Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Niu, Jiakuan; Liu, Xiao; Xu, Junying; Li, Fen; Wang, Jincan; Zhang, Xixi; Yang, Xu; Wang, Lin; Ma, Sen; Li, Defeng; Zhu, Xiaoyan; Wang, Chengzhang; Ya, Shi; Cui, Yalei

doi:10.1128/spectrum.02416-22

Cited by 20 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These four genera were restored after the MK-7 supplementation (all P < 0.05, Additional file 1 : Fig.S6A). Flexilinea belongs to the methanogens, which have been reported to be positively correlated with the increase of blood glucose and blood lipid parameters [ 33 ]; Escherichia-Shigella are the potentially pathogenic bacteria which have been correlated with the dysbiosis of microbiota [ 34 ]; and Lachnospiraceae are the family subset of the phylum Firmicutes which are known to be responsible for the production of SCFA [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, Clostridiales , Ruminococcaceae , Lachnospiraceae , and Eubacterium_coprostanoligenes_group are capable of bile acid 7alpha-dehydroxylation to produce SBAs, and these taxa were also showed an increasing trend after MK-7 intervention (Fig. 2 D, Additional file 1 : Fig.S3C, Fig.S5F) [ 35 , 58 – 61 ]. To the best of our knowledge, this is the first study to discover the association between MK-7 and increased synthesis of SBAs or SCFAs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin K2 supplementation improves impaired glycemic homeostasis and insulin sensitivity for type 2 diabetes through gut microbiome and fecal metabolites

Zhang

Liu

Wei

et al. 2023

BMC Med

View full text Add to dashboard Cite

Background There is insufficient evidence for the ability of vitamin K2 to improve type 2 diabetes mellitus symptoms by regulating gut microbial composition. Herein, we aimed to demonstrate the key role of the gut microbiota in the improvement of impaired glycemic homeostasis and insulin sensitivity by vitamin K2 intervention. Methods We first performed a 6-month RCT on 60 T2DM participants with or without MK-7 (a natural form of vitamin K2) intervention. In addition, we conducted a transplantation of the MK-7-regulated microbiota in diet-induced obesity mice for 4 weeks. 16S rRNA sequencing, fecal metabolomics, and transcriptomics in both study phases were used to clarify the potential mechanism. Results After MK-7 intervention, we observed notable 13.4%, 28.3%, and 7.4% reductions in fasting serum glucose (P = 0.048), insulin (P = 0.005), and HbA1c levels (P = 0.019) in type 2 diabetes participants and significant glucose tolerance improvement in diet-induced obesity mice (P = 0.005). Moreover, increased concentrations of secondary bile acids (lithocholic and taurodeoxycholic acid) and short-chain fatty acids (acetic acid, butyric acid, and valeric acid) were found in human and mouse feces accompanied by an increased abundance of the genera that are responsible for the biosynthesis of these metabolites. Finally, we found that 4 weeks of fecal microbiota transplantation significantly improved glucose tolerance in diet-induced obesity mice by activating colon bile acid receptors, improving host immune-inflammatory responses, and increasing circulating GLP-1 concentrations. Conclusions Our gut-derived findings provide evidence for a regulatory role of vitamin K2 on glycemic homeostasis, which may further facilitate the clinical implementation of vitamin K2 intervention for diabetes management. Trial registration The study was registered at https://www.chictr.org.cn (ChiCTR1800019663).

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vitamin K2 supplementation improves impaired glycemic homeostasis and insulin sensitivity for type 2 diabetes through gut microbiome and fecal metabolites

Zhang

Liu

Wei

et al. 2023

BMC Med

View full text Add to dashboard Cite

show abstract

“…Fermented mulberry leaves may also regulate lipid metabolism by regulating the intestinal flora and optimising the composition of short‐chain fatty acids in the intestine. Niu et al found that mulberry leaves can increase the content of short‐chain fatty acids, especially acetic acid, by affecting intestinal flora, thereby affecting lipid deposition (Niu et al, 2023). Related studies have found that mulberry leaf water extract can reduce the serum free fatty acid level in hyperglycemic mice (Han et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive lipidomic analysis revealed the effects of fermented Morus alba L. intake on lipid profile in backfat and muscle tissue of Yuxi black pigs

