Whole-plant corn silage improves rumen fermentation and growth performance of beef cattle by altering rumen microbiota

Cui, Yalei; Liu, Hua; Gao, Zimin; Xu, Junying; Liu, Boshuai; Guo, Ming; Yang, Xu; Niu, Jiakuan; Zhu, Xiaoyan; Ma, Sen; Li, Defeng; Sun, Yu; Ya, Shi

doi:10.1007/s00253-022-11956-5

Cited by 26 publications

(25 citation statements)

References 56 publications

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“…The PCR products were extracted from a 2% agarose gel and further purified using an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City, CA, USA). Three independent PCRs from each sample were conducted, and the PCR products were mixed and sequenced as a single sample ( 59 ). A TruSeqIM DNA sample prep kit was used to build the library, and the MiSeq PE300 platform of the Illumina Company was used for sequencing.…”

Section: Methodsmentioning

confidence: 99%

Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Niu

Liu

et al. 2023

Microbiol Spectr

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Section: Methodsmentioning

confidence: 99%

Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Niu

Liu

et al. 2023

Microbiol Spectr

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“…In the present study, bacterial ASV richness was higher in GSG samples than in SG samples, further supporting that a concentrated diet can reduce bacterial diversity (McCann et al, 2014;Ku et al, 2021), while fungal richness and diversity were higher in the NSG and SG rumen. As cellulose and hemicellulose-degrading bacteria (Zened et al, 2013;Sha et al, 2020;Cui et al, 2022), Succinivibrionaceae_UCG-002 and Rikenellaceae_RC9_gut_group were enriched in GSG rumen, which had an intermediate fiber content (NSG > GSG > SG). At the species level, R. albus was more abundant in GSG rumen than that in SG rumen, while F. succinogenes, B. fibrisolvens, and R. flavefaciens were more abundant in SG samples than NSG.…”

Section: Discussionmentioning

confidence: 99%

Simulated seasonal diets alter yak rumen microbiota structure and metabolic function

et al. 2022

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Yak is the only ruminant on the Qinghai-Tibetan Plateau that grazes year-round. Although previous research has shown that yak rumen microbiota fluctuates in robust patterns with seasonal foraging, it remains unclear whether these dynamic shifts are driven by changes in environment or nutrient availability. The study examines the response of yak rumen microbiota (bacteria, fungi, and archaea) to simulated seasonal diets, excluding the contribution of environmental factors. A total of 18 adult male yaks were randomly divided into three groups, including a nutrition stress group (NSG, simulating winter pasture), a grazing simulation group (GSG, simulating warm season pasture), and a supplementation group (SG, simulating winter pasture supplemented with feed concentrates). Volatile fatty acids (VFAs) profiling showed that ruminal acetate, propionate and total VFA contents were significantly higher (p < 0.05) in GSG rumen. Metagenomic analysis showed that Bacteroidetes (53.9%) and Firmicutes (37.1%) were the dominant bacterial phyla in yak rumen across dietary treatments. In GSG samples, Actinobacteriota, Succinivibrionaceae_UCG-002, and Ruminococcus albus were the most abundant, while Bacteroides was significantly more abundant in NSG samples (p < 0.05) than that in GSG. The known fiber-degrading fungus, Neocallimastix, was significantly more abundant in NSG and SG samples, while Cyllamyces were more prevalent in NSG rumen than in the SG rumen. These findings imply that a diverse consortium of microbes may cooperate in response to fluctuating nutrient availability, with depletion of known rumen taxa under nutrient deficiency. Archaeal community composition showed less variation between treatments than bacterial and fungal communities. Additionally, Orpinomyces was significantly positively correlated with acetate levels, both of which are prevalent in GSG compared with other groups. Correlation analysis between microbial taxa and VFA production or between specific rumen microbes further illustrated a collective response to nutrient availability by gut microbiota and rumen VFA metabolism. PICRUSt and FUNGuild functional prediction analysis indicated fluctuation response of the function of microbial communities among groups. These results provide a framework for understanding how microbiota participate in seasonal adaptations to forage availability in high-altitude ruminants, and form a basis for future development of probiotic supplements to enhance nutrient utilization in livestock.

