A simple spectrophotometer was designed using cardboard, a DVD, a pocket digital camera, a tripod and a computer. The DVD was used as a diffraction grating and the camera as a light sensor. The spectrophotometer was calibrated using a reference light prior to use. The spectrophotometer was capable of measuring optical wavelengths with a theoretical accuracy as high as 0.2 nm. Using this spectrophotometer, wavelengths are determined via image processing.
Potentiometric sensors had been fabricated by employing the Atrazine MIPs as the working electrode; Aluminum (Al) as the electrode potential. A glass with diameter 3 mm and 6 mm as the vessels for chemical solution. The sensor target was a test solution of Atrazine with different concentration. The potential measured between the electrode contact and reference were increase by the rise of Atrazin concentration. The graph of potential cell versus logarithm of concentration revealed two slopes for Al contact. It implied that the potentiometric sensor was sensitive in the range of concentration 0.44-0.55 mM. Reevaluation of the sensor was conducted 3 months later by following the same procedure. Still, the graph showed a consistent curve that prove sensor stability.
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