Rapid separation of bacteria from primary nasal samples using inertial microfluidics

Abstract

Microbial populations play a crucial role in human health and the development of many diseases. These diseases often arise from the explosive proliferation of opportunistic bacteria, such as those in...

Minimum Minutes Machine-Learning Microfluidic Microbe Monitoring Method (M7)

“…The channel aspect ratio (W/H) affects the Dean flow intensity. 26 The smaller the aspect ratio, the weaker the swirl intensity. Therefore, adjusting the aspect ratio can control the particle balance position.…”

“…The Dean flow intensity caused by the curved channel is evaluated using the Dean number ( D e ), which is calculated as italicD normale = Re normalc D h 2 R where Re c is the Reynolds number, R is the radius of curvature of the curved channel, D h is the hydraulic diameter defined as 2 WH /( W + H ), W is the channel width, and H is the channel height. The channel aspect ratio ( W / H ) affects the Dean flow intensity . The smaller the aspect ratio, the weaker the swirl intensity.…”

A Novel Strategy for Square-Wave Micromixers: A Survey of RBC Lysis for Further Biological Analysis

“…Microfluidics, also known as lab-on-a-chip, is the science studying the flow behavior at the microscale. , Flow manipulation within microfluidic devices such as micromixers, − microdroplet generators, − and microseparators − is pursued to achieve the desired output. These systems have found their functionality in various biorelated analyses and engineering. − In many of these applications, efficient and well-controlled mixing is crucial in successfully satisfying the aim of the desired study. , Therefore, micromixers are one of the most pivotal parts of lab-on-a-chip systems.…”

Advancing in situ single-cell microbiological analysis through a microwell droplet array with a gradual open sidewall

“…[5][6][7] In the field of microbial physiology, cell phenotyping and histological analysis, the SCA technique has played a crucial role in improved pathogen detection tools and processes depending on the advantage in pathogen isolation and detection systems in recent years. [8][9][10] The preferred method for bacterial single-cell analysis in SCA technology is droplet microfluidics, which is effective manipulation on a chip and enables multiple analyses of individual droplets for ultra-high throughput acquisition of single cells. [11][12][13][14] Among them, microwell droplet arrays have inherent advantages over continuous flow droplets in that they allow for in situ detection by fixed physical microwells, which create a more stable microenvironment, prevent sample contamination, and allow for in situ culture and colony analysis of single bacterial cells through cell attachment and diffusion.…”

“…5–7 In the field of microbial physiology, cell phenotyping and histological analysis, the SCA technique has played a crucial role in improved pathogen detection tools and processes depending on the advantage in pathogen isolation and detection systems in recent years. 8–10…”