Youngiasides A and C Isolated from Youngia denticulatum Inhibit UVB-Induced MMP Expression and Promote Type I Procollagen Production via Repression of MAPK/AP-1/NF-κB and Activation of AMPK/Nrf2 in HaCaT Cells and Human Dermal Fibroblasts

Abstract

This study investigated the effects of youngiaside A (YA), youngiaside C (YC), and Youngia denticulatum extract (YDE) on extrinsic aging and assessed its molecular mechanisms in UVB-irradiated HaCaT keratinocytes and human dermal fibroblasts (HDFs). The results showed that YA, YC, and YDE decreased matrix metalloproteinase (MMP) expression and production in HaCaT cell and HDFs and increased collagen expression and production in HDFs. In addition, YA, YC, and YDE significantly increased antioxidant enzyme expression, thereby down-regulating UVB-induced reactive oxygen species (ROS) production and ROS-induced mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) signaling in HaCaT cells. Furthermore, YA, YC, and YDE reduced phosphorylation of IκBα and IKKα/β, blocked nuclear factor-κB (NF-κB) p65 nuclear translocation, and strongly suppressed pro-inflammatory mediators. Finally, YA, YC, and YDE augmented UVB-induced adenosine monophosphate activated protein kinase (AMPK) phosphorylation and YA and YC did not inhibit MMP-1 production in AMPK inhibitor or nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA-treated HaCaT cells. The results suggest that these compounds could be potential therapeutic agents for prevention and treatment of skin photoaging.

Upregulation of Matrix Metalloproteinase-9 in Primary Cultured Rat Astrocytes Induced by 2-Chloroethanol Via MAPK Signal Pathways

“…In this study, inhibition of p-p38, p-ERK1/2 and p-JNK1/2 all markedly attenuated MMP-9 expression in 2-CE exposed astrocytes, suggesting that all three kinds of MAPK signal pathways were activated, and collectively involved in modulation of MMP-9 expression in 2-CE exposed astrocytes. Until now, there is a large body of evidence to suggest that MMP-9 expression is regulated by p38, ERKs and JNKs in different cell types (Lee et al, 2014; Kim et al, 2015; Lian et al, 2015). However, as far as we know, this is the first time to discuss the molecular mechanisms of MMP-9 expression regulation in astrocytes exposed to 2-CE.…”

Protective activity of caffeic acid and sinapic acid against UVB-induced photoaging in human fibroblasts

“…Oxidative stress is related to elevated intracellular ROS levels, which causes damage to macromolecules and interferes with the normal signaling cascade (Schieber & Chandel, ). UVB‐induced high levels of ROS have been reported to induce skin fibroblasts to secrete high levels of MMP‐1, resulting in an increase in collagen degradation (Kim et al, ). In the present study, exposure to UVB irradiation (30 mJ/cm 2 ) significantly increased fluorescence intensity, and this increase was significantly reduced by CA and SA treatment (Figure a).…”

“…Fibroblasts are the major cell component of the dermis, which play a key role in the metabolism of extracellular matrix proteins such as type I collagen (Yamaba et al, ). Overexposure to solar UVB leads to intracellular signaling through stimulation of mitogen‐activated protein kinases (MAPKs) induced by excessive generation of reactive oxygen species (ROS) in early‐stage radiation exposure (Kim, Park, Lee, Lim, & Nho, ). Nuclear factor κB (NFκB) is a downstream effector of MAPKs; it reduces collagen synthesis, and increases the levels and activity of matrix metalloproteinases (MMPs) (Philips et al, ).…”

Paeonol extracted from Paeonia suffruticosa Andr. ameliorated UVB‐induced skin photoaging via DLD/Nrf2/ARE and MAPK/AP‐1 pathway

“…GA = gallic acid [Colour figure can be viewed at wileyonlinelibrary.com] be associated with lots of pathologies, including aging caused by the alteration of energy metabolism (Bratic & Trifunovic, 2010). Nrf2 and ARE have been reported to be the key regulators response to oxidative stress (M. Kim, Park, Lee, Lim, & Nho, 2015), and a variety of natural products have been shown to promote the translocation of Nrf2 to the nucleus. For example, Paeonia lactiflora Pall., a peony species, was reported to protect against alpha-naphthylisothiocyanate (ANIT)induced cholestasis by activating the Nrf2 pathway (Ma et al, 2015).…”