Determination of zinc oxide nanoparticles toxicity in root growth in wheat (<i>Triticum aestivum</i>L.) seedlings

Prakash, Meppaloor G.; Chung, Ill Min

doi:10.1556/018.67.2016.3.6

Cited by 32 publications

(10 citation statements)

References 32 publications

(37 reference statements)

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“…A correlation between the inhibition of growth and the generation of reactive oxygen species (ROS), resulting in oxidative stress, has often been suggested (Prakash and Chung 2016). This was only partially confirmed in our results, where the highest H 2 O 2 concentration was associated with the highest inhibition of root growth just in some samples.…”

Section: Discussionsupporting

confidence: 80%

An integrated approach to highlight biological responses of Pisum sativum root to nano-TiO2 exposure in a biosolid-amended agricultural soil

Giorgetti

Spanò

Muccifora

et al. 2019

Science of The Total Environment

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This study focused on crop plant response to a simultaneous exposure to biosolid and TiO at micro-and nano-scale, being biosolid one of the major sink of TiO 2 nanoparticles released into the soil environment. We settled an experimental design as much as possible realistic, at microcosm scale, using the crop Pisum sativum. This experimental design supported the hypotheses that the presence of biosolid in the farming soil might influence plant growth and metabolism and that, after TiO 2 spiking, the different dimension and crystal forms of TiO 2 might be otherwise bioavailable and differently interacting with the plant system. To test these hypotheses, we have considered different aspects of the response elicited by TiO 2 and biosolid at cellular and organism level, focusing on the root system, with an integrative approach. In our experimental conditions, the presence of biosolid disturbed plant growth of P. sativum, causing cellular damages at root level, probably through mechanisms not only oxidative stress-dependent but also involving altered signalling processes. These disturbances could depend on non-humified compounds and/or on the presence of toxic elements and of nanoparticles in the biosolid-amended soil. The addition of TiO 2 particles in the sludgeamended soil, further altered plant growth and induced oxidative and ultrastructural damages. Although non typical dose-effect response was detected, the most responsiveness treatments were found for the anatase crystal form, alone or mixed with rutile. Based on ultrastructural observations, we could hypothesise that the toxicity level of TiO 2 nanoparticles may depend on the cell ability to isolate nanoparticles in subcellular compartments, avoiding their interaction with organelles and/or metabolic processes. The results of the present work suggest reflections on the promising practice of soil amendments and on the use of nanomaterials and their safety for food plants and living organisms.

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Section: Discussionsupporting

confidence: 80%

An integrated approach to highlight biological responses of Pisum sativum root to nano-TiO2 exposure in a biosolid-amended agricultural soil

Giorgetti

Spanò

Muccifora

et al. 2019

Science of The Total Environment

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“…These results are similar to the previous report showing the maximum shoot length, root length, FW and DW of sesame seedlings observed in biologically synthesised ZnO-NPs-treated plants [33]. C-ZNPs showed adverse effects on root growth of several plant species like wheat, chickpea, radish, rape and rye grass [34][35][36]. Zinc is an important micronutrient, crucial for growth and development of plant.…”

Section: Effects Of C-znps and G-znps On Seedlings Growth Parameterssupporting

confidence: 90%

Green and chemically synthesized zinc oxide nanoparticles: effects on in-vitro seedlings and callus cultures of Silybum marianum and evaluation of their antimicrobial and anticancer potential

