Zum Mechanismus der Stimulierung der humoralen Antikörperbildung durch Polyanionen

Abstract

The mechanism of the Stimulation of humoral antibody formation by polyanionsThe effect of the polyanion polyacrylic acid on humoral antibody synthesis was studied in mice -immunised with sheep erythrocytes. The synthesis of antibodies against sheep erythrocytes was determined by counting antibody-forming cells in the spleen, and by titration of the antibody circulating in the serum. With low doses of antigen, which alone induce no significant response, the presence of polyacrylic acid resulted in a nearly optimal response, giving values 100 to 260 times higher than the control. With suboptimal doses of antigen, which elicit an easily measurable primary response, the immuno-response is also significantly increased (3-to 4-fold) by the polyanion. At optimal doses of antigen, the polyanion has no stimulatory effect. The same order of stimulation of the immuno-response by the polyanion at low doses of antigen is demonstrable in thymectomised mice. Possible mechanisms for the induction or stimulation of the immuno-response by polyanions are discussed. The possible significance of these mechanisms in elucidating the role and function of the different cooperative cells necessary for the formation of antibodies is discussed.

Stimulation of humoral antibody formation by polyanions. IV. The effects of dextran sulfate on the kinetics of secondary immune response in mice

“…Direct (19 S) and indirect (7 S) PFC in the spleen of the mice were determined a t various times after the first and after the second antigen inoculation, as mentioned in section 3 and as described previously [3]. For statistical analysis the Wilcoxon test was used [ 61.…”

Enhancement of Primary Antigen-Specific Resistance in Infant Mice with Divinyl Ether-Maleic Anhydride

“…In most studies of polyanion adjuvanting effects, sheep erythrocytes have been used as antigens; this system allows for detailed analysis of both the primary and secondary immune responses (3,8,11,14,15,17). Data have been presented to indicate polyanionic stimulation of both B-cells (10) and T-cells (9).…”