Hou,

Ji,

Chu

et al. 2024

Animal Physiology Nutrition

View full text Add to dashboard Cite

Mulberry leaf is a widely used protein feed and is often used as a strategy to reduce feed costs and improve meat quality in the livestock industry. However, to date, there is a lack of research on the improvement of meat quality using mulberry leaves, and the exact mechanisms are not yet known. The results showed that fermented mulberry leaves significantly reduced backfat content but had no significant effect on intramuscular fat (IMF). Lipidomic analysis showed that 98 and 303 differential lipid molecules (p < 0.05) were identified in adipose and muscle tissues, respectively, including triglycerides (TG), phosphatidylcholine, phosphatidylethanolamine, sphingolipids, and especially TG; therefore, we analysed the acyl carbon atom number of TG. The statistical results of acyl with different carbon atom numbers of TG in adipose tissue showed that the acyl group containing 13 carbon atoms (C13) in TG was significantly upregulated, whereas C15, C16, C17, and C23 were significantly downregulated, whereas in muscle tissue, the C12, C19, C23, C25, and C26 in TG were significantly downregulated. Acyl changes in TG were different for different numbers of carbon atoms in different tissues. We found that the correlations of C (14–18) in adipose tissue were higher, but in muscle tissue, the correlations of C (18–26) were higher. Through pathway enrichment analysis, we identified six and four metabolic pathways with the highest contributions of differential lipid metabolites in adipose and muscle tissues respectively. These findings suggest that fermented mulberry leaves improve meat quality mainly by inhibiting TG deposition by downregulating medium‐ and short‐chain fatty acids in backfat tissue and long‐chain fatty acids in muscle tissue.

show abstract

“…Conversely, the relative abundance of norank_f_norank_o_Oscillospirales was notably lower in JZ compared to JC (p < 0.05). Notably, Terrisporobacter and Cellulosilyticum have been documented as effective cellulose degraders, contributing to enhanced feed digestion Cai and Dong, 2010;Niu et al, 2023).…”

Section: Effects Of Yps Addition On Donkey Gut Bacteriamentioning

confidence: 99%

Yeast polysaccharide supplementation: impact on lactation, growth, immunity, and gut microbiota in Dezhou donkeys

Huang,

Khan,

Chen

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionThe Dezhou donkey, a prominent Chinese breed, is known for its remarkable size, rapid growth, and resilience to tough feeding conditions, and disease resistance. These traits are crucial in meeting the growing demand for Ejiao and donkey meat. Yeast polysaccharide (YPS), a functional polysaccharide complex known for its immune-enhancing and growth-promoting properties in livestock and poultry, remains relatively understudied in donkeys.ObjectivesThis study aimed to investigate the impact of YPS supplementation on lactating and growing Dezhou donkey jennies and foals.Materials and methodsTwelve 45-day-old Dezhou donkey foals and their jennies, matched for body weight and age, were randomly allocated to two dietary groups: a control group receiving a basal diet and an experimental group receiving the basal diet supplemented with 10 g/pen of YPS. The experiment was conducted over a 23-day period, during which donkey foals and lactating jennies were co-housed.Results and discussionThe findings revealed that YPS supplementation had no adverse effects on milk production or composition in Dezhou donkey jennies but significantly increased feed intake. Additionally, YPS was associated with increased plasma glucose and creatinine concentrations in foals, while tending to decrease alkaline phosphatase, white blood cell count, red blood cell count, and hemoglobin levels (p < 0.10). Immune indices demonstrated that YPS supplementation elevated the levels of immunoglobulin A (IgA) and immunoglobulin G (IgG) in jennies (p < 0.05) and increased complement component C4 concentrations in foals (p < 0.05). Moreover, YPS positively influenced the fecal microbiome, promoting the abundance of beneficial microorganisms such as Lactobacillus and Prevotella in donkey foals and Terriporobacter and Cellulosilyticum in jennies, all of which contribute to enhanced feed digestion. Additionally, YPS induced alterations in the plasma metabolome for both jennies and foals, with a predominant presence of lipids and lipid-like molecules. Notably, YPS increased the concentrations of specific lipid metabolites, including 13,14-Dihydro PGF2a, 2-Isopropylmalic acid, 2,3-Dinor-TXB2, Triterpenoids, Taurocholic acid, and 3b-Allotetrahydrocortisol, all of which are associated with improved animal growth.ConclusionIn conclusion, this study suggests that dietary supplementation of YPS enhances feed intake, boosts immunity by increasing immunoglobulin levels, stimulates the growth-promoting gut microbiota (Lactobacillus and Prevotella), and exerts no adverse effects on the metabolism of both Dezhou donkey jennies and foals.

show abstract

Effects of Silage Diet on Meat Quality through Shaping Gut Microbiota in Finishing Pigs

Abstract: To avoid competition between people and animals for food, it is essential to develop nontraditional feeds. In this study, the effects of the silages of the unconventional feed resources mulberry and paper mulberry on meat quality of finishing pigs were examined.

Cited by 20 publications

References 58 publications

Vitamin K2 supplementation improves impaired glycemic homeostasis and insulin sensitivity for type 2 diabetes through gut microbiome and fecal metabolites

Vitamin K2 supplementation improves impaired glycemic homeostasis and insulin sensitivity for type 2 diabetes through gut microbiome and fecal metabolites

Comprehensive lipidomic analysis revealed the effects of fermented Morus alba L. intake on lipid profile in backfat and muscle tissue of Yuxi black pigs

Yeast polysaccharide supplementation: impact on lactation, growth, immunity, and gut microbiota in Dezhou donkeys

Contact Info

Product

Resources

About