Yeni doğan buzağı ishallerine karşı ticari aşı ile aşılanan sığırlardan doğan yavrularda pasif bağışıklık düzeyi

Abstract

Özet: Bu çalışmada, bir ticari aşı (ScourGuard@(K),Pfizer) ile aşılanan sıgırlarda BRV ve BCV spesifik bagışıklıgın düzeyi ile yeni doganIarın enfeksiyonunda pasif bagışıklıgın rolü incelendi. Bu amaçla gebeliginin 3. trimesterinde bulunan 41 sıgırdan 2 grup oluşturuldu. Grup I'de bulunan sıgırlara ticari aşı prospektüsüne göre aşı uygulandı. Grup II ise kontrol grubu olarak degerlendirildi. Her iki gruptaki sıgırlardan aşı uygulamalanna paralel, dogum yaptıkları gün (L.gün) ve sonraki 3, 7, 14, 21 ve 28. günlerde kan, gaita ve kolostrum/süt (aşı uygulama dönemleri hariç) örnekleri; yeni dogan buzagılardan dogum sonrası 3, 7, 14,21,28. günlerde kan ve gaita örnekleri alındı. Kan ve kolostrumlsüt örnekleri BRV ve BCV spesifik antikorlar yönünden mikronötralizasyon, gaita örnekleri BRV ve BCV antijeni yönünden ELlSA ile kontrol edildi. Elde edilen verilere göre, aşı uygulanan ve aşı uygulanmayan sıgırlara ait kan serumu ve kolostrum/süt örneklerindeki antikor seviyeleri ile yeni dogan buzagıların pasif bagışıklık düzeyi ve enfeksiyona maruz kalma oranları karşılaştırmalı olarak degerlendirildi. Aşı uygulamasını takiben erişkin sıgırlarda aşı etkenlerine spesifik antikor yanıtında belirgin bir artış saptanamadı. Ancak aşı uygulanan ve aşı uygulanmayan annelerden dogan buzagılarda enfeksiyona maruz kalma oranlannın sırasıyla %30.0 ve %54.5 oldugu belirlendi.Anahtar kelimeler: AŞı, coronavirus, maternal bagışıklık, rotavirus, Si gır.Level of the passiye immunity in calves from cows vaccinated for neonatal calf diarrhea Summary: In this study, the level of the BRV and BCV specific immunity in cows which vaccinated with a commercial vaccine (ScourGuard@(K),pfizer) and the role of the passiye immunity in newborns' infections were investigated. For this aim two groups were organised from 4 i cows in their 3 rd trimaster of pregnancy. Group I cows were vaccinated according to the prosedure of the commercial vaccine. Group II cows were used as a control. At the time of the vaccination, at the calving day (l st day) and at 3,7,14,21 and 28 th day s after calving, blood, feces and colostrumlmilk (except vaccination period) samples from cows in each group; from newbom calves at 3,7,14,21,28 lh days after birth blood and feces samples were collected. Blood and colostrumlmilk samples were controlled for the presence of BRV and BCV antibodies using microneutralisation technique. Feces were tested for BRV and BCV antigen by ELlSA. The antibody titers in blood sera and colostrumlmilk from cows, levels of the passiye immunity of newbom calves and the infection rates of calves were evaluated comparatively for Group I and II cows, their calves. Data show n that following the vaccination no remarkable specific antibody increase against vaccine antigens (BRV and BCV) was detected in cows. However the infection rate of calves bom from vaccinated and unvaccinated cows were 30.0% and 54.5%, respectively.Key words: Cattle, coronavirus, maternal immunity, rotavirus, vaccine. GirişYeni doğan buzağı ishallerinde cryptosporidium, en-

A large outbreak of enteritis in goat flocks in Marmara, Turkey, by G8P[1] group A rotaviruses

“…In Turkey, GARs have been associated repeatedly with diarrhea in calves [1,4,30], and GAR-specific antibodies have been demonstrated in the sera of adult bovines [1,4]. In this study, a large outbreak of enteric disease with high morbidity and mortality rates in young kids was associated with GAR infection.…”

“…A South African rotavirus strain, Cap455, was characterised as G6P [14] (GenBank accession numbers AY128708 and AY128709 for the VP7 and VP4, respectively), a VP7/VP4 combination found sporadically in some human and animal rotaviruses [23]. Full-length genome analysis of the G6P [1] strain GO34 isolated in Bangladesh has revealed a mosaic genome composition suggestive of multiple reassortment events with ruminant and human viruses [9]. In addition, by RT-PCR genotyping, two caprine strains from Italy were characterized as G6P [1] [31].…”

“…Full-length genome analysis of the G6P [1] strain GO34 isolated in Bangladesh has revealed a mosaic genome composition suggestive of multiple reassortment events with ruminant and human viruses [9]. In addition, by RT-PCR genotyping, two caprine strains from Italy were characterized as G6P [1] [31]. Accordingly, some caprine viruses display VP4/VP7 antigens common in other ruminant viruses, while some isolates display rare combinations of VP4/VP7 antigens.…”

“…Upon RT-PCR genotyping with a panel of primers specific for various G and P types [5,8,11,12], the isolates were characterized as G8P [1]. Either full-length or partial sequences of the 4 th (VP4), 6 th (VP6), 9 th (VP7) and 10 th (NSP4) genome segments of the Turkish goat rotavirus strain RVA/goat-tc/TUR/Kirklareli/2007 were determined after RT-PCR amplification with specific primer sets [3,5,8,11,12,16,17].…”

“…Identity was 94.6% nt and 98.9% aa in NSP4 and 83.8% nt and 98.5% aa in the VP7. In VP4, the highest identity (83.6% nt and 94.0% aa) was to the goat strain Cap/GO34 and to P [1] viruses of bovine and ovine origin.…”