Yarrowia lipolytica application as a prospective approach for biosynthesis of pyruvic acid from glycerol

Abstract

With the problems related to chemical methods of pyruvic acid (PA) synthesis, a fast-growing interest has been observed in research aiming at reducing the production cost of PA by applying biotechnological methods. This study aimed to investigate the potential applicability of Yarrowia lipolytica Wratislavia 1.31 yeast strain for valorisation of pure and crude glycerol through the production of industrially desired PA. Conditions required for the effective PA biosynthesis, i.e., pH value, thiamine concentration, agitation, and substrate concentration, were examined in batch and fed-batch cultivation modes. The efficient production of PA occurred under the limitation of thiamine (1 µg L −1) and was stimulated by moderate pH (4.5) and agitation (800 rev min −1) of the culture. Under optimal conditions, Y. lipolytica Wratislavia 1.31 was able to produce 85.2 g L −1 of PA with volumetric productivity of 0.90 g L −1 h −1. The yield of PA biosynthesis reached a high level of 1.03 g g −1. Obtained results confirmed the aptitude of Y. lipolytica yeast to produce high amounts of PA from simple glycerol-containing media. Presented process was very promising and might be considered as an attractive alternative for currently used chemical methods of PA synthesis. Keywords Pyruvic acid production • α-ketoglutaric acid • Glycerol • Yarrowia lipolytica • Culture conditions Abbreviations X Biomass (g L −1) PA Pyruvic acid (g L −1) KGA α-ketoglutaric acid (g L −1) Y Pyruvic acid production yield (g g −1) Q Volumetric productivity of pyruvate (g L −1 h −1) q Specific production rate of pyruvic acid (g g −1 h −1) η PA Yield coefficient of PA production

Modelling of the Citric Acid Production from Crude Glycerol by Wild-Type Yarrowia lipolytica DSM 8218 Using Response Surface Methodology (RSM)

“…The concentration of dissolved oxygen (DO) is an important parameter to take in account for the CA production [ 38 ] and generally for the production of organic acids such as isocitric acid [ 15 , 39 ], pyruvic acid [ 40 ] and α-Ketoglutaric Acid [ 41 ]. Ferreira et al [ 42 ] showed that raising the oxygen volumetric mass transfer coefficient (Kla), the conversion of crude glycerol by Y. lipolytica W29 to CA increased of 700%.…”

Low-waste fermentation-derived organic acid production by bipolar membrane electrodialysis—an overview

“…The overall productivity of the process could be enhanced with a coupled-research on membrane development with special regard to the selectivity of ion-exchange membranes. For example, Cybulski et al (Cybulski et al 2018) produced pyruvic acid successfully from glycerol and also obtained significant amounts of α-keto-glutaric acid as a by-product. Both acids are valuable, and their effective separation might be feasible with CED or ED-BPM by the reasoning above.…”