Saeed

Younas

Fazal

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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Zinc oxide nanoparticles (ZnO-NPs) have been produced by physical and chemical methods. Here, the comparative evaluation of both chemically-synthesised ZnO-NPs (C-ZNPs) and in-vitro cultured S. marianum mediated green-synthesised ZnO-NPs (G-ZNPs) were investigated on seed germination frequency, root and shoot growth, callus induction and biochemical profile of medicinally important plant Silybum marianum. Of all the treatments, callus-mediated ZnO-NPs gave optimum results for seed germination (65%), plantlet's root length (4.3 cm), shoot length (5.3 cm) and fresh and dry weights (220.4 g L À1 and 21.23 g L À1 , respectively). Similarly, the accumulation of phenolic (12.3 mg/mg DW) and flavonoid (2.8 mg/mg DW) contents were also enhanced in callus cultures treated with G-ZNPs. We also observed maximum antioxidant activity (99%) in callus cultures treated with G-ZNPs, however, in case of plantlets, these activities were found highest for in-vitro whole plant-mediated ZnO-NPs. Moreover, G-ZNPs also enhanced total protein content (265.32 BSAE/20g FW) in callus cultures. G-ZNPs were further assessed for their effects on several multidrug resistant bacterial strains and human liver carcinoma (HepG2) cells and our findings revealed that callus extracts treated with G-ZNPs show ameliorated antibacterial (highest zone of inhibition (19 mm) against Klebsiella pneumonia) and anticancer (highest cytotoxicity of 64%) activities.

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“…Increasing ROS formations in epidermal cells cause a peroxidation of membrane lipids [ 28 , 34 , 41 , 42 ], membrane damage [ 41 ], and cell apoptosis [ 43 , 44 ]. Excess ROS can also stimulate cell wall lignification and callose deposition [ 44 , 45 ]. As a result, increased cell wall rigidity reduces cell elongation and cell wall permeability and inhibits root elongation [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, our observations did not confirm this, because coleoptile growth was inhibited to the same extent by (Bio)Ag NPs at concentrations of 10 as 40 mg/L ( Figure 3 ). Therefore, other factors such as changes in transpiration/metabolism of phytohormones and increased ROS production [ 45 , 46 , 48 , 49 , 50 ] presumably contribute to this.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Bio-Synthesized Silver Nanoparticles on Germination, Early Seedling Development, and Metabolome of Wheat (Triticum aestivum L.)

et al. 2022

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Changes in the metabolome of germinating seeds and seedlings caused by metal nanoparticles are poorly understood. In the present study, the effects of bio-synthesized silver nanoparticles ((Bio)Ag NPs) on grains germination, early seedlings development, and metabolic profiles of roots, coleoptile, and endosperm of wheat were analyzed. Grains germinated well in (Bio)Ag NPs suspensions at the concentration in the range 10–40 mg/L. However, the growth of coleoptile was inhibited by 25%, regardless of (Bio)Ag NPs concentration tested, whereas the growth of roots gradually slowed down along with the increasing concentration of (Bio)Ag NPs. The deleterious effect of Ag NPs on roots was manifested by their shortening, thickening, browning of roots tips, epidermal cell death, progression from apical meristem up to root hairs zone, and the inhibition of root hair development. (Bio)Ag NPs stimulated ROS production in roots and affected the metabolic profiles of all tissues. Roots accumulated sucrose, maltose, 1-kestose, phosphoric acid, and some amino acids (i.e., proline, aspartate/asparagine, hydroxyproline, and branched-chain amino acids). In coleoptile and endosperm, contrary to roots, the concentration of most metabolites decreased. Moreover, coleoptile accumulated galactose. Changes in the concentration of polar metabolites in seedlings revealed the affection of primary metabolism, disturbances in the mobilization of storage materials, and a translocation of sugars and amino acids from the endosperm to growing seedlings.

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Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Cited by 32 publications

References 32 publications

An integrated approach to highlight biological responses of Pisum sativum root to nano-TiO2 exposure in a biosolid-amended agricultural soil

An integrated approach to highlight biological responses of Pisum sativum root to nano-TiO2 exposure in a biosolid-amended agricultural soil

Green and chemically synthesized zinc oxide nanoparticles: effects on in-vitro seedlings and callus cultures of Silybum marianum and evaluation of their antimicrobial and anticancer potential

The Effect of Bio-Synthesized Silver Nanoparticles on Germination, Early Seedling Development, and Metabolome of Wheat (Triticum aestivum L.)

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