The Schiff bases of tyrosine and tryptophan (I) 2-((2-hydroxy-3,5-diiodobenzylidene)amino)-3-(1H-indol-3-yl)propanoic acid, (II) 5-(((1-carboxy-2-(1H-indol-3-yl)ethyl)imino)methyl)-2-hydroxybenzoic acid, (III) 2-((2-hydroxy-3,5-diiodobenzylidene)amino)-3-(4hydroxyphenyl)propanoic acid and (IV) 5-(((1-carboxy-2-(4-hydroxyphenyl)ethyl)imino)methyl)-2-hydroxybenzoic acidwere prepared and characterized by physical and analytical data, FTIR , 1 H NMR, 13 C NMR spectra and were screened against gram positive bacteria Staphylococcus aureus, Bacillus subtilis and gram negative bacteria Escherichia coli, Klebsiella aerogenes for antibacterial activity and were screened against Aspergillus niger and Candida albicans for antifungal activity by disc diffusion method. Ciprofloxacin and Nystatin were used as standard for bacteria and fungi.