Green synthesis of silver nanoparticles using aqueous leaf extract of <i>Premna integrifolia</i> (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

Singh, Chandrashekhar; Kumar, Jitendra; Kumar, Pradeep; Chauhan, Brijesh Singh; Tiwari, Kavindra Nath; Mishra, Sunil Kumar; Srikrishna, Saripella; Saini, Rajesh; Nath, Gopal; Singh, Jasmeet

doi:10.1080/13102818.2019.1577699

Cited by 54 publications

(28 citation statements)

References 37 publications

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“…Again, this supports the view that tiny size of nanoparticles may assist in easy penetration of microbial cell wall and destroy various organelles. Biosynthesized small NPs have been identified as being able to inhibit/destroy microbial cells (Singh et al 2019). In agreement with previous experimental results (Saranya and Gowrie 2016), this study suggested that the application of AgNPs synthesized from leaves and fruits aqueous extract of C. equisetifolia, could be used as an effective antimicrobial agent.…”

Section: Discussionsupporting

confidence: 91%

Green Synthesis of Ag Nanoparticles from Aqueous Extracts of Leaves and Fruit of Casuarina equisetifolia against Candida albicans and other Clinical Isolates

Moustafa¹

2021

IJAB

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The prevalence of the infectious diseases caused by Candida species and other human pathogenic microbes has led to the discovery of some natural agents against multidrug resistant microbes. Therefore, this research was designated to determine the capability of synthesized nanoparticles (NPs) from aqueous extract of leaves and fruits of Casuarina equisetifolia as anticandidal and antibacterial against some microbes. Silver NPs (AgNPs) were successfully gained from aqueous leaves and fruit extracts of C. equisetifolia which is defined on the basis of UV–visible spectroscopy, X-ray diffraction analysis and scanning electron microscope. The results showed that leaves and fruits extracts of C. equisetifolia acts as an excellent capping agent. XRD based on the FWHM analysis showed that AgCl and Ag had an average NPs size of 90.97 nm and 71.28 nm, respectively for fruits and 15.33 nm and 14.01 nm, respectively for leaves. UV–visible spectroscopy showed a maximum absorbance at 442 nm for fruits and 433 nm for leaves. SEM showed that the size of NPs from leaves lied in between of (30 to180 nm) and for fruits (70 to 250 nm). Candida albicans was severely affected by NPs of leaves with inhibition zone (3.03± 1.61 cm) and NPs of fruits had (1.37± 0.15 cm) inhibition zone. Nanoparticles from leaves exhibited maximum activity against P. mirabilis (3.52± 0.13 cm) and low activities against M. luteus (1.50±0.18 cm) inhibition zones. In conclusion, these eco-friendly synthesized AgNPs from leaves and fruits of C. equisetifolia could be used as competitive alternative natural drugs than conventional synthetic chemicals. © 2021 Friends Science Publishers

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Section: Discussionsupporting

confidence: 91%

Green Synthesis of Ag Nanoparticles from Aqueous Extracts of Leaves and Fruit of Casuarina equisetifolia against Candida albicans and other Clinical Isolates

Moustafa¹

2021

IJAB

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“…In the low wavenumber region, they showed a shoulder at 1717 cm −1 which can be ascribed to the -COOH stretching of free carboxylic molecules, along with two strong bands, at 1640 cm −1 and 1387 cm −1 in the NPs from Leccino , and 1600 cm −1 and 1356 cm −1 in the NPs from Carolea . Such a double band can be accounted for by the signals of the antisymmetric and symmetric -COO- stretching of carboxylic molecules, respectively, coordinated to the surface Ag atoms [56,57] and thus responsible for the stabilization of the NPs in aqueous solution. Indeed, the same bands were observed also in the FTIR-ATR spectra of AgNPs, synthesized by the colloidal chemical route, by reduction of silver precursor in the presence of sodium citrate and tannic acid surfactants, which were coordinated to the AgNPs surface by their carboxyl groups.…”

