Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Yin, Heng; Kjær, Anders; Fretté, Xavier; Du, Yifeng; Christensen, Lars Porskjær; Jensen, Martin; Grevsen, Kai

doi:10.1016/j.procbio.2011.12.020

Cited by 18 publications

(7 citation statements)

References 19 publications

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“…For the chitosan as a stimulant, chitosan is known to elicit activities leading to a variety of defensive responses in host plants to microbial infections, including the accumulation of phytoalexins, pathogen related proteins, callus formation and accumulation of secondary metabolite (Yin et al, 2012). This study found that the most effectivechitosan concentrations for inducing vinblastine and vincristine in C. roseus cell culture were 100 to 250 mg/L because itinduced programmed cell death and hypersensitive-associated responses in plants cell when concentration increased (Vasil'ev et al, 2009) or chitosan toxicity to the living cells or might be due to the phytotoxic action of vinblastine and vincristine on the cells (Amborabé et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Chitosan Elicitation for Enhancing of Vincristine and Vinblastine Accumulation in Cell Culture of Catharanthus roseus (L.) G. Don

Pliankong¹,

Suksa-Ard²,

Wannakrairoj³

2018

JAS

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Catharanthus roseus (L.) G. Don is an important herbal plant. There are two important alkaloids, vinblastine and vincristine, use in anti-cancer drugs. In this study production of the two alkaloids was enhanced in C. roseus cell cultures, in a Murashige and Skoog (MS) liquid medium supplemented with 1.5 mg/L 2,4-D, 0.5 mg/L kinetin and 30 g/L sucrose, by adding 0, 50, 100, 250 or 500 mg/L medium molecular weight chitosan or chitosan derived from shrimp shell. After 14 days of culture, the cell suspension at stationary phase in the 100 mg/L medium molecular weight chitosan could produce the highest amounts of vinblastine and vincristine at 4.15 and 5.48 µg/mg cell dry weight, respectively. At the same time, the controls (0 mg/L chitosan) produced the two alkaloids at only 2.43 and 2.49 µg/mg cell dry weight, respectively. For chitosan from shrimp shell, it was found that 100 mg/L chitosan could lead to the highest quantity of 4.09 µg vinblastine/mg cell dry weight. The highest amount of 5.47 µg vincristine/mg cell dry weight was obtained when 250 mg/L chitosan was added.

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Section: Discussionmentioning

confidence: 99%

Chitosan Elicitation for Enhancing of Vincristine and Vinblastine Accumulation in Cell Culture of Catharanthus roseus (L.) G. Don

Pliankong¹,

Suksa-Ard²,

Wannakrairoj³

2018

JAS

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“…Recently, SA is widely used as an elicitor of secondary metabolite biosynthesis in plant cultures. For example, exogenous applications of SA induced the biosynthesis of coumarins in Matricaria chamomilla L. [ 79 ], taxane in Taxus chinensis Rehder [ 80 ], saponins in ginseng [ 81 ], phenolic acids including rosmarinic acid in Salvia miltiorrhiza [ 66 , 82 ], and artemisin in Artemisia annua L. cultures [ 83 ]. A number of recent mechanistic studies have been carried out to elucidate connective pathways between SA challenge and the induction of metabolic pathways of secondary metabolites in plant cultures [ 82 ].…”

