typing predicts MLC reactivity between unrelated individuals

Increased Frequency of HLA—DRw2 and DRw3 in Multiple Sclerosis

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