Identification of rotavirus A strains in small ruminants: first detection of G8P[1] genotypes in sheep in Turkey

Timurkan, Mehmet Ozkan; Alkan, Feray

doi:10.1007/s00705-019-04476-7

Cited by 9 publications

(10 citation statements)

References 38 publications

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“…The only ovine RVA genotype detected in this study was G8P [1]. G8P [1] has been reported as the leading cause of a diarrheal epidemic in two-months-old lambs in Spain [11] and recently was recovered from sheep in Turkey [36]. The genetic combinations G6P [1] and G6P [14] were identified from the caprine samples.…”

Section: Discussionmentioning

confidence: 84%

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Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait

Abdou

Majeed

Saad³

et al. 2021

BMC Vet Res

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Background Group A rotaviruses (RVA) are zoonotic pathogens responsible for acute enteritis in human and neonatal ruminants. This research aimed to determine the prevalence of RVA in ruminants (cattle, sheep, and goats) and investigate the circulating RVA genotypes in these animals in Kuwait. We conducted a cross-sectional study to detect RVA in ruminants, using an immunochromatography test (IC), direct sandwich ELISA test, and real-time RT-PCR (RT-qPCR) assay using fecal samples. Results A total of 400 cattle, 334 sheep, and 222 goats were examined. The prevalence of RVA was 5.3, 1.2, and 2.3%, respectively, using IC. The ELISA test detected RVA from 4.3% of cattle, 0.9% of sheep, and 1.8% of goats. There was a significant association between the occurrence of diarrhea and the presence of RVA in bovine fecal samples (p-value = 0.0022), while no statistical association between diarrhea and the presence of RVA in fecal samples of sheep and goats was observed (p-value = 0.7250; p-value = 0.4499, respectively). Twenty-three of the IC-positive samples (17 from cattle, two from sheep, and four from goats) were tested using a RT-qPCR RVA detection assay targeting the NSP3 gene. The results showed that 21 of 23 IC-positive samples tested positive by RT-qPCR. Detection of RVA genotypes revealed that G10P[11] was the predominant strain in cattle (58.8%), followed by G8P[1] (11.7%). One sheep sample was genotyped as G8P[1]. In addition, G6P[1] and G6P[14] were detected in goat samples. Conclusion The present study revealed that the IC was more sensitive in detecting RVA antigen in fecal samples than the ELISA test. A higher occurrence of RVA infection was observed in cattle than in sheep and goats. This study suggests that RVA might be a risk factor of diarrhea in bovine calves less than 2 weeks old. This research also demonstrates the circulation of RVA in sheep and goat populations in Kuwait. Finally, the G10P[11] RVA genotype was the most prevalent genotype identified from cattle samples.

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Section: Discussionmentioning

confidence: 84%

“…The genetic combinations G6P [1] and G6P [14] were identified from the caprine samples. The G6P [1]genotype combination was the most common genotype reported in goats all over the world (for example, in Turkey [36], Italy [37], and Bangladesh [38]). However, G6P [14] was previously detected from goats in South Africa [39].…”

Section: Discussionmentioning

confidence: 99%

Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait

Abdou

Majeed

Saad³

et al. 2021

BMC Vet Res

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“…All samples included in the study were screened by PCR for canine parvovirus 2 (CPV-2), canine distemper virus, canine adenovirus 1–2, and canine group A rotavirus, using specific primers described previously [ 18 – 21 ]. However, none of the samples were found to be positive for these agents.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of canine coronaviruses: an enteric and pantropic approach

et al. 2020

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Canine coronavirus (CCoV) generally causes an infection with high morbidity and low mortality in dogs. In recent years, studies on coronaviruses have gained a momentum due to coronavirus outbreaks. Mutations in coronaviruses can result in deadly diseases in new hosts (such as SARS-CoV-2) or cause changes in organ-tissue affinity, as occurred with feline infectious peritonitis virus, exacerbating their pathogenesis. In recent studies on different types of CCoV, the pantropic strains characterized by hypervirulent and multi-systemic infections are believed to be emerging, in contrast to classical enteric coronavirus infections. In this study, we investigated emerging hypervirulent and multi-systemic CCoV strains using molecular and bioinformatic analysis, and examined differences between enteric and pantropic CCoV strains at the phylogenetic level. RT-PCR was performed with specific primers to identify the coronavirus M (membrane) and S (spike) genes, and samples were then subjected to DNA sequencing. In phylogenetic analysis, four out of 26 samples were classified as CCoV-1. The remaining 22 samples were all classified as CCoV-2a. In the CCoV-2a group, six samples were in branches close to enteric strains, and 16 samples were in the branches close to pantropic strains. Enteric and pantropic strains were compared by molecular genotyping of CCoV in dogs. Phylogenetic analysis of hypervirulent pantropic strains was carried out at the amino acid and nucleotide sequence levels. CCoV was found to be divergent from the original strain. This implies that some CCoV strains have become pantropic strains that cause multisystemic infections, and they should not be ruled out as the cause of severe diarrhea and multisystemic infections.

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“…cDNA and DNA were used as templates in the PCR stage. For the diagnosis of panpestivirus (BVDV) (p324-326) [3], vesicular stomatitis (VSV) [4], bovine papilloma virus type-2 (BPV-2) (P1-2-3) [5], bovine papular stomatitis virus (BPSV) (PPP1-3-4) [6], foot and mouth disease virus (FMDV) (2BF-2BR) [7], rotavirus (BRV) [8] and coronavirus (BCV) [9] reference primers and PCR protocols were used. The obtained amplicons were analyzed by electrophoresis (110V / 40 min) in 1.5% (w/v) agarose gel stained with ethidium bromide (EtBr).…”

Section: B-c)mentioning

confidence: 99%

Co-Infection of Bovine Papular Stomatitis Virus, Rotavirus and Cryptosporidium Spp. in a Calf

et al. 2020

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Concurrent occurence of bovine papular stomatitis, rotavirus infection and cryptosporidiosis was diagnosed postmortem in a 7-days-old calf from a farm containing 65 calves of different ages. Multifocal papular stomatitis and rumenitis were present on necropsy. While polymerase chain reaction analysis revealed rotavirus and papular stomatitis virus infections; bovine viral diarrhea, foot and mouth disease, bovine papilloma virus and coronavirus could not be detected. Overall; concurrent co-infection with bovine papular stomatitis virus, rotavirus and cryptosporidium spp. was reported for the first time.

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Identification of rotavirus A strains in small ruminants: first detection of G8P[1] genotypes in sheep in Turkey

Cited by 9 publications

References 38 publications

Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait

Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait

Molecular characterization of canine coronaviruses: an enteric and pantropic approach

Co-Infection of Bovine Papular Stomatitis Virus, Rotavirus and Cryptosporidium Spp. in a Calf

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