γ-Tubulin controls neuronal microtubule polarity independently of Golgi outposts

Nguyen, Michelle M.; McCracken, Christie J.; Milner, Elliott S.; Goetschius, Daniel J.; Weiner, Alexis T.; Long, Melissa; Michael, Nick L.; Munro, Sean; Rolls, Melissa M.

doi:10.1091/mbc.e13-09-0515

Cited by 98 publications

(146 citation statements)

References 41 publications

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“…7; Movies 1 and 2). γ-tubulin37C was selected as a control to target with RNAi as it is a γ-tubulin expressed maternally that is not present in somatic tissues including neurons (Wiese, 2008); γ-tubulin23C is the somatic replacement that nucleates microtubules in neurons (Nguyen et al, 2014).…”

Section: Fidgetin Reduction Does Not Alter Microtubule Behavior In Unmentioning

confidence: 99%

The microtubule-severing protein fidgetin acts after dendrite injury to promote their degeneration

Tao

Feng

Rolls

2016

Journal of Cell Science

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After being severed from the cell body, axons initiate an active degeneration program known as Wallerian degeneration. Although dendrites also seem to have an active injury-induced degeneration program, no endogenous regulators of this process are known. Because microtubule disassembly has been proposed to play a role in both pruning and injury-induced degeneration, we used a Drosophila model to identify microtubule regulators involved in dendrite degeneration. We found that, when levels of fidgetin were reduced using mutant or RNA interference (RNAi) strategies, dendrite degeneration was delayed, but axon degeneration and dendrite pruning proceeded with normal timing. We explored two possible ways in which fidgetin could promote dendrite degeneration: (1) by acting constitutively to moderate microtubule stability in dendrites, or (2) by acting specifically after injury to disassemble microtubules. When comparing microtubule dynamics and stability in uninjured neurons with and without fidgetin, we could not find evidence that fidgetin regulated microtubule stability constitutively. However, we identified a fidgetin-dependent increase in microtubule dynamics in severed dendrites. We conclude that fidgetin acts after injury to promote disassembly of microtubules in dendrites severed from the cell body.

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Section: Fidgetin Reduction Does Not Alter Microtubule Behavior In Unmentioning

confidence: 99%

The microtubule-severing protein fidgetin acts after dendrite injury to promote their degeneration

Tao

Feng

Rolls

2016

Journal of Cell Science

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“…52 However, no increase in g-tubulin has been observed at either somatic or dendritic Golgi, 50 and removing Golgi outposts from dendrites using activated kinesin constructs did not disrupt g-tubulin localization. 55 Thus the microtubule nucleation machinery associated with Golgi outposts may not function through the same g-tubulin recruitment mechanism as the PCM. A recent model proposes that Golgi drive microtubule nucleation by localizing the accessory factors that increase the nucleation activity of g-TuRC, while g-TuRC itself remains unenriched in the adjacent cytoplasm.…”

Section: Microtubule Nucleation At Golgi Outpostsmentioning

confidence: 99%

“…55 The direction of polymerization is important during dendrite Figure 2. (A) The centrosome (the structure of interphase centrosome is illustrated 86 ) and (B) somatic Golgi / dendritic Golgi outposts share common molecular machinery for microtubule nucleation.…”

Section: Microtubule Nucleation At Golgi Outpostsmentioning

confidence: 99%

“…57 In class I neurons, however, although the Golgi structural protein GM130 is present in the dendrites, 79 only medial-Golgi markers were detected. 20,55 The transcription factor Dar1 is required to create complex dendrite arbor structures. Dar1 also drives the formation of multi-compartment Golgi outposts; the transcriptional pathway responsible for this has not been determined.…”

Section: Microtubule Nucleation Control For Dendrite Morphogenesismentioning

confidence: 99%

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Microtubule nucleation and organization in dendrites

2016

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“…Unnecessary hospital emergency evacuation is costly, destructive, and unacceptable. Both options of shelter in place and hospital emergency evacuation have the potential of death, injury of patients and personnel, and intensifying treatment conditions of the patients or the injured (25,28,45,47,48).…”

Section: Thematic Analysismentioning

confidence: 99%

Decision-making on Hospital Emergency Evacuation in Disasters and Emergencies: Findings From a Systematic Review

Yaghoubi¹,

Ardalan²,

Zavareh³

et al. 2017

Iran Red Crescent Med J

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Context: Disasters are increasing worldwide, with more devastating effects than ever before. Hospitals must maintain their normal functions or have an evacuation plan due to the rate of damages at the time of a sudden disaster. The present study was conducted to determine the effective determinants and components in hospital evacuation decision-making.Evidence Acquisition: In this systematic review study, which was conducted in 2016, bibliographies, citation databases, and other available records such as international guidelines, documents and reports of organizations and academic dissertations were used to find an answer to the following question: What are the effective components in hospital evacuation decision-making? Finally, 34 articles were included in this systematic review. This systematic review article was checked with PRISMA checklist. Results:The common factors affecting hospital emergency evacuation decision-making were classified into 4 general categories and 40 subcategories, which have been explored during thematic analysis. These 4 categories included hospital infrastructure consequences, threat, internal factors, and external factors. Level of risk was the most important component of threat category and it was mentioned in most of the reviewed literature. Loss of electricity and water, communication and transportation, resources such as staff, and removing patient devices were the most mentioned factors in hospital infrastructure consequences, external factors, and internal factors, respectively. Conclusions:Different variables affect the process of hospital emergency evacuation decision-making. Thus, further studies are needed to develop a decision-making tool for hospital emergency evacuations in Iran.Keywords: Hospital Evacuation, Decision-Making, Disasters, Emergencies ContextThe percentage of disasters and their devastating concerns is increasing worldwide. It was reported that 3.3 million people died in disasters globally from 1970 to 2010 (1, 2). Despite advances in science and technology, it is not yet possible to forecast unexpected events such as earthquakes. In the critical situations following disasters, mortality and morbidity as well as the demand for health care services suddenly increase (3).Health system has a special place among other organizations involved in crisis management because it is the first and foremost need at the time of crises during disasters and unexpected circumstances (4). Hospitals, as key elements in providing preparedness in emergency situations, must remain fully functional at the time of disasters and major incidents (5). They are fundamental resources for successful management of disasters (6).The vulnerability of hospitals in disasters results in instabilities in providing health care (7). Hundreds of hospitals and other health care facilities are destroyed or lose their function in natural disasters worldwide every year Yaghoubi T et al. centers have been evacuated urgently due to fire, incidents related to Hydro-meteorological changes, ter...

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γ-Tubulin controls neuronal microtubule polarity independently of Golgi outposts

Abstract: Microtubule orientation controls polarized trafficking in neurons. In this work, γ-tubulin is identified as a key regulator of both axonal and dendritic microtubule polarity. In addition, the idea that γ-tubulin works in dendrites by residing at Golgi outposts is tested.

Cited by 98 publications

References 41 publications

The microtubule-severing protein fidgetin acts after dendrite injury to promote their degeneration

The microtubule-severing protein fidgetin acts after dendrite injury to promote their degeneration

Microtubule nucleation and organization in dendrites

Decision-making on Hospital Emergency Evacuation in Disasters and Emergencies: Findings From a Systematic Review

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