β-1,3-glucanase activity of two saprophytic yeasts and possible mode of action as biocontrol agents against postharvest diseases

Castoria, Raffaello; Curtis, Filippo De; Lima, Giuseppe; Cicco, V. De

doi:10.1016/s0925-5214(97)00061-6

Cited by 112 publications

(51 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although several possible mechanisms have been reported (Droby and Chalutz, 1994;Janisiewicz and Korsten, 2002;Massart and Jijakli, 2007), the main mode of action of yeast(-like) biocontrol agents is believed to be competition for nutrients and space (Droby and Chalutz, 1994;Lima et al, 1997;Janisiewicz et al, 2000;Castoria et al, 2001). According to the literature dealing with competition for nutrients and space using different antagonist/pathogen(/host) systems (Castoria et al, 1997(Castoria et al, , 2001Lima et al, 1997;Janisiewicz et al, 2000;Guetsky et al, 2002;Vero et al, 2002): (i) most studies have considered either the in vitro or the in situ approach but not both, (ii) the contribution of the competition for nutrients and competition for space has rarely been considered separately, and (iii) in situ assays have been often performed using the Nutrient Yeast Dextrose Broth medium whose composition is not comparable with that of host tissues. In the present study, competition for nutrients as a mode of action of A. pullulans strain Ach1-1 against P. expansum was first examined in vitro using a system that allows discrimination between competition for nutrients and that for space (Janisiewicz et al, 2000) using increasing concentrations of apple juice.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

View full text Add to dashboard Cite

Aureobasidium pullulans strain Ach1-1 was selected for its effectiveness against blue mold caused by Penicillium expansum on stored apple fruit. The possible involvement of competition for nutrients in the biocontrol activity of this antagonistic strain was investigated both in vitro and in situ. For in vitro assays, the effect of strain Ach1-1 on germination percentages of P. expansum conidia was evaluated after a 24 h incubation period in the presence of increasing apple juice concentrations (0-5%) using a system allowing the physical separation of both agents. In the absence of strain Ach1-1, conidial germination was strongly promoted by apple juice whatever the concentration. However, germination was significantly reduced by the presence of strain Ach1-1 except at the highest juice concentration. For conidia previously inhibited at 0.5% juice, germination after 24 h of incubation was partially recovered in the presence of strain Ach1-1 when fresh juice was added to a final concentration of 5%, and completely restored at both 0.5 and 5% juice concentrations in the absence of strain Ach1-1. For in situ assays, strain Ach1-1 was very protective against P. expansum on postharvest wounded apples. However, the application of high concentrations of exogenous sugars, vitamins and most particularly amino acids, significantly reduced such protection. Time-course analysis of apple amino acids at the wound site revealed that these compounds were more depleted in wounds treated with strain Ach1-1 alone and especially in those treated with both agents (strain Ach1-1 and P. expansum) compared to wounds treated with P. expansum alone or to untreated ones. Exogenous amino acids, applied at high concentrations on apple wounds as a mixture of specific amino acid groups or as individuals, significantly decreased strain Ach1-1 efficacy against P. expansum. The present study provides in vitro and in situ evidence that competition for apple nutrients, most particularly amino acids, may be a main mechanism of the biocontrol activity of A. pullulans strain Ach1-1 against blue mold caused by P. expansum on harvested apple fruit.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

View full text Add to dashboard Cite

show abstract

“…Biocontrol of fungal pathogens by beneficial microorganisms is one promising approach. For example, two biocontrol agents (BCAs), the basidiomycete yeasts Rhodosporidium kratochvilovae LS11 (originally named Rhodotorula glutinis) and Cryptococcus laurentii LS28, were effective in reducing the incidence of blue mold on apples with different mechanisms of action (6)(7)(8). The combination of these BCAs with food-grade additives or low doses of recently developed fungicides resulted in a significant rot reduction and in lower patulin contamination in apples (9,10).…”

mentioning

confidence: 99%

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

Ianiri

Idnurm

Wright

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

f Patulin is a mycotoxin that contaminates pome fruits and derived products worldwide. Basidiomycete yeasts belonging to the subphylum Pucciniomycotina have been identified to have the ability to degrade this molecule efficiently and have been explored through different approaches to understand this degradation process. In this study, Sporobolomyces sp. strain IAM 13481 was found to be able to degrade patulin to form two different breakdown products, desoxypatulinic acid and (Z)-ascladiol. To gain insight into the genetic basis of tolerance and degradation of patulin, more than 3,000 transfer DNA (T-DNA) insertional mutants were generated in strain IAM 13481 and screened for the inability to degrade patulin using a bioassay based on the sensitivity of Escherichia coli to patulin. Thirteen mutants showing reduced growth in the presence of patulin were isolated and further characterized. Genes disrupted in patulin-sensitive mutants included homologs of Saccharomyces cerevisiae YCK2, PAC2, DAL5, and VPS8. The patulin-sensitive mutants also exhibited hypersensitivity to reactive oxygen species as well as genotoxic and cell wall-destabilizing agents, suggesting that the inactivated genes are essential for tolerating and overcoming the initial toxicity of patulin. These results support a model whereby patulin degradation occurs through a multistep process that includes an initial tolerance to patulin that utilizes processes common to other external stresses, followed by two separate pathways for degradation.

show abstract

“…Embora os mecanismos de ação dos antagonistas aqui avaliados não tenham sido determinados na presente pesquisa, supõe-se que o sucesso de R. glutinis no controle da antracnose do pimentão pode ser devido: a capacidade da levedura em produzir enzimas como as pectinases, quitinases e glucanases (SARAVANAKUMAR et al 2009;BAUERMAISTER et al 2010), responsáveis pela despolimerização da parede celular de determinados fungos fitopatogênicos, bem como pela competição por nutrientes (CASTORIA et al , 1997), uma vez que as leveduras são hábeis, principalmente, na colonização e competição por espaços e nutrientes na superfície de frutos e folhas (MCLAUGHLIN et al,1990;FILONOW, 1998).…”

Section: França G S Et Alunclassified

Controle pós-colheita da antracnose do pimentão pela levedura Rhodotorula glutinis

et al. 2015

View full text Add to dashboard Cite

RESUMO:Considerando as perdas causadas em pós-colheita pela antracnose no pimentão e à ineficácia das medidas de controle atualmente utilizadas, este trabalho teve por objetivo estudar a potencialidade antagônica in vitro e in vivo de quinze isolados de leveduras à Colletotrichum sp., agente causal da antracnose em pimentão. Foi calculada a porcentagem de inibição do crescimento do fungo dos tratamentos em relação à testemunha nos testes in vitro e determinado o tamanho da área lesionada em frutos através da mensuração do comprimento da lesão em dois sentidos diametricamente opostos nos testes in vivo. As leveduras que obtiveram o melhor resultado no controle da antracnose foram identificadas através de características macroscópicas, microscópicas, fisiológicas, bioquímicas e por taxonomia molecular, sendo os isolados de levedura 13E e 13A1, os que obtiveram as melhores respostas no controle do fitopatógeno tanto in vitro quanto in vivo, identificados como pertencentes à espécie Rhodotorula glutinis.

show abstract

β-1,3-glucanase activity of two saprophytic yeasts and possible mode of action as biocontrol agents against postharvest diseases

Cited by 112 publications

References 23 publications

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

Controle pós-colheita da antracnose do pimentão pela levedura Rhodotorula glutinis

Contact Info

Product

Resources

About