Xanthine Oxidase Inhibitory Activity and Antigout of Celery Leek Parsley and Molokhia

Abstract

The present study was aimed at investigating in vitro xanthine oxidase inhibitory (XOI) and in vivo antigout activity extracts of celery, leek, parsley, and molokhia. The degree of XO inhibitory activity was determined by measuring the absorbance spectrophotometrically at 295 nm, which is associated with uric acid formation which is linked to gout. Our preliminary screening study had employed the use of distilled water, and absolute ethanol to determine XOI from celery, leek, parsley, and molokhia. In general, our study showed that the ethanolic extracts were found to be more active than the aqueous extracts. Further in-vivo antigout was studied gout induced in rats by potassium oxonate. A total of 36 male albino rats were randomly divided into 6 equal groups. Group 1 negative control given only standard diet, and group 2-6 given Potassium oxonic acid (250 mg/kg, i.p.), Potassium oxonate an uricase inhibitor was used to induce gout. Oral administration (G3, G4, and G5) of celery, leek, parsley (5 g/Kg), and (G6) molokhia (4.8 g/Kg) showed a significant decrease in uric acid, and Creatinine levels in the gouty rats. All extracts (celery, leek, parsley, and molokhia) have shown significant decrease in level of Malonaldehyde (MDA) and increase in activity of antioxidant enzyme level, comparable to positive rat (G2). No significant changes between all extracts used and negative control in gain weights and organic phosphorus was noticed. The results showed that increasing serum total calcium level with extracts of celery, leek, parsley, and molokhia in comparison to positive control. The celery, leek, parsley, and molokhia extracts have some protective effects on the gout.

Screening medicinal plant extracts for xanthine oxidase inhibitory activity

“…XO inhibitory activity assay is widely used to determine the anti-gout activity of the plant extracts [17]. In this study, XO inhibitory activity of the extract was determined using an in vitro assay with slight modification to the Abd El-Rahman and Abd-ELHak method [18].…”

Synthesis of chiral 3,5-bis(l-phenylalaninyl-l-leucinyl)pyridine Schiff base and their macrocyclic carboxaimide derivatives using 3,5-bis(l-phenylalaninyl)-pyridine methyl ester

“…A comparison of our results with previous results reported for antimicrobial activities of oligopeptides shows that our synthesized derivatives have potential antimicrobial activities against tested pathogens. Abd El Rahman et al [30] obtained their highest antimicrobial activities (13.1 mm against B. subtilis) for their synthesized peptide (PEG-Gly-Tyr-Tyr-Ala-NH 2 ) at their used concentration of 10 mg mL −1 , while this peptide was not active at all against S. aureus and E. coli. It is noteworthy to mention that our results were obtained at the 50 μg mL −1 level.…”

Untitled

“…The xanthine oxidase inhibitory activity was measured spectrophotometrically at 290 nm in 96 well plates, with some modification (15). The extracts were initially dissolved in dimethyl sulfoxide (DMSO), before being dissolved in phosphate buffer (pH.…”