β-Endorphin Effects on Antibody Production, Proliferation, and Secretion of Th1/Th2 Cytokines In Vivo

Abstract

Intraperitoneal injection of β-endorphin in doses of 1, 0.01, and 0.0005 μg/kg under conditions of systemic immunization increased the count of antibody-producing cells in the spleen and the titer of anti-erythrocyte antibodies in the plasma of experimental animals. Intraperitoneal β-endorphin stimulated proliferative activity of splenocytes in mice in the presence of both B- and T-cell mitogen, did not change the production of IFN-γ, reduced the level of IL-2, and stimulated the secretion of IL-4, the main Th2-polarizing factor.

Effect of endomorphins on humoral immune response, Th1/Th2/Th17 cytokine production and CD4⁺, CD8⁺ lymphocyte apoptosis <i>in vivo</i>

“…Thus, endomorphins in vivo naloxone-dependent stimulate antibody formation and enhance CD8 + lymphocyte apoptosis and IL-17 production in dependently of opioid receptor blockade. Previously, we have shown that in vivo administration of β-endorphin to mice at doses of 1; 0.01 and 0.0005 µg/ kg statistically significantly increased the amount of AFC in the spleen, however, this effect was not reversed by naloxone [5]. In the in vitro system, the addition of endomorphins to splenocytes of mice led to naloxone-independent inhibition of the production of antibodies against sheep erythrocytes, at the same time the effect was leveled by the introduction of monoclonal antibodies to endomorphins [1].…”

Dopamine D&lt;sub&gt;1&lt;/sub&gt;- and D&lt;sub&gt;2&lt;/sub&gt;-Receptors in Immunostimulation under Activation of Mu-Opioid Receptors in Mice with Different Psychoemotional States

“…It is well established that neuromediator/neuromodulator mu-opioidergic system is playing an important role in psychoneuroimmunomodulation. There is increasing evidence that activation of mu-OR by agonists of different origin may produce a wide variety of effects on immune parameters [2,3,[25][26][27]. It has been shown earlier that administration of the most widely used agonist of mu-OR DAGO significantly increased the intensity of the immune response in mice of the CBA strain and Wistar rats with an unchanged psychoemotional state [1,4,9,26].…”

Dariers disease and pregnancy

“…Approximately 5µg/mouse of β-End or acetyl β-End in 50µl doi: 10.7243/2053-5309-1-2 saline was intraperitoneally injected [11] throughout the experimental period (the injection once a day for 20 days), whereas saline was injected into control mice.…”

“…NC/Nga mice were established as an inbred strain from Japanese fancy mice in 1957, and have recently been shown to spontaneously develop AD-like dermatitis with immunoglobulin E hyperproduction under air-uncontrolled, conventional circumstances [10,11]. The plasma level of β-End was found to be increased in mice with a mild stress load [9].…”