Yeast engineering to express sex pheromone gland genes of the oriental fruit moth, Grapholita molesta

Vatanparast, Mohammad; Kim, Yonggyun

doi:10.1016/j.aspen.2019.04.009

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…Considering species diversity in moths, desaturases associated with sex pheromone biosynthesis might have undergone a radiating evolution to generate diverse unsaturated fatty acids. Desaturases used for sex pheromone biosynthesis and confirmed by functional assays include Δ5 desaturase [ 10 ], Δ6 desaturase [ 11 ], Δ9 desaturase [ 12 ], Δ10 desaturase [ 13 ], Δ11 desaturase [ 14 ], Δ11/Δ13 dual functional desaturase [ 15 ], Δ14 desaturase [ 16 ], and a terminal desaturase [ 17 ]. Regarding Δ12 desaturase, it has been initially proposed by Jurenka [ 18 ] when assessing sex pheromone biosynthesis of two lepidopteran species ( S .…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

et al. 2021

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Virgin female moths are known to release sex pheromones to attract conspecific males. Accurate sex pheromones are required for their chemical communication. Sex pheromones of Spodoptera exigua, a lepidopteran insect, contain unsaturated fatty acid derivatives having a double bond at the 12th carbon position. A desaturase of S. exigua (SexiDES5) was proposed to have dual functions by forming double bonds at the 11th and 12th carbons to synthesize Z9,E12-tetradecedienoic acid, which could be acetylated to be a main sex pheromone component Z9,E12-tetradecenoic acetate (Z9E12-14:Ac). A deletion of SexiDES5 using CRISPR/Cas9 was generated and inbred to obtain homozygotes. Mutant females could not produce Z9E12-14:Ac along with Z9-14:Ac and Z11-14:Ac. Subsequently, pheromone extract of mutant females did not induce a sensory signal in male antennae. They failed to induce male mating behavior including hair pencil erection and orientation. In the field, these mutant females did not attract any males while control females attracted males. These results indicate that SexiDES5 can catalyze the desaturation at the 11th and 12th positions to produce sex pheromone components in S. exigua. This study also suggests an application of the genome editing technology to insect pest control by generating non-attractive female moths.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

et al. 2021

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“…[77] S. cerevisiae is an ideal host for heterologous gene expression. [86][87][88] The mature technologies of gene editing and expression, high celldensity culture, and fermentation process control made S. cerevisiae to be a very promising microorganism for statins production. The successful synthesis of simvastatin [59] has demonstrated that S. cerevisiae may be a promising microorganism for the production of statins.…”

Section: Challenges and Future Prospectsmentioning

confidence: 99%

Improving statins production: From non‐genetic strategies to genetic strategies

Fan

Tang

Yang

et al. 2023

Biotechnology Journal

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Statins are lipid‐lowering drugs that selectively inhibit 3‐hydroxy‐3‐methylglutaryl coenzyme A (HMG‐CoA) reductase, effectively reducing cholesterol synthesis. With improved nutritional conditions, the demand for statins is increasing in the global market. The use of microbial cell factories for statin biosynthesis has become advantageous due to the rapid advancements in biotechnology. These approaches offer simple operation and easy separation of products. This review provides an overview the strategies for statins production via microbial cell factories, including both traditional fermentation culture (non‐genetic) and modern synthetic biology manufacture (genetic). Firstly, the complex fermentation parameters and process control technology on submerged fermentation (SmF) and solid‐state fermentation (SSF) are introduced in detail. The potential use of recoverable agricultural wastes/(biomass) as a fermentation substrate in SSF for statin production is emphasized. Additionally, metabolic engineering strategies for constructing robust engineering strains and directed evolution are also discussed. The review highlights the potential and challenges of using microbial cell factories for statin production, and aims to promote greener production modes for statins.This article is protected by copyright. All rights reserved

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“…S. cerevisiae is an ideal host for heterologous gene expression (Novo, Bigey et al 2009, Vatanparast and Kim 2019, Davies, Tsyplenkov et al 2021. The mature technologies of gene editing and expression, high celldensity culture and fermentation process control made S. cerevisiae to be a very promising microorganism for statins production.…”

Section: 、、、Challenges and Future Prospectsmentioning

confidence: 99%

Strategies for the statins production in microbial cell factory：present and future

Fan

Tang

Zhao

et al. 2023

Preprint

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Statins as a lipid-lowering drug can selectively inhibit 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and decrease cholesterol synthesis effectively. With the improvement of nutritional conditions, the demand for statins is increasing in global market. Due to the rapid development of modern biotechnologies, the biosynthesis of stains by microbial cell factory appears great advantages. It has the advantages of simple operation and easy separation of products. This review summarized the strategies on statins production via microbial cell factory, including both traditional fermentation culture and modern synthetic biology manufacture. Firstly, the complex fermentation parameters and process control technology on submerged fermentation (SmF) and solid-state fermentation (SSF) were introduced in detail. Especially, the possibility of recoverable agricultural wastes/(Biomass) as fermentation substrate on solid-state fermentation to produce statins was emphasized. Besides, metabolic engineering strategies to construct robust engineering strains and strains evolution were also discussed. The review highlights the potential and challenge of microbial cell factory to yield the statins. Thus, it will facilitate the production of statins in more green production mode.

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Yeast engineering to express sex pheromone gland genes of the oriental fruit moth, Grapholita molesta

Cited by 8 publications

References 44 publications

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pestt

Improving statins production: From non‐genetic strategies to genetic strategies

Strategies for the statins production in microbial cell factory：present and future

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