Whole-body vibration improves the anti-inflammatory status in elderly subjects through toll-like receptor 2 and 4 signaling pathways

Rodriguez‐Miguelez, Paula; Fernandez‐Gonzalo, Rodrigo; Collado, Pilar S.; Almar, Mar; Martínez‐Flórez, Susana; Paz, José Antonio de; González‐Gallego, Javier; Cuevas, María J.

doi:10.1016/j.mad.2015.08.002

Cited by 45 publications

(57 citation statements)

References 51 publications

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“…MT biomarkers measured in the circulation include lipopolysaccharide binding protein 1 (LBP1) and the co-receptor soluble CD14, both involved in the presentation of antigens to toll like receptors present on most cell membranes. MT has been observed in healthy older people who were overweight and associates with higher inflammatory biomarkers (10–12). Recent data demonstrates that intestinal barrier dysfunction correlates with lifespan across a range of Drosophila genotypes and environmental conditions (13).…”

Section: Introductionmentioning

confidence: 99%

Reduced intestinal motility, mucosal barrier function, and inflammation in aged monkeys

Mitchell

Davis

Brass

et al. 2017

The Journal of nutrition, health and aging

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Objective: We aimed to examine the general health and intestinal physiology of young and old non-human primates with comparable life histories and dietary environments. Design: Vervet monkeys (Chlorcebus aethiops sabaeus) in stable and comparable social and nutritional environments were selected for evaluation. Health phenotype, circulating cytokines and biomarkers of microbial translocation (MT) were measured (n=26–44). Subsets of monkeys additionally had their intestinal motility, intestinal permeability, and fecal microbiomes characterized. These outcomes document age-related intestinal changes present in the absence of nutritional stressors, which are all known to affect gastrointestinal motility, microbiome, and MT. Results: We found that old monkeys have greater systemic inflammation and poor intestinal barrier function as compared to young monkeys. Old monkeys have dramatically reduced intestinal motility, and all changes in motility and MT are present without large differences in fecal microbiomes. Conclusion: We conclude that deteriorating intestinal function is a feature of normal aging and could represent the source of inflammatory burden yet to be explained by disease or diet in normal aging human primate populations. Intestinal changes were seen independent of dietary influences and aging within a consistent environment appears to avoid major microbiome shifts. Our data suggests interventions to promote intestinal motility and mucosal barrier function have the potential to support better health with aging.

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Section: Introductionmentioning

confidence: 99%

Reduced intestinal motility, mucosal barrier function, and inflammation in aged monkeys

Mitchell

Davis

Brass

et al. 2017

The Journal of nutrition, health and aging

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“…Indeed, cross-sectional studies have confirmed an association between physical inactivity and moderate degree of inflammation in healthy individuals [13] and in the elderly population [14]. In addition, regular physical activity induces an attenuation of biomarkers of inflammation and toll-like receptor (TLR) signalling pathways in old subjects, suggesting that physical exercise could suppress moderate systemic inflammation in the elderly [15, 16]. However, other specialized innate immune receptors, such as the NOD-like receptor family, pyrin domain containing 3 (NLRP3), seem to mediate chronic low-level inflammation during aging and in aging-related pathologies [17].…”

Section: Introductionmentioning

confidence: 99%

Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects

Mejías-Peña

Estébanez

Rodriguez‐Miguelez

et al. 2017

Aging

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Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NLRP3 inflammasome, and apoptosis in peripheral blood mononuclear cells (PBMCs) from old subjects. Twenty-six healthy women and men (age, 69.6±1.5 yr) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Protein expression of beclin-1, Atg12, Atg16 and LAMP-2 increased following the training program, while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser757 were significantly lower. Resistance exercise also induced a decrease in NLRP3 expression and in the caspase-1/procaspase-1 ratio. Expression of Bcl-2 and Bcl-xL, as well as the Bad/BcL-2 ratio were reduced, and there was a significant decrease in the protein content of caspase-3. The results obtained seem to indicate that 8-week resistance training stimulates autophagy, prevents NLRP3 inflammasome activation, and reduces apoptosis in PBMCs from elderly subjects. These data could have a significant impact in prevention and rehabilitation programs currently employed in elderly population.

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“…Upon stimulation, these receptors lead to the recruitment of different signaling adaptors such as myeloid differentiation primary response gene 88 (MyD88) and TIR domain-containing adaptor-inducing interferon (TRIF). Both processes result in the activation of a number of downstream signaling pathways, which control inflammatory and immune responses (Rodriguez-Miguelez et al 2015). It has been described that several autophagy-related proteins have an inhibitory effect on TLR signaling, and it is known that p62/SQSTM1 is involved in the suppression of TLR4-induced activation of mitogen-activated protein kinases .…”

Section: Introductionmentioning

confidence: 99%

Effects of aerobic training on markers of autophagy in the elderly

Mejías-Peña

Rodriguez‐Miguelez

Fernandez‐Gonzalo

et al. 2016

AGE

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Autophagy is a molecular process essential for the maintenance of cellular homeostasis, which appears to (i) decline with age and (ii) respond to physical exercise. In addition, recent evidence suggests a crosstalk between autophagy and toll-like receptor (TLR)-associated inflammatory responses. This study assessed the effects of aerobic exercise training on autophagy and TLR signaling in older subjects. Twentynine healthy women and men (age, 69.7 ± 1.0 year) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week aerobic training program, while CG followed their daily routines. Peripheral blood mononuclear cells were isolated from blood samples obtained before and after the intervention, and protein levels of protein 1 light chain 3 (LC3), sequestosome 1 (p62/SQSTM1), beclin-1, phosphorylated unc-51-like kinase (ULK-1), ubiquitin-like autophagy-related (Atg)12, Atg16, and lysosome-associated membrane protein (LAMP)-2 were measured. TLR2 and TLR4 signaling pathways were also analyzed. Peak oxygen uptake increased in TG after the intervention. Protein expression of beclin-1, Atg12, Atg16, and the LC3II/I ratio increased following the training program (p < 0.05), while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser 757 were lower (p < 0.05). Protein content of TLR2, TLR4, myeloid differentiation primary response gen 88 (MyD88), and TIR domaincontaining adaptor-inducing interferon (TRIF) were not significantly modified by exercise. The current data indicate that aerobic exercise training induces alterations in multiple markers of autophagy, which seem to be unrelated to changes in TLR2 and TLR4 signaling pathways. These results expand knowledge on exerciseinduced autophagy adaptations in humans and suggest that the exercise type employed may be a key factor explaining the potential relationship between autophagy and TLR pathways.

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Whole-body vibration improves the anti-inflammatory status in elderly subjects through toll-like receptor 2 and 4 signaling pathways

Cited by 45 publications

References 51 publications

Reduced intestinal motility, mucosal barrier function, and inflammation in aged monkeys

Reduced intestinal motility, mucosal barrier function, and inflammation in aged monkeys

Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects

Effects of aerobic training on markers of autophagy in the elderly

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