In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun, Sanae Krimi; Bajji, Mohammed; Massart, Sébastien; Labhilili, Mustapha; Jaafari, Samir El; Jijakli, M. Haïssam

doi:10.1016/j.postharvbio.2007.05.005

Cited by 127 publications

(76 citation statements)

References 21 publications

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“…It can be seen that competition of space and nutrition between S. pararoseus Y16 and pathogen is an important reason for controlling postharvest blue mold decay of apples. Similar results for other antagonist yeasts have been reported by [20,21] who described this phenomenon as an advantage to microbial antagonists in competing for nutrients and space, which plays a major role in its biocontrol efficacy.…”

Section: Discussionsupporting

confidence: 88%

Effects of Sporidiobolus pararoseus Y16 on Postharvest Blue Mold Decay and the Defense Response of Apples

Zhao

Sun

Yang

et al. 2018

Journal of Food Quality

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The efficacy of Sporidiobolus pararoseus Y16 in controlling postharvest blue mold caused by Penicillium expansum on apples and the defense response involved were evaluated. The results suggested that the decay incidence of blue mold of apples treated by S. pararoseus Y16 was significantly reduced compared with the control. In vitro testing indicated that germination of spores and germ tube length of P. expansum were markedly inhibited by S. pararoseus Y16. Meanwhile, polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL), and catalase (CAT) activities and several pathogenesis-related (PR) gene expression levels (including PR3, PR4, PR5, and PR9) were determined. In apples, the activities of PPO, POD, CAT, and PAL were significantly induced by S. pararoseus Y16 treatment compared with the control fruits. The relative expression levels of PR3 and PR4 were significantly induced at 4 and 6 d, while PR5 was significantly induced at 4 and 6 d and PR9 was significantly induced at 4 d. Therefore, the reduction in apple fruit decay by S. pararoseus Y16 treatment could be related to the increased activities of related enzymes and proteins involved in the defense against pathogens, which suggest that S. pararoseus Y16 is a potential antagonistic yeast.

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Section: Discussionsupporting

confidence: 88%

Effects of Sporidiobolus pararoseus Y16 on Postharvest Blue Mold Decay and the Defense Response of Apples

Zhao

Sun

Yang

et al. 2018

Journal of Food Quality

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“…Among the other three preparations, the unwashed cell culture mixture and cell-free culture filtrate inhibited the lesion expansion of pear fruits at different levels, and the unwashed cell culture mixture had the better activity. This finding indicates that the competition of R. mucilaginosa for nutrients and space on pear fruits was weakened when the yeast was in a suspension full of nutrients (Zhang et al, 2003;Bencheqroun et al, 2007). It also demonstrates that competition for nutrients in fruit wounds is an important mechanism of yeast antagonism.…”

Section: Discussionmentioning

confidence: 87%

Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits

Yang

et al. 2015

J. Zhejiang Univ. Sci. B

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A total of 20 strains of yeast isolated from Tibetan fermented products were screened for antagonism against blue mold of pear caused by Penicillium expansum. Six isolates that inhibited incidence of postharvest decay by 35% or more were selected for further screening. Among them, the most effective was Rhodotorula mucilaginosa. The results showed that washed cell suspensions of R. mucilaginosa yielded better antagonistic efficacy than unwashed cell-culture mixtures, cell-free culture filtrates, and autoclaved cell cultures. Biocontrol activity improved with increasing concentrations of incubated cells. The best concentration was 1×10 8 cells/ml, at which the incidence of decay was only 16.7% after 6 d of incubation. The germination of conidia of P. expansum in vitro was significantly inhibited by both washed cell-suspensions and unwashed cell-culture mixtures. Rapid colonization by yeast at different concentrations showed a relationship between yeast-cell concentration and biocontrol activity. Although the titratable acidity of pear fruits increased after treatment, R. mucilaginosa did not affect the total soluble solids or ascorbic acid content. This is the first study to report that the yeast R. mucilaginosa from Tibet Autonomous Region of China may have potential as an antagonist to control the postharvest decay of pear fruits.

