Zigzag microchannel for rigid inertial separation and enrichment (Z-RISE) of cells and particles

Bazaz, Sajad Razavi; Mihandust, Asma; Salomon, Robert N.; Joushani, Hossein Ahmadi Nejad; Li, Wenyan; Amiri, Hoseyn A.; Mirakhorli, Fateme; Zhand, Sareh; Shrestha, Jesus; Miansari, Morteza; Thierry, Benjamin; Jin, Dong-Yan; Warkiani, Majid Ebrahimi

doi:10.1039/d2lc00290f

Cited by 29 publications

(15 citation statements)

References 43 publications

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“…The fundamentals and principles of particle focusing within a zigzag microchannel have been discussed by our group recently. 44 This particular microfluidic device leverages the effects of inertial migration via properly adjusting the inertial lift and Dean drag forces within the zigzag microchannel to separate bacterial cells from larger background cell populations and debris. Inertial migration is defined as the positioning of randomly dispersed particles toward certain equilibrium positions after a sufficiently long particle migration process.…”

Section: Resultsmentioning

confidence: 99%

“…Compared to other serpentine-type microchannels such as curvilinear and square-wave devices, zigzag microfluidics shows superior particle focusing accuracy and efficiency, as we have shown previously. 44 This is because, in zigzag microchannels, cells and particles experience sudden cross-sectional changes (Lagrangian point of view) at the corners after passing each loop of the channel, resulting in alteration of Dean drag and inertial lift force applied to the particles, which is missing in square-wave and curvilinear microchannels, which have the same cross-section throughout the channel length. As the cross-section expands at the corners of zigzag microchannels, the channel aspect ratio increases, resulting in narrower focusing bands and more stable equilibrium positions.…”

Section: Resultsmentioning

confidence: 99%

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Rapid separation of bacteria from primary nasal samples using inertial microfluidics

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Rapid separation of bacteria from primary nasal samples using inertial microfluidics

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“…As an example of the method, we applied it to quantitively assess the performance of 3D printed chip using zigzag geometries that we have previously published [7]. The active mechanism of this device is inertial focusing and achieves the separation based on size.…”

Section: Quantitative Characterisation Using Bead Populations To Iden...mentioning

confidence: 99%

A Method for Rapid, Quantitative Evaluation of Particle Sorting in Microfluidics Using Basic Cytometry Equipment

Salomon

Bazaz

et al. 2023

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This paper describes, in detail, a method that uses flow cytometry to quantitatively characterise the performance of continuous-flow microfluidic devices designed to separate particles. Whilst simple, this approach overcomes many of the issues with the current commonly utilised methods (high-speed fluorescent imaging, or cell counting via either a hemocytometer or a cell counter), as it can accurately assess device performance even in complex, high concentration mixtures in a way that was previously not possible. Uniquely, this approach takes advantage of pulse processing in flow cytometry to allow quantitation of cell separation efficiencies and resulting sample purities on both single cells as well as cell clusters (such as circulating tumour cell (CTC) clusters). Furthermore, it can readily be combined with cell surface phenotyping to measure separation efficiencies and purities in complex cell mixtures. This method will facilitate the rapid development of a raft of continuous flow microfluidic devices, will be helpful in testing novel separation devices for biologically relevant clusters of cells such as CTC clusters, and will provide a quantitative assessment of device performance in complex samples, which was previously impossible.

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“…This system had a very high throughput (1.2 mL/min volumetric flow rate) and took less than 9 min for Cycle 1 (10 mL) at the sampling inlet (corresponding to 5 mL of whole blood) and 3 min for Cycle 2. Bazaz et al [107] investigated zigzag microchannels for rigid inertial separation and enrichment of cells and particles. These devices provide improved particle separation via an induced secondary recirculating (Dean) flow perpendicular to the main flow direction.…”

Section: Separation By a Combination Of Hydrodynamic Forcesmentioning

confidence: 99%

Microfluidic Systems for Blood and Blood Cell Characterization

2022

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A laboratory blood test is vital for assessing a patient’s health and disease status. Advances in microfluidic technology have opened the door for on-chip blood analysis. Currently, microfluidic devices can reproduce myriad routine laboratory blood tests. Considerable progress has been made in microfluidic cytometry, blood cell separation, and characterization. Along with the usual clinical parameters, microfluidics makes it possible to determine the physical properties of blood and blood cells. We review recent advances in microfluidic systems for measuring the physical properties and biophysical characteristics of blood and blood cells. Added emphasis is placed on multifunctional platforms that combine several microfluidic technologies for effective cell characterization. The combination of hydrodynamic, optical, electromagnetic, and/or acoustic methods in a microfluidic device facilitates the precise determination of various physical properties of blood and blood cells. We analyzed the physical quantities that are measured by microfluidic devices and the parameters that are determined through these measurements. We discuss unexplored problems and present our perspectives on the long-term challenges and trends associated with the application of microfluidics in clinical laboratories. We expect the characterization of the physical properties of blood and blood cells in a microfluidic environment to be considered a standard blood test in the future.

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Zigzag microchannel for rigid inertial separation and enrichment (Z-RISE) of cells and particles

Abstract: Separation and enrichment of target cells prior to downstream analyses is an essential pre-treatment step in many biomedical and clinical assays. Separation techniques utilizing simple, cost-effective, and user-friendly devices are...

Cited by 29 publications

References 43 publications

Rapid separation of bacteria from primary nasal samples using inertial microfluidics

Rapid separation of bacteria from primary nasal samples using inertial microfluidics

A Method for Rapid, Quantitative Evaluation of Particle Sorting in Microfluidics Using Basic Cytometry Equipment

Microfluidic Systems for Blood and Blood Cell Characterization

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