Controle pós-colheita da antracnose do pimentão pela levedura Rhodotorula glutinis

França, Gisely Santana de; Costa-Carvalho, R.R.; Neves, Rejane Pereira; Araújo, Emmanuelle Rodrigues; Laranjeira, Delson

doi:10.14393/bj-v31n2a2015-22387

Cited by 6 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fungus C. gloeosporioides is the main responsible for anthracnose in fruits of Capsicum in Brazil, and can appear in any part of the plant, from its development in the field to even in fruits stored in the post-harvest phase [26]. Colletotrichum species can infect pepper fruits at any stage of maturation.…”

Section: Resultsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

View full text Add to dashboard Cite

There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

View full text Add to dashboard Cite

show abstract

“…C.V (coefficient of variation); Means followed by different letters in the column, differ by Tukey's test (p < 0.05). França et al (2015) evaluating the post-harvest control of anthracnose in peppers by the use of R. glutinis observed that this was more effective in controlling the disease when using yeast in post-harvest fruits, assuming that the success of this yeast in the anthracnose control in peppers may be due to the yeast's ability to produce enzymes such as pectinases, chitins and glucanases (Saravanakumar et al, 2009;Bauermaister et al, 2010), responsible for depolymerizing the cell wall of certain fungi, as well as competition for nutrients. According to Silva et al (2014), among the treatments based on use of antagonists, R. glutinis also presented a satisfactory result in the control of the sweet rot of pepper.…”

Section: Production Of Volatile and Non-volatile Compounds By Yeast And Conidia Germinationmentioning

confidence: 99%

“…The other yeasts do not differ significantly from the control. França et al (2015), found that the most efficient responses in the control of Colletotrichum gloeosporioides in pepper culture were from isolates belonging to the species R. glutinis, with growth inhibition up to 47.99%, being the control mechanisms competition for space and nutrients, production of antibiotics and also for parasitism.…”

Section: Production Of Volatile and Non-volatile Compounds By Yeast And Conidia Germinationmentioning

confidence: 99%

Yeast Potential for the Biological Control of Colletotrichum musae

Rodrigues¹,

Barabasz²,

Silva³

et al. 2020

JAS

View full text Add to dashboard Cite

One of the factors that cause the greatest loss of fruit in post-harvest are diseases, especially rotting such as anthracnose. Therefore, this work aimed to test the potential of the yeasts Candida albicans, Pichia guilliermondii, Rhodotorula glutinis, Saccharomyces cerevisiae, Cryptococcus laurentii and Zygoascus hellenicus in the control of Colletotrichum musae in bananas in post-harvest period. To test the potential of these yeasts, the effect of volatile and non-volatile compounds, culture pairing and spore germination of the fungus C. musae in vitro was evaluated. In post-harvest fruits, the area below the mycelial growth curve (AACCM) and the area below the disease progress curve (AACPD) were evaluated. The yeasts C. albicans, R. glutinis, S. cerevisiae and P. guilliermondii produced volatile compounds with antifungal action, reducing the development of the fungus in vitro. The yeast R. glutinis was shown to be more efficient in reducing mycelial growth in vitro of the fungus through the production of non-volatile compounds. The yeasts C. albicans and P. guilliermondii showed the presence of an inhibition halo. All yeasts induced the germination of C. musae conidia and were not efficient in controlling anthracnose in vivo.

show abstract

“…Nessa perspectiva a antracnose, doença causada por fungos do gênero Colletotrichumé uma das doenças de maior importância para as solanáceas como o pimentão (Capsicum annum) [de França et al 2015]. A antracnose pode atingir todos os estágios de desenvolvimento da planta, desde mudas no viveiro até os frutos após a colheita [de Azevedo et al 2006].…”

Section: Introductionunclassified

Sistema de Classificação do Capsicum Annuum Acometido Pela Doença Fúngica Antracnose Através do Random Forest

Sousa¹,

Brito²

2020

Anais Da Escola Regional De Computação Ceará, Maranhão, Piauí (ERCEMAPI 2020)

View full text Add to dashboard Cite

O uso de técnicas computacionais de Inteligência Artificial tem ganhado espaço no auxílio de diversas problemáticas. Com base nisso foi realizado um estudo visando a utilização do classificador Random Forest, presente no pacote do software WEKA, como ferramenta de auxílio no pré-diagnóstico da doença fúngica antracnose em uma horta de pimentões. A metodologia foi composta a partir de um banco de dados, coletados na cidade de Tianguá-CE, na localidade conhecida como Sitio Córrego. Foram feitos 10 treinamentos com os dados normalizados e balanceados, utilizando a validação cruzada kfold. Os resultados foram analisados estatisticamente obtendo-se uma medida de acerto de 90,84%.

show abstract

Controle pós-colheita da antracnose do pimentão pela levedura Rhodotorula glutinis

Cited by 6 publications

References 31 publications

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Yeast Potential for the Biological Control of Colletotrichum musae

Sistema de Classificação do Capsicum Annuum Acometido Pela Doença Fúngica Antracnose Através do Random Forest

Contact Info

Product

Resources

About