Will advances in fish immunology change vaccination strategies?

Secombes, Chris J.

doi:10.1016/j.fsi.2008.05.001

Cited by 84 publications

(53 citation statements)

References 86 publications

(95 reference statements)

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“…In this scenario, controlling disease outbreaks by vaccination and the use of immune modulator enriched diets represents a major achievement for aquaculture species. Although fish vaccination is still crawling, it has been successfully used to control important fish diseases (Sommerset et al 2005, Secombes 2008, Van Muiswinkel 2008, Plant & Lapatra 2011. And, more recently, vaccination commingled with the inclusion of immune-modulator molecules in the diet offered and additional strategy to overcome major pathogen (Newaj-Fyzul & Austin 2015).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Domenico

Canova²,

Soveral³

et al. 2017

Pesq. Vet. Bras.

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RESUMO.-[Efeito imunomodulador da β-glucana usada como aditivo alimentar em jundiás (Rhamdia quelen).]O uso da β-glucana como suplemento alimentar foi avaliado em jundiás. A β-glucana foi adicionada à ração na proporção de 0.01%, e 0.1% e fornecida aos peixes por 21, para avaliar dados hematológicos e parâmetros do sistema imune natural, ou 42 dias, para avaliar ganho de peso e resistência ao desafio com Aeromonas hydrophila. A adição da β-glucana na dieta não afetou o ganho de peso e não induziu alterações hematológicas nem alterações nos níveis de aglutininas e mieloperoxidase sanguínea. No entanto, a atividade hemolítica natural do sistema do complemento foi significativamente maior nos peixes alimentados com β-glucana. Além disso, nos peixes alimentados com β-glucana e desafiados com A. hydrophila, o número de bactérias isoladas do sangue foi significativamente menor, e a sobrevivência ao desafio foi significativamente maior do que nos peixes que não receberam β-glucana. Consequentemente, concluímos que a β-glucana tem potencial imunomodulador quando adicionada à dieta, nas condições experimentais aqui indicadas, e contribui para aumentar imunidade natural e a resistência dos jundiás ao desafio com patógenos específicos. INTRODUCTIONThe occurrence of infectious diseases is one of the major causes of losses in modern aquaculture (Sitjà-Bobadilla The immunomodulatory effects of dietary β-glucan were evaluated in silver catfish. β-glucan was added to the diet (0.01%, and 0.1%) and fed to the fish for 21 days, to evaluate effects on blood and some innate immune parameter, or fed for 42 days, to evaluate growth rate and resistance to challenge with pathogenic Aeromonas hydrophila. We found that adding β-glucan to the diet had no effect on fish growth and no effect on blood cells, or serum bacterial agglutination and serum myeloperoxidase activity. However, fish that received β-glucan in the diet had the natural hemolytic activity of complement significantly higher compared to control fish. Furthermore, fish fed with β-glucan and challenged with A. hydrophila had fewer bacteria in blood and presented a significantly higher survival rate compared to control fish. Thus, we concluded that β-glucan might be explored as feed additive aiming to improve silver catfish innate immunity and resistance to specific pathogen.INDEX TERMS: Silver catfish, Rhamdia quelen, fish, immunostimulants, β-glucan, Aeromonas hydrophila.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Domenico

Canova²,

Soveral³

et al. 2017

Pesq. Vet. Bras.

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“…The head kidney in teleosts is a major site of lymphoid tissue. It produces mainly macrophages and lymphoid cells, and is important for the capture of antigens and the production of antibodies (Secombes 2008). TNF-α is an important early response pro-inflammatory cytokine, and the level of its expression can indicate the presence of an inflammatory response (Press and Evensen 1999).…”

Section: Discussionmentioning

confidence: 99%

Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss)

Matejová¹,

Vícenová²,

Vojtek³

et al. 2015

Vet. Med.

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ABSTRACT:The trichothecene mycotoxin deoxynivalenol (DON) is commonly found as a natural contaminant in cereals such as wheat, barley, and corn, and exhibits various toxicological effects when present in animal feeds. The effects of DON at a nominal 2 mg/kg feed on immune responses of rainbow trout were investigated, including relative gene expression of important cytokines (TNF-α, IL-8, IL-1β, IL-10), lysozyme concentration in skin mucus, and level of antigen-specific IgM in blood plasma after vaccination with the commercial vaccine AquaVac ERM containing Yersinia ruckeri type 1 (Hagerman strain). Twenty one-year-old rainbow trout Oncorhynchus mykiss were randomly divided into two groups. The control received a commercial feed with a naturally occurring low level of DON (225 μg/kg feed), while an experimental group was fed the same formulation with DON added to 1964 μg/kg feed. The trial continued for 23 days. Consumption of feed with added DON showed a significant effect on the immune system, as indicated by a higher level of pro-inflammatory cytokine TNF-α (P < 0.05) and of IL-8 (non-significant) in head kidney. Expression of the pro-inflammatory gene IL-1β and the expression of a gene encoding anti-inflammatory cytokine (IL-10) were not influenced by DON treatment. Effects on the concentration of skin mucus lysozyme and specific IgM antibody levels were not observed during this experiment. These results suggest that prolonged ingestion of low doses of DON may influence the immune responses of rainbow trout.

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“…However, the continuous growth of fish husbandry and intensive production systems comes along with new challenges like outbreaks of infectious diseases and the need to monitor exposure to microorganism (Secombes 2008). In this scenario, knowledge of effective defense mechanisms and developing immunobiological reagents for immunoassays are much needed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Kreutz

Canova²,

Nied³

et al. 2016

Pesq. Vet. Bras.

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Knowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species.

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Will advances in fish immunology change vaccination strategies?

Cited by 84 publications

References 86 publications

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss)

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

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