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“…The ability of strain Ach1-1 to suppress P. expansum development was repeatedly observed both in vitro and in situ (Krimi Bencheqroun et al, 2006). Competition for apple nutrients would be one of the main mechanisms underlying its biocontrol activity on stored apple fruit, amino acids being the most limited nutrients (Krimi Bencheqroun et al, 2007). Among these amino acids, glycine, glutamic acid and especially serine appear to be the most limited nutrients in this mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…Several other strains have been considered as potential biocontrol agents against the main postharvest pathogens (e.g. Botrytis cinerea and Penicillium expansum) on several important crops, including apple (Leibinger et al, 1997;Ippolito et al, 2000;Castoria et al, 2001;Achbani et al, 2005;Krimi Bencheqroun et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…doi: 10.1016/j.postharvbio.2008.05.011 In this context, A. pullulans strains Ach 1-1 and 1113-5 were isolated from 'Golden Delicious' apples for their high antagonistic activity (more than 80%) against B. cinerea and P. expansum, two major postharvest pathogens of apples (Achbani et al, 2005). With regard to strain Ach 1-1, a process suitable for producing large quantities has been developed (Mounir et al, 2007) and competition for apple nutrients, most particularly amino acids, may be the main mechanism of its biocontrol activity against blue mold caused by P. expansum on harvested apple fruit (Krimi Bencheqroun et al, 2007). The practical application of Ach 1-1 and 1113-5 strains as biocontrol agents will require the development of monitoring methods allowing their specific identification and their quantification.…”

Section: Introductionmentioning

confidence: 99%

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Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

Hamouchi

Bajji

Friel

et al. 2008

Postharvest Biology and Technology

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a b s t r a c tAureobasidium pullulans strains Ach 1-1 and 1113-5 are two effective biocontrol agents against Botrytis cinerea and Penicillium expansum on stored apples. In the present work, a monitoring system allowing their identification and quantification was developed. The methodology used consisted of the development of both molecular markers and a semi-selective medium. The random amplified polymorphic DNA (RAPD) technique was applied to a collection of 15 strains of A. pullulans, including Ach 1-1 and 1113-5. Five specific RAPD fragments were amplified for strain Ach 1-1 and three others for strain 1113-5. Among them, a fragment of 528 bp specific to strain Ach 1-1 (generated with the OPR-13 RAPD primer) and another one of 431 bp specific to strain 1113-5 (amplified with the OPQ-03 RAPD primer) were selected, cloned, sequenced, and used to design sequence-characterized amplified region (SCAR) primers. Three different SCAR markers were amplified: two specific to strain Ach 1-1 (189 bp and 387 bp) and one specific to strain 1113-5 (431 bp). These SCAR primers can clearly identify strains Ach 1-1 and 1113-5 among 14 strains of A. pullulans and among eight yeast strains commonly present on apple fruit surfaces. Their selectivity was also tested using DNA extracted from epiphytic microflora of the apple surface. As a semi-selective medium, PDA medium supplemented with 0.5 mg L −1 euparen, 1 mg L −1 sumico, 2.5 mg L −1 hygromycin B, 30 mg L −1 streptomycin sulphate, and 1 mg L −1 cycloheximide was selected. It inhibited the development of the air microflora and appeared highly toxic for the epiphytic microflora of apple surface without altering the growth of the targeted strains Ach 1-1 and 1113-5. The combination of the semi-selective medium and SCAR markers provides a valuable monitoring tool to specifically identify and quantify A. pullulans strain Ach 1-1 and strain 1113-5 and could be used in future studies to evaluate their population dynamics under various laboratory and practical conditions.

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In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Cited by 127 publications

References 21 publications

Effects of Sporidiobolus pararoseus Y16 on Postharvest Blue Mold Decay and the Defense Response of Apples

Effects of Sporidiobolus pararoseus Y16 on Postharvest Blue Mold Decay and the Defense Response of Apples

Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits

Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

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