Yeast ribosomal proteins: XII. YS11 of Saccharomyces cerevisiae is a homologue to E.coli S4 according to the gene analysis

Abstract

We isolated and sequenced a gene, YS11A, encoding ribosomal protein YS11 of Saccharomyces cerevisiae. YS11A is one of two functional copies of the YS11 gene, located on chromosome XVI and transcribed in a lower amount than the other copy which is located on chromosome II. The disruption of YS11A has no effect on the growth of yeast. The 5'-flanking region contains a similar sequence to consensus UASrpg and the T-rich region. The open reading frame is interrupted with an intron located near the 5'-end. The predicted amino acid sequence reveals that yeast YS11 is a homologue to E. coli S4, one of the ram proteins, three chloroplast S4s and others out of the ribosomal protein sequences currently available.

Eukaryotic ribosomal proteins lacking a eubacterial counterpart: important players in ribosomal function

“…The involvement of S27 in ribosome biogenesis is further supported by the finding that, when present in multiple copies, either S27 gene can also suppress the lethality of a ribosome assembly factor RRP7 deletion mutation and can partially restore the ribosomal subunit ratio and pre-rRNA processing step leading to reduced synthesis of the 18S rRNA in Rrp7p-depleted cells (Baudin-Baillieu et al ., 1997). These results prompted the hypothesis that Rrp7p is required for correct assembly of S27 into the preribosomal particle, while the inhibition of pre-rRNA (Petitjean et al ., 1995) P1B a (Remacha et al ., 1995) L3 a (Fried et al ., 1985) P2A a (Remacha et al ., 1995) L4 a (Lucioli et al ., 1988) P2B a (Remacha et al ., 1995) L5 a (Deshmukh et al ., 1995) L12 a (Briones et al ., 1998) L7 a (Arevalo and Warner, 1990) L24 c (Baronas-Lowell and Warner, 1990) L8 b (Yon et al ., 1991) L29 b (DeLabre et al ., 2002) L10 c (Tron et al ., 1995) L39 c (Sachs and Davis, 1990) L11 a (Rotenberg et al ., 1988) L41 b (Yu and Warner, 2001) L18 c (Donovan et al ., 1990) RACK1 b (Chantrel et al ., 1998) L19 c (Song et al ., 1996) L25 a (Rutgers et al ., 1991) L30 c (Dabeva and Warner, 1987) L33 c (Tornow and Santangelo, 1994) L40 b (Finley et al ., 1989) L43 c (Rivlin et al ., 1999) Small subunit S0 a (Montero et al ., 1998) S2 a (All-Robyn et al ., 1990) S4 c (Synetos et al ., 1992) S6 b (Johnson and Warner, 1987) S9 a (Mizuta et al ., 1991) S11 a (Folley and Fox, 1994) S14 a (Paulovich et al ., 1993) S17 b (Abovich et al ., 1985) S19 c (Donovan et al ., 1992) S20 a (Hermann-Le Denmat et al ., 1994) S23 a (Alksne et al ., 1993) processing in Rrp7p-depleted cells appears to be a consequence of a defect in this as...…”

Automatic intron detection in nuclear DNA sequences of Saccharomyces cerevisiae

“…However, the detection of introns in nuclear DNA sequences of yeast is easy, since these introns have precise characteristics permitting an automatic detection. These characteristics are: (a) very well conserved consensus sequences, especially the branchpoint site which is almost invariant (Woolford, 1989;Halfter and Gallwitz, 1988); (b) small intron size (never exceeds 1 kb; Woolford, 1989); (c) in almost all cases, only one intron per gene, with two exceptions: MATa1 (Miller, 1984) and YLSA (Mizuta et al, 1991); (d) location of this unique intron close to the 5' end or in the 5' untranslated region (UTR). All these characteristics permit an automatic detection of yeast nuclear introns by computer.…”

II. Yeast sequencing reports. A 12·5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor

“…YSIlA, located on chromosome XVI (Mizuta et al, 1991). But only the SUP46 copy of the gene is able to complement fully the recessive phenotypes of SUP46 mutations, and YSllA is transcribed at a lower level than SUP46.…”