Zebrafish models for functional and toxicological screening of nanoscale drug delivery systems: promoting preclinical applications

Lee, Keon Yong; Jang, Gun Hyuk; Byun, Cho Hyun; Jeun, Minhong; Searson, Peter C.; Lee, Kwan H.

doi:10.1042/bsr20170199

Cited by 51 publications

(44 citation statements)

References 112 publications

(125 reference statements)

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“…It has been reported that zebrafish is an excellent model for that purpose since it mimics the complexity of interactions in the human body due to its high degree of homology to human genome and biological systems. Its embryos can be used to monitor phenotypic and genotypic abnormalities upon exposure to toxic agents (Lee et al, 2017). Toxicresponse similarities between zebrafish and mammals have been renowned for small molecules that cause endocrine disruption, reproductive toxicity, behavioral defects, teratogenesis, carcinogenesis, cardiotoxicity, ototoxicity, liver toxicity and others (Zon and Peterson, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development

Adenan¹,

Yazan²,

Christianus³

et al. 2018

J. Cell Anim. Biol.

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Kelulut honey (KH) is a type of honey with various pharmacological properties that can be found in Malaysia. Nevertheless, the safety aspects of this honey have not been adequately addressed. This study evaluated the developmental toxicity of KH from Trigona sp on zebrafish (Danio rerio) embryos. Viable zebrafish embryos at 3 hours post fertilization (hpf) (early stage) and 24 hpf (organ development stage) were treated with KH (1 to 20 mg/mL). The embryos were examined for morphological abnormalities and viability until 96 h of KH treatment. Coagulated embryos were identified after treatment with KH (≥10 mg/mL) for 3 hpf group and KH (≥12 mg/mL) for 24 hpf group. The LC 50 values of KH at 96 h of exposure for the 3 hpf and 24 hpf group were 12.52 and 16.36 mg/mL, respectively. The maximum allowable concentration (MAC) for KH on 3 hpf and 24 hpf group were 0.63 and 0.82 mg/mL, respectively. The irregular cardiac rate of the embryos was noted at 10 mg/mL for 3 hpf group and 13 mg/mL of KH for 24 hpf group. In summary, the early stage embryo (3 hpf group) was more sensitive to KH than the one of later stage (24 hpf group). It indicates that serious precautions should be taken into account in the use of any material including natural product, be it food or supplement, especially in the early stage of life.

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Section: Discussionmentioning

confidence: 99%

Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development

Adenan¹,

Yazan²,

Christianus³

et al. 2018

J. Cell Anim. Biol.

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“…Biosynthesized nanoparticles have been described for their ability to inhibit/destroy microbial cells. Nanoparticles may be good source for delivery of herbal medicines in the field of health promotion [35].…”

Section: Evaluation Of Antimicrobial Activitiesmentioning

confidence: 99%

Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

Singh

Kumar

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Zebrafish (Danio rerio) develop most of the main organ systems present in mammals during the first week of life; some organs and tissues have been shown to be similar to those of mammals at anatomic, physiological, and molecular levels [17]. As zebrafish larvae develop in a short period of time, ex utero, and are transparent, they are ideal for nano-particle and drug analysis with noninvasive techniques [18].…”

Section: Introductionmentioning

confidence: 99%

Nano-formulation for topical treatment of precancerous lesions: skin penetration, in vitro, and in vivo toxicological evaluation

Calienni

Temprana

Prieto

et al. 2017

Drug Deliv. and Transl. Res.

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With the aim of improving the topical delivery of the antineoplastic drug 5-fluorouracil (5FU), it was loaded into ultradeformable liposomes composed of soy phosphatidylcholine and sodium cholate (UDL-5FU). The liposome populations had a mean size of 70 nm without significant changes in 56 days, and the ultradeformable formulations were up to 324-fold more elastic than conventional liposomes. The interaction between 5FU and the liposomal membrane was studied by three methods, and also release profile was obtained. UDL-5FU did penetrate the stratum corneum of human skin. At in vitro experiments, the formulation was more toxic on a human melanoma-derived than on a human keratinocyte-derived cell line. Cells captured liposomes by metabolically active processes. In vivo toxicity experiments were carried out in zebrafish (Danio rerio) larvae by studying the swimming activity, morphological changes, and alterations in the heart rate after incubation. UDL-5FU was more toxic than free 5FU. Therefore, this nano-formulation could be useful for topical application in deep skin precancerous lesions with advantages over current treatments. This is the first work that assessed the induction of apoptosis, skin penetration in a Saarbrücken penetration model, and the toxicological effects in vivo of an ultradeformable 5FU-loaded formulation.

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Zebrafish models for functional and toxicological screening of nanoscale drug delivery systems: promoting preclinical applications

Cited by 51 publications

References 112 publications

Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development

Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development

Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

Nano-formulation for topical treatment of precancerous lesions: skin penetration, in vitro, and in vivo toxicological evaluation

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