Zoonotic Enterocytozoon bieneusi genotypes found in brazilian sheep

Fiúza, Vagner Ricardo da Silva; Lopes, Carlos Wilson Gomes; Cosendey, Rachel Ingrid Juliboni; Oliveira, Francisco Carlos Rodrigues de; Fayer, Ronald; Santı́n, Mónica

doi:10.1016/j.rvsc.2016.06.006

Cited by 31 publications

(20 citation statements)

References 92 publications

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“…E. bieneusi DNA has already been identified in fecal samples in Brazil collected from humans (FENG et al, 2011), cattle (FIUZA et al, 2016b), birds (CUNHA et al, 2016LALLO et al, 2012), pigs (FIUZA et al, 2015 and sheep (FIUZA et al, 2016a). In addition, microsporidium was also detected in a sample of treated effluent water collected in the region of Campinas, São Paulo, demonstrating the significant possibility of dispersion of this agent even after sewage treatment (YAMASHIRO et al, 2017).…”

Section: Discussionmentioning

confidence: 93%

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Prado

Ramos

Fiúza

et al. 2019

Rev. Bras. Parasitol. Vet.

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Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

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Section: Discussionmentioning

confidence: 93%

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Prado

Ramos

Fiúza

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…Similar rate of infection was reported for E. bieneusi in sheep in Northeast China (13.9%, 68/489) (11), Southwest China (10.6%, 70/661) (12), and Egypt (11.2%, 10/89) (13); while lower infection rates were detected in sheep in East-central China (3.4%, 28/832) (14) and Slovakia (0/33) (15). However, a higher prevalence of E. bieneusi was found in Inner Mongolian (China) lambs (77.8%, 126/162) (16), Swedish lambs (68%, 49/72) (17), Brazilian sheep (19.2%, 24/125) (18), and in sheep from some other studies in China (19)(20)(21)(22). The differences in the prevalence are likely due to differences in the geographic and ecological setup.…”

Section: Discussionmentioning

confidence: 99%

“…The genotype BEB6 identified in cattle in the United States (23), was also identified as the dominant E. bieneusi genotype (13/39, 33.3%) in lambs in the present study. Many other studies demonstrated that the genotype BEB6 was the dominant E. Bieneusi genotype in sheep in Sweden (17), Brazil (18), and China (16,21). Although this genotype was considered to be cattle-specific, it is now recognized as a dominant genotype in humans, animals and birds in a wide geographic distribution, and has zoonotic potential (11,17,19,24,25).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Enterocytozoon bieneusi in Lambs in Oromia Special Zone, Central Ethiopia

Wegayehu

Karim

et al. 2020

Front. Vet. Sci.

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Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and occurs in a wide range of animals. This study was conducted in Central Ethiopia to determine the prevalence and genotypes of E. bieneusi in lambs in order to evaluate their public health significance. Three hundred eighty nine fecal samples were collected and screened using a nested PCR targeting the internal transcribed spacer (ITS) of the ribosomal RNA gene. All positive PCR products were sequenced to determine the genotypes. E. bieneusi was found in 39 (10.03%) of the lambs. Differences in the infection rates among sex and age groups were not significant (P > 0.05). Five ITS genotypes belonging to three known genotypes BEB6, COS-I, and COS-II, and two novel genotypes (ET-L1 and ET-L2) were identified in lambs. All five genotypes identified in the present study clustered within cattle-specific Group 2 in the ITS phylogenetic tree. This first molecular detection and characterization of E. bieneusi in lambs in Ethiopia has identified the need for further studies in humans and other domestic animals in order to determine the public health significance of E. bieneusi in Ethiopia.

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“…There are reports in cattle (Fiuza et al 2016a), pigs (Fiuza et al 2015), sheep (Fiuza et al 2016b), coatis (Lallo et al 2012a), and birds (Lallo et al 2012b, Cunha et al 2016). These findings highlight the risk of human microsporidiosis by zoonotic transmission, since genotypes previously reported in both humans and animals were found in all animals surveyed in these studies, with the exception of coatis.…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, nine studies have used molecular methods to detect this pathogen in HIV-infected patients presenting chronic diarrhoea, and animals including cattle, pigs, chickens, coatis, pigeons, and exotic birds, which highlights the potential risk of transmission to healthy humans (Brasil et al 1998, 2000, Feng et al 2011, Lallo et al 2012a, b, Fiuza et al 2015, 2016a, b, Cunha et al 2016). However, no information regarding E. bieneusi presence in environmental samples in Brazil is available.…”

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confidence: 99%

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Yamashiro

Fiúza

Teixeira

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined by molecular methods.OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a combined system by molecular methods.METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E. bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction.FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent.MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil.

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Zoonotic Enterocytozoon bieneusi genotypes found in brazilian sheep

Cited by 31 publications

References 92 publications

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Molecular Characterization and Phylogenetic Analysis of Enterocytozoon bieneusi in Lambs in Oromia Special Zone, Central Ethiopia

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

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