Zinc-Induced Genotoxic Effects in Root Meristems of Barley Seedlings

Truta, Elena; Gherghel, Daniela; Bara, Ion; Vochița, Gabriela

doi:10.15835/nbha4118943

Cited by 12 publications

(4 citation statements)

References 20 publications

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“…In this study, we detected an increase in PI fluorescence in root tips of ZnONPs exposed wheat seedlings. Previous studies demonstrated that Zn could form stable complexes with nucleic acids, affecting their stability and also cause genotoxicity in plants [30]. As reported by Finger-Teixeira et al [9], the reduction of root growth as observed in this study might also be linked to significant loss of cell viability in wheat seedlings.…”

Section: Discussionsupporting

confidence: 79%

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Prakash¹,

Chung²

2016

Acta Biologica Hungarica

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The effect of zinc oxide nanoparticles (ZnONPs) was studied in wheat (Triticum aestivum L.) seedlings under in vitro exposure conditions. To avoid precipitation of nanoparticles, the seedlings were grown in half strength semisolid Murashige and Skoog medium containing 0, 50, 100, 200, 400 and 500 mg L -1 of ZnONPs. Analysis of zinc (Zn) content showed significant increase in roots. In vivo detection using fluorescent probe Zynpyr-1 indicated accumulation of Zn in primary and lateral root tips. All concentrations of ZnONPs significantly reduced root growth. However, significant decrease in shoot growth was observed only after exposure to 400 and 500 mg L -1 of ZnONPs. The reactive oxygen species and lipid peroxidation levels significantly increased in roots. Significant increase in cell-wall bound peroxidase activity was observed after exposure to 500 mg L -1 of ZnONPs. Histochemical staining with phloroglucinol-HCl showed lignification of root cells upon exposure to 500 mg L -1 of ZnONPs. Treatment with propidium iodide indicated loss of cell viability in root tips of wheat seedlings. These results suggest that redox imbalances, lignification and cell death has resulted in reduction of root growth in wheat seedlings exposed to ZnONPs nanoparticles.

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Section: Discussionsupporting

confidence: 79%

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Prakash¹,

Chung²

2016

Acta Biologica Hungarica

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“…Oladele et al [ 138 ] demonstrated that high levels of Zn (100 mg·L −1 ) in cells resulted in abnormal chromosomes, which was followed by a sticky metaphase and premature separation of chromosomes in bambara groundnut ( Vigna subterranean ). Also, Truta et al [ 139 ] observed that the rate of ana-telophase aberrations was 2-3 times higher than control treatment when barely seedlings ( Hordeum vulgare L.) were treated with 250 to 500 μ M Zn 2+ .…”

Section: Effects Of Some Hms On Plantsmentioning

confidence: 99%

Heavy Metal Stress and Some Mechanisms of Plant Defense Response

Emamverdian

Ding

Mokhberdoran

et al. 2015

The Scientific World Journal

826

310

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Unprecedented bioaccumulation and biomagnification of heavy metals (HMs) in the environment have become a dilemma for all living organisms including plants. HMs at toxic levels have the capability to interact with several vital cellular biomolecules such as nuclear proteins and DNA, leading to excessive augmentation of reactive oxygen species (ROS). This would inflict serious morphological, metabolic, and physiological anomalies in plants ranging from chlorosis of shoot to lipid peroxidation and protein degradation. In response, plants are equipped with a repertoire of mechanisms to counteract heavy metal (HM) toxicity. The key elements of these are chelating metals by forming phytochelatins (PCs) or metallothioneins (MTs) metal complex at the intra- and intercellular level, which is followed by the removal of HM ions from sensitive sites or vacuolar sequestration of ligand-metal complex. Nonenzymatically synthesized compounds such as proline (Pro) are able to strengthen metal-detoxification capacity of intracellular antioxidant enzymes. Another important additive component of plant defense system is symbiotic association with arbuscular mycorrhizal (AM) fungi. AM can effectively immobilize HMs and reduce their uptake by host plants via binding metal ions to hyphal cell wall and excreting several extracellular biomolecules. Additionally, AM fungi can enhance activities of antioxidant defense machinery of plants.

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“…For staining, the root tips were softened in 37% HCl:distilled water solution (1:1) for 25 min, and then maintained in modified carbol-fuchsin dye [53] in a refrigerator. For each variant, we prepared five microscope slides via the squash technique [54], using individual root tips from each germinated caryopsis, which were crushed on the slide, and poured one drop of 45% acetic acid [55]. For each slide, at least 2000 cells and over thirty microscopic fields per slide were analyzed by the same operator using a Euromex IS 1153-EPL microscope (40× objective, Euromex Optics, Arnhem, The Netherlands).…”

Section: Nanotoxicity Testmentioning

confidence: 99%

A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid

Răcuciu,

Oancea,

Barbu-Tudoran

et al. 2024

Materials

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Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by the electrostatic adsorption of HA onto the nanoparticles’ surfaces. A theoretical study carried out with the PM3 method evidenced a dipole moment of 3.34 D and negatively charged atom groups able to participate in interactions with nanoparticle surface cations and surrounding water molecules. The ATR-FTIR spectrum evidenced the hyaluronic acid binding to the surface of the ferrophase, ensuring colloidal stability in the water dispersion. To verify the success of the synthesis and stabilization, HA-MNPs were also characterized using other investigation techniques: TEM, EDS, XRD, DSC, TG, NTA, and VSM. The results showed that the HA-MNPs had a mean physical size of 9.05 nm (TEM investigation), a crystallite dimension of about 8.35 nm (XRD investigation), and a magnetic core diameter of about 8.31 nm (VSM investigation). The HA-MNPs exhibited superparamagnetic behavior, with the magnetization curve showing saturation at a high magnetic field and a very small coercive field, corresponding to the net dominance of single-domain magnetic nanoparticles that were not aggregated with reversible magnetizability. These features satisfy the requirement for magnetic nanoparticles with a small size and good dispersibility for long-term stability. We performed some preliminary tests regarding the nanotoxicity in the environment, and some chromosomal aberrations were found to be induced in corn root meristems, especially in the anaphase and metaphase of mitotic cells. Due to their properties, HA-MNPs also seem to be suitable for use in the biomedical field.

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Zinc-Induced Genotoxic Effects in Root Meristems of Barley Seedlings

Cited by 12 publications

References 20 publications

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Heavy Metal Stress and Some Mechanisms of Plant Defense Response

A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid

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