Effects of initial ammonium ion concentration on pullulan production by Aureobasidium pullulans and its modeling

Cheng, Kuan‐Chen; Demirci, Ali; Catchmark, Jeffrey M.; Puri, Virendra M.

doi:10.1016/j.jfoodeng.2010.10.004

Cited by 30 publications

(11 citation statements)

References 27 publications

(28 reference statements)

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“…When A. pullulans CGMCC1234 was grown in the medium containing 0.5 % Tween 80, 8.0 % sucrose, and 0.09 % yeast extract, 55.5 g/l of pullulan was accumulated in the fermented medium (Shen et al 2013). A. pullulans produced the highest pullulan (23.1 g/l) when the initial ammonium sulfate was at 0.5 % (Cheng et al 2011). A. pullulans CJ001 isolated from sea mud produced 31.25 g/l of melanin-free pullulan ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin

Liu

Chi

et al. 2015

Mar Biotechnol

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In this study, in order to directly and efficiently convert inulin into pullulan, the INU1 gene from Kluyveromyces maximum KM was integrated into the genomic DNA and actively expressed in the high pullulan producer Aureobasidium melanogenum P16 isolated from the mangrove ecosystem. After the ability to produce pullulan from inulin by different transformants was examined, it was found that the recombinant strain EI36, one of the transformants, produced 40.92 U/ml of inulinase activity while its wild-type strain P16 only yielded 7.57 U/ml of inulinase activity. Most (99.27 %) of the inulinase produced by the recombinant strain EI36 was secreted into the culture. During the 10-l fermentation, 70.57 ± 1.3 g/l of pullulan in the fermented medium was attained from inulin (138.0 g/l) within 108 h, high inulinase activity (42.03 U/ml) was produced within 60 h, the added inulin was actively hydrolyzed by the secreted inulinase, and most of the reducing sugars were used by the recombinant strain EI36. This confirmed that the genetically engineered yeast of A. melanogenum strain P16 was suitable for direct pullulan production from inulin.

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Section: Discussionmentioning

confidence: 99%

Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin

Liu

Chi

et al. 2015

Mar Biotechnol

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“…Pullulan‐degrading enzyme activity was detected by Cheng et al . under sucrose depletion and resulted in a concomitant decrease in pullulan production.…”

Section: Resultsmentioning

confidence: 99%

“…400 rpm) after the first culture stage seems more favorable to prevent over‐degrading of pullulan, which in turn would help to maintain a higher average Mw of pullulan. Our findings indicate that pullulan‐degrading activity is already available before carbon source depletion, which is not in full agreement with the results reported by Cheng et al . Accordingly, a higher yield and higher Mw of pullulan could be expected if the DO supply was properly controlled during the batch process.…”

Section: Resultsmentioning

confidence: 99%

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Simultaneously enhanced production and molecular weight of pullulan using a two‐stage agitation speed control strategy

Wang

Bian

Wei

et al. 2014

J of Chemical Tech & Biotech

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BACKGROUND Pullulan with high molecular weight (Mw) is attractive for industrial application. Previous studies indicated that high yield and high Mw of pullulan cannot be simultaneously obtained under constant agitation speeds or constant dissolved oxygen levels during batch pullulan fermentation. In this study, a two‐stage agitation speed control strategy was developed to obtain pullulan with both high yield and high Mw. RESULTS Batch pullulan production at various constant agitation speeds ranging from 200 to 600 rpm was carried out in a 5 L stirred tank fermentor. Based on kinetic analysis of batch processes, activity assays of the enzymes associated with pullulan biosynthesis and biodegradation, and intracellular energy supply under different constant agitation speeds, a two‐stage control strategy that shifted the agitation speed from 500 to 400 rpm at 18 h proved to effectively achieve simultaneous high yield of high Mw pullulan. CONCLUSION A maximum pullulan concentration of 27.4 g L−1 and final Mw of 3.09 × 106 Da were obtained using the two‐stage agitation speed control strategy, which were both greatly improved compared with those obtained at constant agitation speeds. © 2014 Society of Chemical Industry

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“…Several reports suggested that type of nitrogenous substrate and its concentration in the medium crucially affected the yield and structure of EPSs produced by A. pullulans [19][20][21][22] . When both NRRL 58539 and NRRL 58543 were individually grown in a culture media containing either ammonium or nitrate at different concentrations, EPS with appearance similar to pullulan had not been detected (data not shown).…”

Section: Discussionmentioning

confidence: 99%

An aubasidan-like β-glucan produced by Aureobasidium pullulans in Thailand

Unhapattaratitikul

Seelanan²,

Punnapayak³

2013

ScienceAsia

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ABSTRACT:The nutrient assimilation profile of two non-pullulan-producing strains of Aureobasidium pullulans (NRRL 58539 and NRRL 58543) isolated in Thailand was investigated. Similar nutrient assimilation was observed between both strains and the pullulan-producing A. pullulans NRRL 58560. All three strains weakly assimilated methyl-α-D-glucoside and lactose. For the exopolysaccharide (EPS) production, NRRL 58539 and NRRL 58543 preferred NaNO3 as the sole nitrogen source. Enzyme sensitivity assays, infrared spectroscopy, and nuclear magnetic resonance analyses of the EPSs revealed a broad structural similarity to aubasidan and not to pullulan. This is the first report of an aubasidan-like β-glucan produced by A. pullulans in Thailand.

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Effects of initial ammonium ion concentration on pullulan production by Aureobasidium pullulans and its modeling

Cited by 30 publications

References 27 publications

Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin

Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin

Simultaneously enhanced production and molecular weight of pullulan using a two‐stage agitation speed control strategy

An aubasidan-like β-glucan produced by Aureobasidium pullulans in Thailand

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