δEF1 promotes osteolytic metastasis of MDA-MB-231 breast cancer cells by regulating MMP-1 expression

Hu, Fang; Wang, Chuan; Guo, Shaocong; Sun, Wei; Mi, Dong; Gao, Yang; Zhang, Jie; Zhu, Tianhui; Yang, Shuang

doi:10.1016/j.bbagrm.2011.01.003

Cited by 24 publications

(31 citation statements)

References 47 publications

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“…ZEB1, a transcriptional repressor, is hypothesized to have the potential to initiate bone metastasis by inducing osteoclast formation in an MMP-1 dependent manner [62]. ZEB1 expression directly promotes BMP-inhibitor gene transcription and indirectly suppresses BMP-inhibitor reduction via miR-200 family members.…”

Section: Hypoxia Epithelial-to-mesenchymal Transition (Emt) and mentioning

confidence: 99%

“…MMP-2 and MMP-9 degrade type IV collagen, a major component of the basement membrane. Hypoxia induces increased expression and activity of MMP-2 and MMP-9 through a HIF-1-dependent process (Figure 1) [60,61], and increased levels of MMP-2 in breast cancer biopsies were associated with poor prognosis [62]. In addition to secreted MMPs, which are activated by extracellular proteolytic cleavage, hypoxia also induces the membrane bound MT1-MMP in cancer cells by direct binding of HIF-2 to an HRE in the MMP14 gene locus [63].…”

Section: Hif Regulation Of Invasion and Intravasationmentioning

confidence: 99%

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Implications of Hypoxia in Breast Cancer Metastasis to Bone

Gilkes

2016

IJMS

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Most solid tumors contain regions of hypoxia in which increased cell proliferation promotes increased oxygen consumption and the condition is further exacerbated as cancer cells become localized far from a functional blood vessel, further decreasing the oxygen supply. An important mechanism that promotes cell adaptation to hypoxic conditions is the expression of hypoxia-inducible factors (HIFs). Hypoxia-inducible factors transcriptionally regulate many genes involved in the invasion and metastasis of breast cancer cells. Patients, whose primary tumor biopsies show high HIF expression levels, have a greater risk of metastasis. The current review will highlight the potential role of hypoxia in breast cancer metastasis to the bone by considering the regulation of many steps in the metastatic process that include invasion, migration, margination and extravasation, as well as homing signals and regulation of the bone microenvironment.

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Section: Hypoxia Epithelial-to-mesenchymal Transition (Emt) and mentioning

confidence: 99%

Section: Hif Regulation Of Invasion and Intravasationmentioning

confidence: 99%

Implications of Hypoxia in Breast Cancer Metastasis to Bone

Gilkes

2016

IJMS

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“…In the MMP family, MMP-1, MMP-2, and MMP-9 are the most important. Another core step in metastasis is the adhesion to endothelial cells (Kang et al, 2008;Hu et al, 2011). Some bioactive ingredients of phlegm-eliminating herbs have been proven to have a preventive effect on tumor invasion and metastasis.…”

Section: Prevention Of Tumor Invasion and Metastasismentioning

confidence: 99%

Anticancer effects of traditional Chinese herbs with phlegm-eliminating properties – An overview

Xiu

Sun

Jiao

et al. 2015

Journal of Ethnopharmacology

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“…Evidence has suggested that δEF1 is important in breast cancer tumor growth and metastasis (7). To control breast cancer cell proliferation, δEF1 downregulates p21 and concurrently upregulates the expression of CDK2 and CDK4 (8).…”

Section: Introductionmentioning

confidence: 99%

δEF1 upregulates CDK4 transcription via the E2-box element on the CDK4 promoter

Wang

Zhang

et al. 2013

Experimental and Therapeutic Medicine

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The zinc finger-homeodomain transcription factor, δ-crystallin enhancer factor 1 (δEF1) has been identified as a regulatory factor involved in the promotion of breast cancer cell proliferation via the downregulation of p21 and the upregulation of cyclin-dependent kinase-2 (CDK2) and CDK4 expression. However, the molecular mechanisms underlying the regulation of CDK4 expression by δEF1 have not yet been elucidated. The present study demonstrated that the ectopic expression of δEF1 in MDA-MB-231 breast cancer cells significantly increased the activity of the CDK4 promoter. Deletion of the E2-box (CACGTG), which is located at position -197/-191 on the human CDK4 promoter, significantly attenuated the activation of CDK4 transcription by δEF1. In addition, a CDK4 promoter-M construct was generated via site-directed mutagenesis of the E2-box on the human CDK4 promoter. Luciferase assay showed that the activation of CDK4 promoter-M activity by δEF1 was markedly decreased compared with the CDK4-promoter-0.4k promoter. Knockdown of δEF1 using RNA interference resulted in the inhibition of CDK4 promoter activity. These observations suggest that δEF1 upregulates CDK4 transcription via the E2-box element on the CDK4 promoter.

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δEF1 promotes osteolytic metastasis of MDA-MB-231 breast cancer cells by regulating MMP-1 expression

Cited by 24 publications

References 47 publications

Implications of Hypoxia in Breast Cancer Metastasis to Bone

Implications of Hypoxia in Breast Cancer Metastasis to Bone

Anticancer effects of traditional Chinese herbs with phlegm-eliminating properties – An overview

δEF1 upregulates CDK4 transcription via the E2-box element on the CDK4 promoter

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