Xenobiotic-induced ribosomal stress compromises dysbiotic gut barrier aging: A one health perspective

Sun, Jiaming; Kim, Juil; Jeong, Hoyoung; Kwon, Dasom; Moon, Yuseok

doi:10.1016/j.redox.2022.102565

Cited by 4 publications

(12 citation statements)

References 69 publications

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“…The procedure was based on the previously published method 68 , 69 . Briefly, kidney sections were stained with PAS, and microphotographs of prepared sections were obtained using an inverted microscope (Nikon-Eclipse Ts2R, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was based on the previously published method 68 , 69 . For gut analysis, the small intestine was immediately removed, fixed in Carnoy’s solution, and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was based on the previously published method 68 , 69 . The prepared tissue sections were deparaffinized in xylene for 10 min, rehydrated in ethanol (100, 90, 80, 70, and 50%) for 3 min each, and then placed in distilled water for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was based on the previously published method 68 , 69 . Frozen tissues were homogenized in 1 mL RiboEx solution (GeneAll Biotechnology, Seoul, Korea) containing stainless-steel beads (5 mm) for 3–6 min using a TissueLyser II (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was based on the previously published method 68 , 69 . Eight-week-old wild-type and Gdf15 KO mice were treated with vehicle or CP (20 mg/kg, intraperitoneally) for 72 h ( n = 4 − 5).…”

Section: Methodsmentioning

confidence: 99%

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Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming

et al. 2023

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The integrated stress response (ISR) plays a pivotal role in the cellular stress response, primarily through global translational arrest and the upregulation of cellular adaptation-linked molecules. Growth differentiation factor 15 (Gdf15) is a potent stress-responsive biomarker of clinical inflammatory and metabolic distress in various types of diseases. Herein, we assess whether ISR-driven cellular stress contributes to pathophysiological outcomes by modulating Gdf15. Clinical transcriptome analysis demonstrates that PKR is positively associated with Gdf15 expression in patients with renal injury. Gdf15 expression is dependent on protein kinase R (PKR)-linked ISR during acute renointestinal distress in mice and genetic ablation of Gdf15 aggravates chemical-induced lesions in renal tissues and the gut barrier. An in-depth evaluation of the gut microbiota indicates that Gdf15 is associated with the abundance of mucin metabolism-linked bacteria and their enzymes. Moreover, stress-responsive Gdf15 facilitates mucin production and cellular survival via the reorganization of the autophagy regulatory network. Collectively, ISR-activated Gdf15 counteracts pathological processes via the protective reprogramming of the autophagic network and microbial community, thereby providing robust predictive biomarkers and interventions against renointestinal distress.

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Section: Methodsmentioning

confidence: 99%

“…The procedure was based on the previously published method 68 , 69 . For gut analysis, the small intestine was immediately removed, fixed in Carnoy’s solution, and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming

et al. 2023

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Nanoplastic contamination: Impact on zebrafish liver metabolism and implications for aquatic environmental health

Rehman,

Huang,

Zhang

et al. 2024

Environment International

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Exogenous Nucleotides Ameliorate Ageing-Related Intestinal Inflammation in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice

et al. 2023

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As one of the most important barriers in the body, the intestinal barrier is a key factor in maintaining human health. Ageing of the intestine is a degenerative process that is closely associated with a variety of poor health conditions in the elderly. Inflammation and the immune system are anti-ageing targets that can regulate the function of the intestine. Nucleotides (NTs) are involved in important physiological and biochemical reactions in the body, but there are few studies about their effect on the ageing intestine. This paper examines the role of exogenous NTs in the ageing intestine. For this purpose, we used senescence-accelerated mouse prone-8 (SAMP8) mice and senescence-accelerated mouse resistant 1 (SAMR1) mice for the experiment, and randomly divided the mice into NTs-free, Normal Control, NTs-low, NTs-medium, NTs-high, and SAMR1 groups. After 9 months of intervention, we collected the colon tissue of mice for testing. In our study, exogenous NTs could increase bodyweight of mice during ageing and improve the morphological structure of the intestine, and we found that NTs could promote the secretion of intestinal protective factors, such as TFF3 and TE. Furthermore, supplementation with NTs suppressed intestinal inflammation and improved intestinal immunity, possibly by activating the p38 signaling pathway. These results suggest that exogenous NTs are able to maintain the health condition of the ageing intestine.

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Xenobiotic-induced ribosomal stress compromises dysbiotic gut barrier aging: A one health perspective

Cited by 4 publications

References 69 publications

Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming

Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming

Nanoplastic contamination: Impact on zebrafish liver metabolism and implications for aquatic environmental health

Exogenous Nucleotides Ameliorate Ageing-Related Intestinal Inflammation in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice

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