Section: Redox Regulation Of Secondary Metabolite Synthesis In Plamentioning

confidence: 99%

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Korkina¹,

Mayer²,

Luca³

2017

Biomolecules

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Recently, aggressive advertisement claimed a “magic role” for plant stem cells in human skin rejuvenation. This review aims to shed light on the scientific background suggesting feasibility of using plant cells as a basis of anti-age cosmetics. When meristem cell cultures obtained from medicinal plants are exposed to appropriate elicitors/stressors (ultraviolet, ultrasound ultraviolet (UV), ultrasonic waves, microbial/insect metabolites, heavy metals, organic toxins, nutrient deprivation, etc.), a protective/adaptive response initiates the biosynthesis of secondary metabolites. Highly bioavailable and biocompatible to human cells, low-molecular weight plant secondary metabolites share structural/functional similarities with human non-protein regulatory hormones, neurotransmitters, pigments, polyamines, amino-/fatty acids. Their redox-regulated biosynthesis triggers in turn plant cell antioxidant and detoxification molecular mechanisms resembling human cell pathways. Easily isolated in relatively large quantities from contaminant-free cell cultures, plant metabolites target skin ageing mechanisms, above all redox imbalance. Perfect modulators of cutaneous oxidative state via direct/indirect antioxidant action, free radical scavenging, UV protection, and transition-metal chelation, they are ideal candidates to restore photochemical/redox/immune/metabolic barriers, gradually deteriorating in the ageing skin. The industrial production of plant meristem cell metabolites is toxicologically and ecologically sustainable for fully “biological” anti-age cosmetics.

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“…Exogenous application of SA in wheat seedlings [40], leaves of Brassica napus under nickel stress [41], and navel oranges [42] all enhanced their antioxidant systems. Exogenous application of SA also increased benzophenanthridine alkaloid levels in Eschscholtzia californica suspension cultures [43], tropane alkaloid levels in adventitious root cultures of Serapias parviflora [44], artemisinin levels in Artemisia annua [45], flavonolignan levels in the hairy root cultures of Silybum marianum [46], and the levels of phenolic compounds in S. miltiorrhiza cell cultures [47]. In P. vulgaris hairy roots, 6.9 mg L −1 SA was suitable for the elicitation of RA, and treatment with SA led to an increase in RA (1.5-fold) content and increased expression of genes involved in RA biosynthesis, especially PAL, 4CL1, TAT, and HPPR with up to 4.9-, 3.4-, 4.2-, and 12.7-fold increases, respectively.…”

Section: Discussionmentioning

confidence: 95%

Prunella vulgaris L. hairy roots: Culture, growth, and elicitation by ethephon and salicylic acid

Wang

et al. 2016

Engineering in Life Sciences

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Prunella vulgaris L. hairy roots: Culture, growth, and elicitation by ethephon and salicylic acidHairy roots have gained popularity as an alternative source for secondary metabolites not only because of their biosynthetic and bioactive potentials, but also because of studies of experimental systems aiming to understand secondary metabolic pathways. While many secondary metabolites have been identified in Prunella vulgaris, a comprehensive understanding of the metabolic pathways has remained elusive. Here, Agrobacterium rhizogenes (ATCC15834)-mediated hairy root induction of P. vulgaris resulted in 15-30 times more rosmarinic acid (RA) than in the intact plant. After the growth and RA biosynthesis of P. vulgaris, hairy roots were investigated over a 1-month period, we studied elicitation by ethephon (Eth) and salicylic acid (SA) and found 200 μg L −1 Eth and 6.9 mg L −1 SA exhibited the optimum induction efficiency. These concentrations were further selected for a time-course assay of hairy root cultures to assess exposure to these compounds over 8 days. During that period, RA reached maximum accumulations of 1.66-fold 8 days after Eth elicitation and 1.48-fold 2 days post-SA addition. We also examined the transcripts of genes involved in the RA biosynthesis pathway, and found the expression of tyrosine aminotransferase (TAT) and phenylalanine ammonia-lyase (PAL) were increased by Eth, and SA heightened the expression of TAT, 4-hydroxyphenylpyruvate reductase (HPPR), PAL, 4-coumaric acid CoA-ligase 1 (4CL1), and cytochrome P450-dependent monooxygenase (CYP). Altogether, our results indicate that the convenient hairy root culture system of P. vulgaris can provide an alternate source for RA production and would be conducive for the engineering of secondary metabolic pathways in P. vulgaris.

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Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Cited by 18 publications

References 19 publications

Chitosan Elicitation for Enhancing of Vincristine and Vinblastine Accumulation in Cell Culture of Catharanthus roseus (L.) G. Don

Chitosan Elicitation for Enhancing of Vincristine and Vinblastine Accumulation in Cell Culture of Catharanthus roseus (L.) G. Don

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Prunella vulgaris L. hairy roots: Culture, growth, and elicitation by ethephon and salicylic acid

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