Protective activity of caffeic acid and sinapic acid against UVB-induced photoaging in human fibroblasts

Abstract

In this study, the protective effects of caffeic acid (CA) and sinapic acid (SA) on photoaging in human skin fibroblasts induced by UVB irradiation (30 mJ/cm2) were examined. The results revealed that exposure to UVB irradiation reduced cell viability by approximately 33% compared with the non‐UVB irradiated control. However, CA and SA blocked the UVB irradiation‐induced cytotoxicity greater than the other phenolic acids. CA and SA also significantly inhibited the release of matrix metalloproteinase 1 (MMP‐1) and reduced the expression of MMP‐1 mRNA in UVB‐irradiated Hs68 cells. Furthermore, CA and SA reduced UVB‐induced production of reactive oxygen species (ROS) and collagen degradation in Hs68 cells. Finally, CA and SA effectively downregulated activation of the UVB‐induced mitogen‐activated protein kinases (MAPKs) and nuclear factor κB (NFκB) signaling pathways. These results imply that hydroxylated cinnamate derivatives can be therapeutic agents against UVB‐induced skin photoaging. Practical applications Hydroxylated cinnamate derivatives such as CA and SA have a high cytoprotective effect against UV irradiation‐induced photoaging in human fibroblasts via the inactivation of the MAPKs/NFκB signaling pathway. The present study suggests that CA and SA may be useful in therapeutic and cosmetic applications for the treatment of skin photoaging.

Protective Effects of Unsaponifiable Matter from Perilla Seed Meal on UVB-induced Damages and the Underlying Mechanisms in Human Skin Fibroblasts

“…The expression levels of proteins were confirmed as described elsewhere [19]. Proteins were separated and transferred to a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK).…”

The Potential of Caffeic Acid Lipid Nanoparticulate Systems for Skin Application: In Vitro Assays to Assess Delivery and Antioxidant Effect

“…Among them, caffeic acid (CA), naturally found in coffee, fruits, plants, oils, grapes, and tea, is a hydroxycinnamic acid characterized by strong antioxidant power, due to radical scavenging activity and the inhibition of lipid peroxidation [4][5][6]. Thanks to this potential, CA can be employed in the treatment of UVB-induced photoaging and in the chemoprevention of malignant melanoma [7][8][9]. However, the poor water solubility and chemical stability of CA makes necessary the use of specialized delivery systems suitable for its solubilization, protection from degradation, and the maintenance of antioxidative power [10][11][12][13].…”

Callus induction and phytochemical profiling of Yucca carnerosana (Trel.) McKelvey obtained from in vitro cultures

“…On the other hand, compound 44 was detected in all samples, and it was assigned as sinapic acid as proposed by Cabañas-García et al (2019) and Liu et al (2015). For this metabolite, its therapeutic effect against UVB-induced photo-aging of the skin has been proposed (Jeon et al, 2019). Compound 45 was assigned as magnoloside U as reported previously (Cabañas-García et al, 2021), and compound 46 was assigned as 3,4,5-triacetyloxy-6-[(4-oxo-2,3-dihydro-cyclopentachromen-7-yl)oxy]oxan-2yl]methyl acetate since pseudomolecular ion at m/z: 531.1505 yielded one fragment at m/z: 134.0365, and compound 47 was proposed as the glycosylated flavonoid naringenin 7-O-rutinoside due to the presence of one main fragment at m/z: 266.0659, generated by the loss of the glycosidic moiety.…”