WS2 and MoS2 biosensing platforms using peptides as probe biomolecules

Abstract

Biosensors based on the two-dimensional layered nanomaterials transition metal dichalcogenides such as WS2 and MoS2 have shown broad applications, while they largely rely on the utilization of single stranded DNA as probe biomolecules. Herein we have constructed novel WS2- and MoS2- based biosensing platforms using peptides as probe biomolecules. We have revealed for the first time that the WS2 and MoS2 nanosheets display a distinct adsorption for Arg amino acid and particularly, Arg-rich peptdies. We have demonstrated that the WS2 and MoS2 dramatically quench the fluorescence of our constructed Arg-rich probe peptide, while the hybridization of the probe peptide with its target collagen sequence leads to the fluorescence recovery. The WS2-based platform provides a sensitive fluorescence-enhanced assay that is highly specific to the target collagen peptide with little interferences from other proteins. This assay can be applied for quantitative detection of collagen biomarkers in complex biological fluids. The successful development of WS2- and MoS2- based biosensors using non-ssDNA probes opens great opportunities for the construction of novel multifunctional biosensing platforms, which may have great potential in a wide range of biomedical field.

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

“…The sensing interface ability of present genosensor was compared with earlier reported MoS 2 based biosensors 37–52 . High sensitivity, specificity and repeatability of the sensor make it best among other biosensors (Supplementary Table 1).…”

Impedance Biosensing atop MoS2 Thin Films with Mo−S Bond Formation to Antibody Fragments Created by Disulphide Bond Reduction

“…The majority of methods reported for biomolecule immobilization onto MoS 2 involve physisorption , which suffers from problems with stability and leaching of immobilized species during sensor storage and use. Only a few studies report covalent immobilization of biomolecules atop MoS 2 . These include reports of Ni 2+ adsorption followed by protein binding through a polyhistidine tag , direct binding of carboxylate groups to MoS 2 followed by amide bond formation to proteins , and bond formation between a thiol group on the biomolecule and MoS 2 , as reported here.…”

“…Only a few studies report covalent immobilization of biomolecules atop MoS 2 . These include reports of Ni 2+ adsorption followed by protein binding through a polyhistidine tag , direct binding of carboxylate groups to MoS 2 followed by amide bond formation to proteins , and bond formation between a thiol group on the biomolecule and MoS 2 , as reported here. All studies of covalent bond formation between MoS 2 and biomolecules involve nanosheets of MoS 2 that are subsequently attached to another substrate material .…”

“…However, most studies report biomolecular physisorption atop MoS 2 . Only a few studies report chemisorption atop MoS 2 , and these studies utilize nanosheets of MoS 2 that are subsequently attached to another substrate material . Here we report alternative methods for biomolecular chemisorption atop electrodeposited MoS 2 thin films that can be used directly for biosensing.…”

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

“…Apart from MoS 2 , WS 2 could also act as an efficient quencher for fluorescence donors [85,[111][112][113][114][115][116][117][118][119][120]. Xi et al [111] reported for the first time that the WS 2 nanosheets were utilized to develop a FRET sensor for the detection of microRNA.…”