グルコースオキシダーゼとカタラーゼ共存下のグルコース酸化速度の解析

Li, Tai-Hsing; Su, Y. Jennifer; Hong, Chia-Hong; Chen, Teh-Liang

doi:10.1252/jcej.27.205

Cited by 10 publications

(3 citation statements)

References 20 publications

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“…1 (Li et al, 1994). In this fermentation, the antifoam ag- accumulated to about 4 g/L when the cell growth was limited by available nutrients.…”

Section: Resultsmentioning

confidence: 90%

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Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Peng¹,

Chen²

1994

Biotechnol Tech

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In addition to acting as an oxygen transfer enhancer, n-hexadecane plays an important role in dominating the mycelial morphology of Penicillium chrysogenum in a submerged penicillin fermentation.The cell morphology is related to the timing of adding nhexadecane to the culture, n-Hexadecane also has a function of suppressing foaming and thus can be employed as an antifoam agent in the fermentation.

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“…1 (Li et al, 1994). In this fermentation, the antifoam ag- accumulated to about 4 g/L when the cell growth was limited by available nutrients.…”

Section: Resultsmentioning

confidence: 90%

“…The effects of the hydrocarbons were attributed to their high oxygen solubilities. However, conclusions on how a fermentation is improved by hydrocarbons are still contradictory (Li et al, 1994). Besides, addition to a culture medium of a hydrocarbon may have influences other than oxygen transfer.…”

Section: Introductionmentioning

confidence: 99%

Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Peng¹,

Chen²

1994

Biotechnol Tech

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“…The occurrence of foaming is facilitated by the existence of surfactants on the air−water interface, in which the surfactants are synthesized by the cells during the fermentation. n ‐Hexadecane can function as an antifoam agent because it can adsorb the surfactants ( 12 − 14), and the efficiency of antifoaming is obviously related to the oil−water interfacial area. Accordingly, the ability of the medium to repress foaming is relatively poor if most n ‐hexadecane is adsorbed by the cloth.…”

Section: Resultsmentioning

confidence: 99%

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

Shen

Chen

et al. 1999

Biotechnology Progress

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Lipase production by Acinetobacter radioresistens was performed in a 2-L tank fermentor equipped with a nonwoven fabric, which was made of nylon 6 fiber and coated with a hydrophobic acrylic resin. The fermentation medium contained 2% (v/v) n-hexadecane as the carbon source. The use of the nonwoven fabric was intended for the dispersion of hydrocarbons; thus, the contact surface for the cells to assimilate n-hexadecane can be increased without using emulsifiers. The formation of lipase was found to be growth-associated when the cells grew on n-hexadecane. The use of the nonwoven fabric increased the lipase yield by 130%. Further supplementation of 0.1% (v/v) olive oil to the medium could markedly shorten the fermentation time, and thus increase the volumetric productivity. The use of the nonwoven fabric offered two additional advantages in the lipase fermentation: ease of foam control and favorable partition of lipase in the aqueous phase.

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Simultaneous covalent immobilization of glucose oxidase and catalase onto chemically modified acrylonitrile copolymer membranes

Godjevargova¹,

Dayal²,

Marinov³

2004

J of Applied Polymer Sci

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Ultrafiltration membranes from acrylonitrile copolymer were chemically modified with different concentrations of hydrogen peroxide (from 5 to 30% H 2 O 2 ). The amount of the amide groups in the modified membranes was determined. The water flow and permeability coefficients of the initial and modified membranes were also researched. The modified membranes were used as carriers for covalent immobilization of the dual enzyme system of glucose oxidase and catalase (GODϩCAT). It was found that the best matrices for immobilization of the dual system were membranes modified with 20% H 2 O 2 and the optimal activity ratio was GOD : CAT ϭ 1 : 5. The glucose conversion efficiency with the dual enzyme system was twice as high as that of bound GOD alone. Some of the basic characteristics (optimum pH, optimum temperature, pH, temperature stability, and storage stability) of the dual enzyme system were determined and compared with characteristics of free and bound enzymes. The catalytic parameters of the enzyme reaction (K m and V max ) were determined with GOD immobilized alone and with the dual system GODϩCAT. The higher rate observed with the dual enzyme system clearly showed the advantage and the efficiency of the immobilized system. Glucose oxidase without catalase was deactivated by H 2 O 2 more rapidly than the immobilized dual GODϩCAT system. These experimental evidences can be explained by the protecting effect of catalase on glucose oxidase from inhibition by H 2 O 2 .

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グルコースオキシダーゼとカタラーゼ共存下のグルコース酸化速度の解析

Cited by 10 publications

References 20 publications

Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

Simultaneous covalent immobilization of glucose oxidase and catalase onto chemically modified acrylonitrile copolymer membranes

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