Метилирование Промотора Гена Рилина В Периферической Крови И Его Связь С Когнитивным Функционированием Больных Шизофренией

Алфимова, М. В.; Kondratiev, N. V.; Golov, Arkadiy K.; Голимбет, В. Е.

doi:10.1134/s0026898418050026

Cited by 4 publications

(2 citation statements)

References 54 publications

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“…Определение статуса метилирования осуществляли с помощью модифицированного метода SMRT-BS на основе бисульфитной конверсии и высокопроизводительного секвенирования третьего поколения Pacbio [22]. Показатели доли метилирования CpG, расположенных на участках протяженностью около 100 п.н., были усреднены для каждого испытуемого, как описано нами ранее [23], что позволило сократить количество показателей метилирования со 170 до 12. Следует отметить, что уровень метилирования изученных CpG был низким и не различался между больными и здоровыми [23].…”

Section: генетика шизофренииunclassified

“…Показатели доли метилирования CpG, расположенных на участках протяженностью около 100 п.н., были усреднены для каждого испытуемого, как описано нами ранее [23], что позволило сократить количество показателей метилирования со 170 до 12. Следует отметить, что уровень метилирования изученных CpG был низким и не различался между больными и здоровыми [23].…”

Section: генетика шизофренииunclassified

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Relationships of rs7341475 polymorphism and DNA methylation in the reelin gene with schizophrenia symptoms

Алфимова¹,

Kondratyev²,

Golov³

et al. 2018

Z. nevrol. psikhiatr. im. S.S. Korsakova

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Ген RELN, расположенный на хромосомном участке 7q22.1 и кодирующий белок внеклеточного матрикса рилин, рассматривается как один из важных факторов, модифицирующих риск и проявления шизофрении [1, 2]. Это объясняется ролью рилина в развитии нервной системы, в частности в процессах миграции и позиционировании нейронов в эмбриональном периоде. Кроме того, имеются данные о снижении уровня этого гликопротеина в аутопсийных образцах мозга больных шизофренией [1, 2]. При полногеномном анализе данных выявлена ассоциация одно

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Section: генетика шизофренииunclassified

Relationships of rs7341475 polymorphism and DNA methylation in the reelin gene with schizophrenia symptoms

Алфимова¹,

Kondratyev²,

Golov³

et al. 2018

Z. nevrol. psikhiatr. im. S.S. Korsakova

View full text Add to dashboard Cite

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Can epigenetics shine a light on the biological pathways underlying major mental disorders?

et al. 2022

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A significant proportion of the global burden of disease can be attributed to mental illness. Despite important advances in identifying risk factors for mental health conditions, the biological processing underlying causal pathways to disease onset remain poorly understood. This represents a limitation to implement effective prevention and the development of novel pharmacological treatments. Epigenetic mechanisms have emerged as mediators of environmental and genetic risk factors which might play a role in disease onset, including childhood adversity (CA) and cannabis use (CU). Particularly, human research exploring DNA methylation has provided new and promising insights into the role of biological pathways implicated in the aetio-pathogenesis of psychiatric conditions, including: monoaminergic (Serotonin and Dopamine), GABAergic, glutamatergic, neurogenesis, inflammatory and immune response and oxidative stress. While these epigenetic changes have been often studied as disease-specific, similarly to the investigation of environmental risk factors, they are often transdiagnostic. Therefore, we aim to review the existing literature on DNA methylation from human studies of psychiatric diseases (i) to identify epigenetic modifications mapping onto biological pathways either transdiagnostically or specifically related to psychiatric diseases such as Eating Disorders, Post-traumatic Stress Disorder, Bipolar and Psychotic Disorder, Depression, Autism Spectrum Disorder and Anxiety Disorder, and (ii) to investigate a convergence between some of these epigenetic modifications and the exposure to known risk factors for psychiatric disorders such as CA and CU, as well as to other epigenetic confounders in psychiatry research.

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DNA methylation levels of RELN promoter region in ultra-high risk, first episode and chronic schizophrenia cohorts of schizophrenia

et al. 2022

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The essential role of the Reelin gene (RELN) during brain development makes it a prominent candidate in human epigenetic studies of Schizophrenia. Previous literature has reported differing levels of DNA methylation (DNAm) in patients with psychosis. Therefore, this study aimed to (1) examine and compare RELN DNAm levels in subjects at different stages of psychosis cross-sectionally, (2) analyse the effect of antipsychotics (AP) on DNAm, and (3) evaluate the effectiveness and applicability of RELN promoter DNAm as a possible biological-based marker for symptom severity in psychosis.. The study cohort consisted of 56 healthy controls, 87 ultra-high risk (UHR) individuals, 26 first-episode (FE) psychosis individuals and 30 chronic schizophrenia (CS) individuals. The Positive and Negative Syndrome Scale (PANSS) was used to assess Schizophrenia severity. After pyrosequencing selected CpG sites of peripheral blood, the Average mean DNAm levels were compared amongst the 4 subgroups. Our results showed differing levels of DNAm, with UHR having the lowest (7.72 ± 0.19) while the CS had the highest levels (HC: 8.78 ± 0.35; FE: 7.75 ± 0.37; CS: 8.82 ± 0.48). Significantly higher Average mean DNAm levels were found in CS subjects on AP (9.12 ± 0.61) compared to UHR without medication (UHR(−)) (7.39 ± 0.18). A significant association was also observed between the Average mean DNAm of FE and PANSS Negative symptom factor (R2 = 0.237, ß = −0.401, *p = 0.033). In conclusion, our findings suggested different levels of DNAm for subjects at different stages of psychosis. Those subjects that took AP have different DNAm levels. There were significant associations between FE DNAm and Negative PANSS scores. With more future experiments and on larger cohorts, there may be potential use of DNAm of the RELN gene as one of the genes for the biological-based marker for symptom severity in psychosis.

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Метилирование Промотора Гена Рилина В Периферической Крови И Его Связь С Когнитивным Функционированием Больных Шизофренией

Cited by 4 publications

References 54 publications

Relationships of rs7341475 polymorphism and DNA methylation in the reelin gene with schizophrenia symptoms

Relationships of rs7341475 polymorphism and DNA methylation in the reelin gene with schizophrenia symptoms

Can epigenetics shine a light on the biological pathways underlying major mental disorders?

DNA methylation levels of RELN promoter region in ultra-high risk, first episode and chronic schizophrenia cohorts of schizophrenia

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