Section: Resultsmentioning

confidence: 99%

Cultivar-Dependent Anticancer and Antibacterial Properties of Silver Nanoparticles Synthesized Using Leaves of Different Olea Europaea Trees

et al. 2019

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The green synthesis of nanoparticles (NPs) is currently under worldwide investigation as an eco-friendly alternative to traditional routes (NPs): the absence of toxic solvents and catalysts make it suitable in the design of promising nanomaterials for nanomedicine applications. In this work, we used the extracts collected from leaves of two cultivars (Leccino and Carolea) belonging to the species Olea Europaea, to synthesize silver NPs (AgNPs) in different pH conditions and low temperature. NPs underwent full morphological characterization with the aim to define a suitable protocol to obtain a monodispersed population of AgNPs. Afterwards, to validate the reproducibility of the mentioned synthetic procedure, we moved on to another Mediterranean plant, the Laurus Nobilis. Interestingly, the NPs obtained using the two olive cultivars produced NPs with different shape and size, strictly depending on the cultivar selected and pH. Furthermore, the potential ability to inhibit the growth of two woman cancer cells (breast adenocarcinoma cells, MCF-7 and human cervical epithelioid carcinoma, HeLa) were assessed for these AgNPs, as well as their capability to mitigate the bacteria concentration in samples of contaminated well water. Our results showed that toxicity was stronger when MCF-7 and Hela cells were exposed to AgNPs derived from Carolea obtained at pH 7 presenting irregular shape; on the other hand, greater antibacterial effect was revealed using AgNPs obtained at pH 8 (smaller and monodispersed) on well water, enriched with bacteria and coliforms.

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“…Given the predominance of polyphenols like flavonoids and xanthones in fresh coconut inflorescence sap, it could be assumed that these compounds could be mainly involved in synthesis of NPs. Many studies have reported the role of polyphenols in the promotion of green synthesis of metal NPs (Tao et al, 2016;Jigaysa and Rajput, 2018;Singh et al, 2019;Latif et al, 2019).…”

Section: Metabolomic Profiling Of Coconut Inflorescence Sapmentioning

confidence: 99%

Coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial properties

Rajesh

Muralikrishna

Nair

et al. 2019

Preprint

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Green synthesis of nanoparticles (NPs) involves the use of diverse extracts of biological origin as substrates to synthesize nanoparticles and can overcome the hazards associated with chemical 2 methods. Coconut inflorescence sap, which is unfermented phloem sap obtained by tapping of coconut inflorescence, is a rich source of sugars and secondary metabolites. In this study, coconut inflorescence sap was used to synthesize silver nanoparticles (AgNPs). We have initially undertaken metabolomic profiling of coconut inflorescence sap from West Coast Tall cultivar to delineate its individual components. Secondary metabolites constituted the major portion of the inflorescence sap along with sugars, lipids and, peptides. The concentration of silver nitrate, inflorescence sap and incubation temperature for synthesis of AgNPs were optimized. Incubating the reaction mixture at 40ºC was found to enhance AgNP synthesis. The AgNPs synthesized were characterized using UV-Visible spectrophotometry, X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM) and Transmission Electron Microscopy (TEM). Antimicrobial property of AgNP was tested in tissue culture of arecanut (Areca catechu L.) where bacterial contamination (Bacillus pumilus) was a frequent occurrence. Significant reduction in the contamination was observed when plantlets were treated with aqueous solutions of 0.01, 0.02 and 0.03% of AgNPs for one hour. Notably, treatment with AgNPs did not affect growth and development of the arecanut plantlets. Cytotoxicity of AgNPs was quantified in HeLa cells. Viability (%) of HeLa cells declined significantly at 10 ppm concentration of AgNP and complete mortality was observed at 60 ppm. Antimicrobial properties of AgNPs synthesized from inflorescence sap were also evaluated and confirmed in human pathogenic bacteria viz., Salmonella sp., Vibrio parahaemolyticus, and Escherichia coli. The study concludes that unfermented inflorescence sap, with above neutral pH, serves as an excellent reducing agent to synthesize AgNPs from Ag+.3

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Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

Abstract: View related articles View Crossmark data Citing articles: 7 View citing articles Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

Cited by 54 publications

References 37 publications

Green Synthesis of Ag Nanoparticles from Aqueous Extracts of Leaves and Fruit of Casuarina equisetifolia against Candida albicans and other Clinical Isolates

Green Synthesis of Ag Nanoparticles from Aqueous Extracts of Leaves and Fruit of Casuarina equisetifolia against Candida albicans and other Clinical Isolates

Cultivar-Dependent Anticancer and Antibacterial Properties of Silver Nanoparticles Synthesized Using Leaves of Different Olea Europaea Trees

Coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial properties

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