Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua

Baldi, Ashish; Dixit, Vinod Kumar

doi:10.1016/j.biortech.2007.06.061

Cited by 155 publications

(90 citation statements)

References 37 publications

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“…The time frame of 48 h may also have been too short to see the full effect of treatment on final artemisinin concentration. The limited effects of COS and SA on concentrations of artemisinin in full grown plants in our study was different from former reports made on non-adult plants [18], and this suggests that signaling molecules may act differently in A. annua depending on the plant model. The use of elicitors therefore may not be a universal method for increasing artemisinin yield from A. annua plants.…”

Section: Resultscontrasting

confidence: 99%

“…In an experiment conducted on hairy root cultures of A. annua, artemisinin production was promoted markedly (6-fold higher than the control) by chitosan (polymer of COS) addition [17]. Whereas, other research reported that chitosan had no effect on artemisinin accumulation in A. annua suspension cultures [18]. So, these reports together with our results suggest that SA and COS treatments functions quite differently in different plant cultures.…”

Section: Resultssupporting

confidence: 48%

See 1 more Smart Citation

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Yin¹,

Kjær²,

Fretté³

et al. 2012

Process Biochemistry

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 48%

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Yin¹,

Kjær²,

Fretté³

et al. 2012

Process Biochemistry

View full text Add to dashboard Cite

“…Compared with controls, 1.0 mmol/L SA could increase the content of artemisinic acid, artemisinin, and dihydroartemisinic acid (Pu et al, 2009;Aftab et al, 2010;Guo et al, 2010). In addition, the artemisinin content of cell suspensions of A. annua treated with 20-50 mg/L (140-360 mmol/L) SA increased significantly, and the highest content was nearly 6-fold higher than that of controls (Baldi and Dixit, 2008). Genes in the SA biosynthetic pathway are considered to be involved in SA production, plant development, and secondary metabolism (Loake and Grant, 2007).…”

Section: Introductionmentioning

confidence: 83%

Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua

Wang

Zhang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Isochorismate synthase (ICS) is a crucial enzyme in the salicylic acid (SA) synthesis pathway. The full-length complementary DNA (cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene, named AaICS1, contained a 1710-bp open reading frame, which encoded a protein with 570 amino acids. Bioinformatics and comparative study revealed that the polypeptide protein of AaICS1 had high homology with ICSs from other plant species. Southern blot analysis suggested that AaICS1 might be a single-copy gene. Analysis of the 1470-bp promoter of AaICS1 identified distinct cis-acting regulatory elements, including TC-rich repeats, MYB binding site (MBS), and TCA-elements. An analysis of AaICS1 transcript levels in multifarious tissues of A. annua using quantitative real-time polymerase chain reaction (qRT-PCR) showed that old leaves had the highest transcription levels. AaICS1 was up-regulated under wounding, drought, salinity, and SA treatments. This was corroborated by the presence of the predicted cis-acting elements in the promoter region of AaICS1. Overexpressing transgenic plants and RNA interference transgenic lines of AaICS1 were generated and their expression was compared. High-performance liquid chromatography (HPLC) results from leaf tissue of transgenic A. annua showed an increase in artemisinin content in the overexpressing plants. These results confirm that AaICS1 is involved in the isochorismate pathway.

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“…In cultural A. annua cells, supplementation with 10 mg/L GA3 increases artemisinin content as 6-fold as the control (Baldi et al 2008). GA3-treated A. annua plants have also significant increases of artemisinin content (Fulzele et al 1995;Paniego and Giulietti 1996).…”

Section: Ga3 and Other Phytohormonesmentioning

confidence: 91%

An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

Jiang

Gao

Liao

et al. 2015

Preprint

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Artemisinin is accumulated in wormwood (Artemisia annua) with uncertain ecological implications. Here, we suggest that artemisinin is generated in response to biotic/abiotic stress, during which dihydroartemisinic acid, a direct artemisinin precursor, quenches singlet oxygen ( 1 O2), one kind of reactive oxygen species. Evidence supporting artemisinin as a sink of 1 O2 emerges from that volatile isoprenoids protect plants from biotic/abiotic stress; biotic/abiotic stress induces artemisinin biosynthesis; and stress signaling pathways are involved in the biosynthesis of volatile isoprenoids among plants as well as the biosynthesis of artemisinin in A. annua. In this review, we address the ecological implication of glandular trichome-sequestered artemisinin as a sink sink of biotic/abiotic stress-triggered 1 O2, and also summarize the cumulating data on the transcriptomic and metabolic profiling of stress-enhanced artemisinin production upon eliciting 1 O2 omission from chloroplasts and initiating retrograde 1 O2 signaling from chloroplasts to nuclei.

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Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua

Cited by 155 publications

References 37 publications

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua

An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

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