β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol

Dokur, Madhavi; Chen, Cui Ping; Advis, J. P.; Sarkar, Dipak K.

doi:10.1016/j.jneuroim.2005.03.015

Cited by 17 publications

(10 citation statements)

References 73 publications

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“…β-Endorphin elevated the levels of IFN-γ and IFN-γ mRNA in rat splenic NK cells [5]. Similar results were previously obtained on a mixed splenocyte culture [4].…”

Section: Resultssupporting

confidence: 87%

β-Endorphin Effects on Antibody Production, Proliferation, and Secretion of Th1/Th2 Cytokines In Vivo

et al. 2012

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Intraperitoneal injection of β-endorphin in doses of 1, 0.01, and 0.0005 μg/kg under conditions of systemic immunization increased the count of antibody-producing cells in the spleen and the titer of anti-erythrocyte antibodies in the plasma of experimental animals. Intraperitoneal β-endorphin stimulated proliferative activity of splenocytes in mice in the presence of both B- and T-cell mitogen, did not change the production of IFN-γ, reduced the level of IL-2, and stimulated the secretion of IL-4, the main Th2-polarizing factor.

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“…β-Endorphin elevated the levels of IFN-γ and IFN-γ mRNA in rat splenic NK cells [5]. Similar results were previously obtained on a mixed splenocyte culture [4].…”

Section: Resultssupporting

confidence: 87%

β-Endorphin Effects on Antibody Production, Proliferation, and Secretion of Th1/Th2 Cytokines In Vivo

et al. 2012

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“…Both acute and chronic ethanol consumption reduces the total number of splenic NK cells and also suppresses splenic NK cell function (Arjona et al, 2004;Boyadjieva et al, 2001;Dokur et al, 2005;Zhou and Meadows, 2003). In this paper, we provide several lines of evidence suggesting that chronic ethanol consumption also markedly suppresses hepatic NK cells.…”

Section: Discussionmentioning

confidence: 74%

“…The immunosuppressive effects of alcohol on splenic NK cells have been extensively investigated and well documented (Arjona et al, 2004;Boyadjieva et al, 2001;Dokur et al, 2005;Zhou and Meadows, 2003). Both acute and chronic ethanol consumption reduces the total number of splenic NK cells and also suppresses splenic NK cell function (Arjona et al, 2004;Boyadjieva et al, 2001;Dokur et al, 2005;Zhou and Meadows, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In mice, it has been shown that ethanol consumption inhibits NK cell activity, antibody-dependent cellular cytotoxicity, lymphocyte proliferation, B-lymphocyte functions, and cytokine production (Cook, 1998;Jerrells, 1991). The effects of ethanol consumption on NK cells in the spleen, and peripheral blood in particular, have been extensively investigated, with findings showing that both acute and chronic ethanol consumption inhibits splenic NK cell function (Arjona et al, 2004;Boyadjieva et al, 2001;Dokur et al, 2005;Zhou and Meadows, 2003). However, the effects of ethanol consumption on hepatic NK cells have received less attention.…”

mentioning

confidence: 99%

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Chronic Ethanol Consumption Inhibits Hepatic Natural Killer Cell Activity and Accelerates Murine Cytomegalovirus‐Induced Hepatitis

Pan

Sun

Jaruga

et al. 2006

Alcoholism Clin & Exp Res

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“…One of the immune components suppressed by prenatal ethanol exposure is the natural killer (NK) cell, which is critical for killing tumor and virally infected cells (Albertsson et al, 2003; Arjona et al, 2006; Colucci et al, 2003; Dewan et al, 2005). NK cells are tightly regulated by the neuroendocrine system involving hypothalamic β -endorphin (BEP) neurons (Boyadjieva et al, 2002, 2006; Dokur et al, 2004a,b, 2005), which are reduced in prenatal ethanol exposed offspring (Arjona et al, 2006). BEP neurons are believed to reduce corticotrophin releasing hormone (CRH) neuronal mediate sympathetic influence on NK cell activity in the spleen (Boyadjieva et al, 2006).…”

mentioning

confidence: 99%

β‐Endorphin Neuronal Cell Transplant Reduces Corticotropin Releasing Hormone Hyperresponse to Lipopolysaccharide and Eliminates Natural Killer Cell Functional Deficiencies in Fetal Alcohol Exposed Rats

Boyadjieva

Ortigüela

Arjona

et al. 2009

Alcoholism Clin & Exp Res

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Background Natural killer (NK) cell dysfunction is associated with hyperresponse of corticotropin releasing hormone (CRH) to immune challenge and with a loss of β-endorphin (BEP) neurons in fetal alcohol exposed animals. Recently, we established a method to differentiate neural stem cells into BEP neurons using cyclic adenosine monophosphate (cAMP)-elevating agents in cultures. Hence, we determined whether in vitro differentiated BEP neurons could be used for reversing the compromised stress response and immune function in fetal alcohol exposed rats. Methods To determine the effect of BEP neuron transplants on NK cell function, we implanted in vitro differentiated BEP neurons into the paraventricular nucleus of pubertal and adult male rats exposed to ethanol or control in utero. The functionality of transplanted BEP neurons was determined by measuring proopiomelanocortin (POMC) gene expression in these cells and their effects on CRH gene expression under basal and after lipopolysaccaride (LPS) challenge. In addition, the effectiveness of BEP neurons in activating NK cell functions is determined by measuring NK cell cytolytic activity and interferon-γ (IFN-γ) production in the spleen and in the peripheral blood mononuclear cell (PBMC) following cell transplantation. Results We showed here that when these in vitro differentiated BEP neurons were transplanted into the hypothalamus, they maintain biological functions by producing POMC and reducing the CRH neuronal response to the LPS challenge. BEP neuronal transplants significantly increased NK cell cytolytic activity in the spleen and in the PBMC and increased plasma levels of IFN-γ in control and fetal alcohol exposed rats. Conclusions These data further establish the BEP neuronal regulatory role in the control of CRH and NK cell cytolytic function and identify a possible novel therapy to treat stress hyper-response and immune deficiency in fetal alcohol exposed subjects.

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β-endorphin modulation of interferon-γ, perforin and granzyme B levels in splenic NK cells: Effects of ethanol

Cited by 17 publications

References 73 publications

β-Endorphin Effects on Antibody Production, Proliferation, and Secretion of Th1/Th2 Cytokines In Vivo

β-Endorphin Effects on Antibody Production, Proliferation, and Secretion of Th1/Th2 Cytokines In Vivo

Chronic Ethanol Consumption Inhibits Hepatic Natural Killer Cell Activity and Accelerates Murine Cytomegalovirus‐Induced Hepatitis

β‐Endorphin Neuronal Cell Transplant Reduces Corticotropin Releasing Hormone Hyperresponse to Lipopolysaccharide and Eliminates Natural Killer Cell Functional Deficiencies in Fetal Alcohol Exposed Rats

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