Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Peng, Chao-Shien; Chen, Teh-Liang

doi:10.1007/bf00152882

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…The morphological character of cells may, in some cases, influence the outcome of microbial reactions. The addition of n-hexadecane to a culture of Penicillium chrysogenum at the beginning of a fermentation has been shown to result in a reduction in penicillin production due to a change in the cell morphology from smooth compact pellets to fluffy loose pellets (Peng and Chen, 1994). Increased yields of biomass and gibberellic acid have been related to differences in morphological character of Fusarium moniliforme grown in fermenters with dissimilar mixing geometries (Priede et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis

O’Shea

Walsh

2000

Biotechnol. Bioeng.

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A semiautomatic image analysis method has been developed to characterize the morphology of the dimorphic yeast Kluyveromyces marxianus var. marxianus (formerly fragilis) NRRLy2415 undergoing alcoholic fermentation of cheese whey permeate. The method is capable of separating cells into six defined categories, varying from simple ovoid yeast cells to branched mycelial cells. A sample size of 300 cells was found to be sufficient to obtain a statistically significant categorization. The processing time for a sample was found to be approximately 90 min. In addition to qualitative characterization, the method permits the measurement of geometric properties such as the width, length, and volume of individual cells or clusters of cells. When the cells analyzed by the automatic method were categorized on a manual basis, the error level in the automatic routine was found to be less than 3%.

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Section: Introductionmentioning

confidence: 99%

Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis

O’Shea

Walsh

2000

Biotechnol. Bioeng.

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“…The occurrence of foaming is facilitated by the existence of surfactants on the air−water interface, in which the surfactants are synthesized by the cells during the fermentation. n ‐Hexadecane can function as an antifoam agent because it can adsorb the surfactants ( 12 − 14), and the efficiency of antifoaming is obviously related to the oil−water interfacial area. Accordingly, the ability of the medium to repress foaming is relatively poor if most n ‐hexadecane is adsorbed by the cloth.…”

Section: Resultsmentioning

confidence: 99%

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

Shen

Chen

et al. 1999

Biotechnology Progress

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Lipase production by Acinetobacter radioresistens was performed in a 2-L tank fermentor equipped with a nonwoven fabric, which was made of nylon 6 fiber and coated with a hydrophobic acrylic resin. The fermentation medium contained 2% (v/v) n-hexadecane as the carbon source. The use of the nonwoven fabric was intended for the dispersion of hydrocarbons; thus, the contact surface for the cells to assimilate n-hexadecane can be increased without using emulsifiers. The formation of lipase was found to be growth-associated when the cells grew on n-hexadecane. The use of the nonwoven fabric increased the lipase yield by 130%. Further supplementation of 0.1% (v/v) olive oil to the medium could markedly shorten the fermentation time, and thus increase the volumetric productivity. The use of the nonwoven fabric offered two additional advantages in the lipase fermentation: ease of foam control and favorable partition of lipase in the aqueous phase.

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The manganese superoxide dismutase from the penicillin producer Penicillium chrysogenum

Dı́ez¹,

Schleissner²,

Moreno³

et al. 1998

Current Genetics

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The antioxidant enzyme superoxide dismutase has been studied in order to define mechanisms for the influence of oxygen on penicillin production. Manganese-containing SOD activity was purified from penicillin-producing cultures of the filamentous fungus Penicillium chrysogenum and reverse genetics was used to identify full-length cDNA and genomic clones. Sequence analysis revealed a 630-bp ORF containing three exons and two introns with fungal consensus splice-site junctions. The deduced amino-acid sequence (210 amino acids; 23.13 kDa) includes conserved residues required for enzymatic activity and metal binding, and shares significant similarity with Mn- and Fe-containing superoxide dismutases. The sod gene is present as a single copy in the genome of different P. chrysogenum strains and its expression level is not correlated with penicillin-G productivity.

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Influence of n-hexadecane on penicillin fermentation with Penicillium chrysogenum: The morphological effect

Cited by 6 publications

References 8 publications

Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis

Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

The manganese superoxide dismutase from the penicillin producer Penicillium chrysogenum

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