The present study was aimed at investigating in vitro xanthine oxidase inhibitory (XOI) and in vivo antigout activity extracts of celery, leek, parsley, and molokhia. The degree of XO inhibitory activity was determined by measuring the absorbance spectrophotometrically at 295 nm, which is associated with uric acid formation which is linked to gout. Our preliminary screening study had employed the use of distilled water, and absolute ethanol to determine XOI from celery, leek, parsley, and molokhia. In general, our study showed that the ethanolic extracts were found to be more active than the aqueous extracts. Further in-vivo antigout was studied gout induced in rats by potassium oxonate. A total of 36 male albino rats were randomly divided into 6 equal groups. Group 1 negative control given only standard diet, and group 2-6 given Potassium oxonic acid (250 mg/kg, i.p.), Potassium oxonate an uricase inhibitor was used to induce gout. Oral administration (G3, G4, and G5) of celery, leek, parsley (5 g/Kg), and (G6) molokhia (4.8 g/Kg) showed a significant decrease in uric acid, and Creatinine levels in the gouty rats. All extracts (celery, leek, parsley, and molokhia) have shown significant decrease in level of Malonaldehyde (MDA) and increase in activity of antioxidant enzyme level, comparable to positive rat (G2). No significant changes between all extracts used and negative control in gain weights and organic phosphorus was noticed. The results showed that increasing serum total calcium level with extracts of celery, leek, parsley, and molokhia in comparison to positive control. The celery, leek, parsley, and molokhia extracts have some protective effects on the gout.

The content of Cd, Pb, Al, and As in fresh vegetables, green salads and potato fried purchased from the Egyptian market and restaurant were determined using atomic absorption spectrometry (AAS).The results of this study showed that there was wide variation in the concentration of these metals in vegetables collected from different sites. The highest Cd level in watercress (8.33 mg/Kg) was found in Cairo. Vegetables grown in Cairo and Alexandria had cadmium many folds higher than those of other towns. Lead concentration in potato of Alexandria is more than permitted level. Aluminium (Al) content was high concentration in all vegetable samples and arsenic (As) concentration mostly was appeared in potato. Analytical results indicated that the concentration of Cd, Pb, and As in green salad samples were so few. The highest concentration of Al was detected in salad collected from popular restaurant 8. The results show that the heavy metal content in unprocessed potato appears to be much higher than that in processed potato. The highest levels of Cd, Pb, and Al were detected in popular restaurant 9. Cd and As contents of international chain restaurant 1 were found in high level (2.958 and 0.95mg/Kg).

Shigellosis causes severe diarrhea and, in some cases, death in children. In this study, we investigated the in vitro and in vivo anti‐shigellosis efficacy of some aqueous herbal extracts or their chosen mixtures, including fenugreek seed, black seed, clove, garlic, and ginger. The antibacterial potential was assessed by well diffusion and microdilution assays. The extracts' minimum inhibitory concentrations ranged from 0.39 to 12.5 mg/ml. The water extract of garlic and combinations of garlic with either fenugreek or clove extracts had the highest antibacterial efficacy, hence these extracts were chosen for the in vivo investigation. Rats were given a Shigella dysenteriae inoculum and then administrated two doses (100 and 200 mg/kg bw) of the selected herbal extracts or the antibiotic ciprofloxacin (20 mg/kg bw) to treat Shigellosis in vivo. The results showed that garlic and clove extract mixtures (G6, 200 mg/kg bw), garlic and fenugreek mixtures (G7 and G8, 100 and 200 mg/kg bw), and ciprofloxacin group (G9, 20 mg/kg bw) had the best anti‐Shigellosis efficacy. G7 (garlic and fenugreek mixtures (100 mg/kg bw) outperformed almost all other groups evaluated. This group had lower defecation frequency, lower stool‐Shigella loudness, restored body weight, recovered hematological, and liver and kidney functions, besides exhibited no anatomical alterations in the colon tissue that were comparable to the uninfected group. As a result, in the treatment of Shigella, this extract combination may be suggested as a viable therapeutic alternative to antibiotics.