Why do exopolysaccharide yields from the fungus Aureobasidium pullulans fall during batch culture fermentation?

Campbell, Bradley S.; McDougall, Barbara M.; Seviour, Robert J.

doi:10.1016/s0141-0229(03)00089-9

Cited by 23 publications

(35 citation statements)

References 40 publications

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“…Whether it also liberates cell wall bound β-glucanases (Martin et al, 2007) capable of degrading these exocellular β-glucans in fungi where yields fall in the later stages of the fermentation (Rapp, 1989;Stahmann et al, 1992) is worth pursuing. Increases in enzymatic activity often coincide with glucose exhaustion from the medium, as reported for A. pullulans too (Campbell et al, 2003;Orr et al, 2009;Zheng et al, 2008), which implies these exocellular glucans may have a role as carbon and energy reserves in the producing fungi. Yet not all glucan producing fungi seem to synthesize β-glucanases (Martin et al, 2007).…”

Section: Branched Fungal (13) (16)-β-glucansmentioning

confidence: 54%

Operating bioreactors for microbial exopolysaccharide production

Seviour¹,

McNeil²,

Fazenda³

et al. 2010

Critical Reviews in Biotechnology

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There is considerable interest in exploiting the novel physical and biological properties of microbial exopolysaccharides in industry and medicine. For economic and scientific reasons, large scale production under carefully monitored and controlled conditions is required. Producing exopolysaccharides in industrial fermenters poses several complex bioengineering and microbiological challenges relating primarily to the very high viscosities of such culture media, which are often exacerbated by the producing organism's morphology. What these problems are, and the strategies for dealing with them are discussed critically in this review, using pullulan, curdlan, xanthan, and fungal β-glucans as examples of industrially produced microbial exopolysaccharides. The role of fermenter configuration in their production is also examined.

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Section: Branched Fungal (13) (16)-β-glucansmentioning

confidence: 54%

Operating bioreactors for microbial exopolysaccharide production

Seviour¹,

McNeil²,

Fazenda³

et al. 2010

Critical Reviews in Biotechnology

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show abstract

“…This study also concluded that EPS production kinetics by A. pullulans was dependent on the specific microorganism-medium-environment system. Campbell, McDougall, and Seviour (2003); Orr et al (2009) have shown that various concentrations of NaNO 3 affected the EPS formation by A.…”

Section: Effect Of Nitrogen Sourcesmentioning

confidence: 99%

Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Rao¹,

Quiñones

Retter³

et al. 2010

Carbohydrate Polymers

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“…The results indicated that, with similar rates of carbon utilization, the diversion of glucose from incorporation into cellular material to the elaboration of polysaccharide is dependent on ammonium ion concentration. Campbell et al (2003) also reported that NH þ 4 might exert its influence as an effector of pullulan-degrading enzyme activity controlling carbon flow within the cell. Additionally, excess nitrogen supply could contribute to the higher level of biomass, but not enhanced polysaccharide production (Orr et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…However, this phenomenon is only the consequence of the exhaustion of the carbon source and with the high initial nitrogen concentration (Campbell et al, 2003). Therefore, pullulan-degrading enzyme activity can also be used as an indicator to explain the pullulan production profiles.…”

Section: Introductionmentioning

confidence: 99%