β-Lactamase production and antibiotic susceptibility pattern of Moraxella catarrhalis isolates collected from two county hospitals in China

Shi, Wei; Wen, Denian; Chen, Chang-Hui; Lin, Yuan; Gao, Wei; Tang, Peng; Cheng, Xiaoping; Yao, Kaihu

doi:10.1186/s12866-018-1217-5

Cited by 16 publications

(18 citation statements)

References 14 publications

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“…Several factors, including age, living conditions, health, environmental agents, and genetics play a role in M. catarrhalis infection (24). In the present study, M. catarrhalis was isolated from 32 (8%) samples, a finding similar to the results of khoramrooz et al(9.5%) of Bro gene from M. catarrhalis, 87%, 73.3%, and 99.4% of the strains possessed Bro gene and were fully resistant against penicillin, ampicillin, and cefalutin (38)(39)(40). Another antimicrobial resistance mechanism in M. catarrhalis is the presence of efflux pump.…”

Section: Discussionsupporting

confidence: 87%

Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis isolated from the respiratory system of patients in northern Iran

2020

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Background and Objectives: Moraxella catarrhalis is considered as an emerging pathogen and a new nosocomial infection agent. This study was conducted to isolate and identify M. catarrhalis from clinical samples (respiratory tracts) and assess them for antimicrobial susceptibility patterns. Methods: In total, 280 samples were collected from patients with respiratory tract infection, and 120 samples were obtained from healthy individuals in the control group. The isolates were identified by phenotyping and genotyping methods, and their antibiotic susceptibility was evaluated using disk diffusion methods. The presence of β-lactamase and efflux pump activity were specified via phenotypic methods. Finally, Bro and acrA genes in the isolates were detected by PCR technique. Results: The frequency of this bacterium was 9.64% (27 out of 280) in patients with respiratory tract infection and 4.16% (5 out of 120) in the control group. Although the isolates were resistant to penicillin, they had various responses against other antibiotics. The results obtained from molecular method showed that 90.6% and 84.3% of the isolates possessed Bro and acrA genes, respectively. There was a significant relationship (P<0.05) between the presence of Bro and acrA genes and antibacterial resistance to ampicillin, amoxicillin, cefazolin, cefuroxime, and chloramphenicol. Conclusion: Our findings confirmed the existence of M. catarrhalis in patients with respiratory diseases and the high prevalence of antibiotic resistant genes in M. catarrhalis isolates. Therefore, timely diagnosis and successful treatment can play important roles in preventing their spread.

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Section: Discussionsupporting

confidence: 87%

Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis isolated from the respiratory system of patients in northern Iran

2020

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“…P. gingivalis itself is often susceptible to β-lactam antibiotics, but recently, the presence of β-lactamase-producing P. gingivalis that degrades β-lactam antibiotics has become a problem [7]. Moreover, in the oral cavity, the β-lactam antibiotics are eventually degraded by β-lactamase-producing bacteria, such as Prevotella intermedia and Moraxella catarrhalis and may not reach P. gingivalis , which is considered to be the reason why the antibacterial treatment is not effective [8,9].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Effect of the Traditional Medicine Hainosan (Painongsan) on Porphyromonas gingivalis

Minami

Takase

Taira³

et al. 2019

Medicines

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Background: Hainosan (Painongsan) is a traditional Japanese and Chinese medicine that is used to treat several purulent diseases, including gingivitis and periodontitis. This formulation contains three crude drug components: The dried immature fruit of Citrus aurantium (Aurantii Fructus Immaturus), the dried root of Paeonia lactiflora (Paeoniae Radix), and the dried root of Platycodon grandiflorum (Platycodi Radix). Here we evaluated the in vitro antibacterial effects of hainosan extract (HNS) and extracts of its three components against Porphyromonas gingivalis, one of the pathogenic bacteria that causes periodontitis. Methods: The antibacterial activities of HNS and its components were examined by counting the number of colony-forming units (CFUs) and through transmission electron microscopy. Results: We found that HNS had direct antibacterial activity against three P. gingivalis isolates (JCM12257, JCM8525, and JCM19600), with HNS-treated cells being significantly smaller than those of untreated bacteria. Extracts of Platycodi Radix and Paeoniae Radix significantly suppressed the growth of P. gingivalis in a dose-dependent manner, with Platycodi Radix extract having the greatest antibacterial effect. In addition, P. gingivalis that were treated with Platycodi Radix extract were significantly larger than those treated with Aurantii Fructus Immaturus or Paeoniae Radix extracts. Further analysis showed that platycodin D, which is one of the ingredients of Platycodi Radix, reduced bacterial growth. Conclusions: Platycodi Radix is the active component in Hainosan and may represent a useful agent for the treatment of P. gingivalis-induced gingivitis and periodontitis.

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“…However, the role of macrolides in PBB remains undefined. Moreover, these pathogens associated with PBB were resistant to macrolides in China, especially Streptococcus pneumoniae with a high resistance rate of over 90% (19,20,27). Therefore, empirical macrolide therapy is not recommended for PBB patients.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and management of protracted bacterial bronchitis: a survey of Chinese pediatricians

Li¹,

Guo²,

Li³

et al. 2021

Ann Transl Med

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Background: Protracted bacterial bronchitis (PBB) is a common cause of chronic wet cough in children.However, it is often misdiagnosed and inappropriately treated in clinical practices. This study aims to investigate the current diagnosis and management of PBB among Chinese pediatricians.Methods: An electronic questionnaire designed to assess the diagnosis and management of PBB was sent to pediatricians in China.Results: A total of 1,022 pediatricians completed the questionnaire. Most (68.8%) of the pediatricians diagnosed PBB in compliance with the guidelines, 44.3% and 24.5% of them followed microbiologybased and clinical-based diagnosis criteria, respectively. Only 40.4% of the pediatricians chose amoxicillinclavulanate as the first-line antibiotic for PBB treatment, 23.7% and 23.5% of them chose third-generation cephalosporins and macrolides, respectively. The majority of pediatricians (75.4%) reported 2-4 weeks of antibiotics course, 19.3% of them prescribed a shorter course and 5% of them selected a longer course.Only 26.3% of the pediatricians performed combined investigations of chest high-resolution computed tomography scan (c-HRCT), bronchoscopy, and immunological tests for recurrences of PBB. Compared with general pediatricians (GP), pediatric pulmonologists (PP) preferred microbiology-based diagnosis criteria, prescribed more amoxicillin-clavulanate, and performed more investigations for recurrent patients (P<0.05). Conclusions:The majority of Chinese pediatricians diagnosed PBB in compliance with guidelines.However, the reasonable antibiotics applications and the investigations for recurrent PBB need to be improved.

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β-Lactamase production and antibiotic susceptibility pattern of Moraxella catarrhalis isolates collected from two county hospitals in China

Cited by 16 publications

References 14 publications

Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis isolated from the respiratory system of patients in northern Iran

Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis isolated from the respiratory system of patients in northern Iran

In Vitro Effect of the Traditional Medicine Hainosan (Painongsan) on Porphyromonas gingivalis

Diagnosis and management of protracted bacterial bronchitis: a survey of Chinese pediatricians

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