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“…The production of SCFA is important in energy homeostasis ( Schwiertz et al, 2010 ; Blaut, 2015 ). In addition, ultra-early weaning led to an increase in intestinal Prevotellaceae_UCG-003 , which is closely related to polysaccharide, protein, energy, and vitamin metabolism ( Cui et al, 2022 ), and a decrease in Desulfovibrio and Christensenellaceae_R.7_group , which are the bacteria involved in inducing bowel disease ( Marini et al, 2002 ; Mancabelli et al, 2017 ). On day 70 of age, ultra-early weaning led to an increase in Bacteroides ( Yang et al, 2018 ) and Phascolarctobacterium ( Yang et al, 2018 ), which are related to the production of SCFA, as well as Prevotellaceae_UCG-003 , which is related to polysaccharide, protein, energy, and vitamin metabolism ( Cui et al, 2022 ), but a decrease in Prevotella , which is involved in infections ( Giri and Mangalam, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, ultra-early weaning led to an increase in intestinal Prevotellaceae_UCG-003 , which is closely related to polysaccharide, protein, energy, and vitamin metabolism ( Cui et al, 2022 ), and a decrease in Desulfovibrio and Christensenellaceae_R.7_group , which are the bacteria involved in inducing bowel disease ( Marini et al, 2002 ; Mancabelli et al, 2017 ). On day 70 of age, ultra-early weaning led to an increase in Bacteroides ( Yang et al, 2018 ) and Phascolarctobacterium ( Yang et al, 2018 ), which are related to the production of SCFA, as well as Prevotellaceae_UCG-003 , which is related to polysaccharide, protein, energy, and vitamin metabolism ( Cui et al, 2022 ), but a decrease in Prevotella , which is involved in infections ( Giri and Mangalam, 2019 ). Therefore, ultra-early weaning seems to increase beneficial bacteria and decrease harmful bacteria in the intestine and thus benefit establishing a healthy intestinal microbiota during the early life of piglets, which was affirmed by the study of Smith et al (2008) ; shortening suckling period would decrease the risk of the vertical transmission of pathogens from sows to offspring.…”

Section: Discussionmentioning

confidence: 99%

Ultra-early weaning alters growth performance, hematology parameters, and fecal microbiota in piglets with same genetic background

Dang

et al. 2022

Front. Microbiol.

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Piglets with the same genetic background were used to investigate the effects of different lengths of suckling period on growth performance, hematology parameters, and fecal microbiota. All piglets were born by a sow (Landrace×Yorkshire). On day 14 postpartum, a total of 16 piglets [Duroc×(Landrace×Yorkshire)] with a similar initial body weight (2.48 ± 0.25 kg) were randomly assigned into two groups with four replicates per group, two pigs per replicate pen (one barrow and one gilt). On day 14 of age, experiment started, piglets from the first group were weaned (14W), whereas the others continued to receive milk until day 28 of age (28W). The experiment completed on day 70 of age, last 56 days. Growth performance parameters including body weight, average daily gain, feed intake, feed efficiency, and growth rate and hematology parameters including immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), albumin, globulin, and total protein were measured in this study. Additionally, a technique of 16S rRNA gene sequencing was used to analyze fecal microbiota for revealing how the changes in the lengths of suckling period on intestinal microbiota. We found that ultra-early weaning impaired growth performance of piglets, whose worse body weight, average daily gain, feed intake, feed efficiency, and growth rate were observed in 14W group at all measured timepoints in comparison with those in 28W group (P < 0.05). Moreover, higher contents of serum IgA (P = 0.028), IgG (P = 0.041), and IgM (P = 0.047), as well as lower contents of serum albumin (P = 0.002), albumin-to-globulin ratio (P = 0.003), and total protein (P = 0.004), were observed in 14W group in comparison with those in 28W group on day 28 of age, but not on day 70 of age. High-throughput pyrosequencing of 16S rRNA indicated that the intestinal microbiota richness in 14W group was lower than that in 28W group (P < 0.05); moreover, in comparison with 28W group at all sampling timepoints, fecal microbiota in 14W group showed more beneficial bacteria and fewer pathogenic bacteria (P < 0.05). Therefore, we considered that ultra-early weaning had positive effects on immune status and fecal microbiota composition in piglets, but negative effects on growth performance and fecal microbiota abundance.

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Whole-plant corn silage improves rumen fermentation and growth performance of beef cattle by altering rumen microbiota

Cited by 26 publications

References 56 publications

Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Simulated seasonal diets alter yak rumen microbiota structure and metabolic function

Ultra-early weaning alters growth performance, hematology parameters, and fecal microbiota in piglets with same genetic background

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