Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria

Lin, Yongxin; Ding, Weixin; Liu, Deyan; He, Tiehu; Yoo, Gayoung; Yuan, Junji; Chen, Zengming; Fan, Jianling

doi:10.1016/j.soilbio.2017.06.001

Cited by 168 publications

(37 citation statements)

References 59 publications

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“…In general, higher soil pHs lead to increased nitrification (Xiao, Xu, Tang, Zhang, & Brookes, ), higher amoA gene abundance and release more N 2 O. For example, straw biochar stimulated N 2 O emissions by enhancing the amoA genes of ammonia‐oxidizing bacteria in the nitrification process, which was significantly related to soil pH (Lin et al, ). But the decreased amoA gene abundance and increased soil pH in our study (Figure and Figure S4) indicated that the soil pH increased by manure‐based biochar was not the dominant factor affecting soil N 2 O emission in nitrification.…”

Section: Discussionmentioning

confidence: 99%

“…EMC-affected redox properties of manure-based biochars are the important factors mediating soil N 2 O emission and microbial N cycling genes in a low-quality red soil. This adds to our current understanding that biochar pH (Lin et al, 2017), N availability , porosity (Quin et al, 2014), adsorption capacity (Quin et al, 2015), and toxic organic compounds (Clough, Condron, Kammann, & Müller, 2013) in lignocellulose-based biochars play important roles in regulating N 2 O emissions and microbial N cycling genes. A summary diagram that highlights the mechanisms of manure biochar regulated N 2 O emission and N cycling microbial community is presented in Figure 8 .…”

Section: Conclusion and Implicationmentioning

confidence: 91%

“…In general, changes in the N cycling genes occur following the addition of lignocellulose‐based biochar. For instance, wheat straw‐derived biochar increased the abundance of bacterial amoA genes but did not alter the abundance of nirK, nirS, and nosZ genes (Lin et al, ). Biochars derived from green waste or rice straw increased the abundance of nosZ gene, leading to a reduction of N 2 O emission, especially in high‐moisture soils (Feng, Sheng, Cai, Wang, & Zhu, ; Harter et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes

Dai

Zhang

et al. 2018

Land Degrad Dev

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Manure‐based biochar is an effective amendment to increase both carbon sequestration and the fertility of degraded soils, whereas the responses of N2O emissions and microbial‐N cycling genes to its application and the underlying mechanisms are poorly understood. Here, four biochars were produced under two pyrolysis temperatures (300 and 700°C) and with two organic C extraction procedures (water and acetone extraction). The resulting biochars were either with relative enrichment or depletion in easily mineralizable carbon (EMC) compared with recalcitrant C. We added biochars, with urea and sodium nitrate, to a degraded red soil and incubated the amended soils at moisture levels of 60% and 130% field capacity. All the biochars decreased nitrification gene abundance, that is, amoA. Biochars with EMC had greater stimulatory effects on the abundance of denitrification genes (nirK, nirS, and nosZ) and N2O emission, regardless of moisture level and N form, compared with biochar without EMC. Biochar‐induced microbial activity, biochar aliphatics, and alkyl groups correlated positively with N2O emission and denitrification gene abundance. The water dissolved organic C of biochar facilitated the conversion of N2O to N2, whereas the acetone extractable C fraction postponed the completion of denitrification. In conclusion, the N2O emission and denitrification gene abundance increased with decreasing biochar aromaticity and increasing EMC content. Our study emphasizes that the EMC supply in manure‐based biochars also importantly mediates soil N2O emission and microbial N cycling genes, adding to current understanding of influencing factors such as biochar pH, porosity, N availability, and redox property.

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Section: Discussionmentioning

confidence: 99%

Section: Conclusion and Implicationmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes

Dai

Zhang

et al. 2018

Land Degrad Dev

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“…High-throughput sequencing and bioinformatics analysis. We used primer sets Arch-amoA26F/ Arch-amoA417R (62) and amoA-1F/amoA-2R (63) for AOA and AOB amoA gene amplification, respectively, where the PCR program and reaction composition of AOA and AOB were described previously by Park et al (62) and Lin et al (64), respectively. A unique sample-identifying barcode was added to the forward primer in PCR amplification, triplicate PCR amplifications were conducted and pooled for each sample, and PCR products were subsequently purified by using a Qiagen gel extraction kit.…”

Section: Methodsmentioning

confidence: 99%

Nitrosospira Cluster 8a Plays a Predominant Role in the Nitrification Process of a Subtropical Ultisol under Long-Term Inorganic and Organic Fertilization

Lin

Luo

et al. 2018

Appl Environ Microbiol

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Long-term effects of inorganic and organic fertilization on nitrification activity (NA) and the abundances and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated in an acidic Ultisol. Seven treatments applied annually for 27 years comprised no fertilization (control), inorganic NPK fertilizer (N), inorganic NPK fertilizer plus lime (CaCO) (NL), inorganic NPK fertilizer plus peanut straw (NPS), inorganic NPK fertilizer plus rice straw (NRS), inorganic NPK fertilizer plus radish (NR), and inorganic NPK fertilizer plus pig manure (NPM). In nonfertilized soil, the abundance of AOA was 1 order of magnitude higher than that of AOB. Fertilization reduced the abundance of AOA but increased that of AOB, especially in the NL treatment. The AOA communities in the control and the N treatments were dominated by the and B1 clades but shifted to clade A in the NL and NPM treatments. cluster 8a was found to be the most dominant AOB in all treatments. NA was primarily regulated by soil properties, especially soil pH, and the interaction with AOB abundance explained up to 73% of the variance in NA. When NL soils with neutral pH were excluded from the analysis, AOB abundance, especially the relative abundance of cluster 8a, was positively associated with NA. In contrast, there was no association between AOA abundance and NA. Overall, our data suggest that cluster 8a of AOB played an important role in the nitrification process in acidic soil following long-term inorganic and organic fertilization. The nitrification process is an important step in the nitrogen (N) cycle, affecting N availability and N losses to the wider environment. Ammonia oxidation, which is the first and rate-limiting step of nitrification, was widely accepted to be mainly regulated by AOA in acidic soils. However, in this study, nitrification activity was correlated with the abundance of AOB rather than that of AOA in acidic Ultisols. cluster 8a, a phylotype of AOB which preferred warm temperatures, and low soil pH played a predominant role in the nitrification process in the test Ultisols. Our results also showed that long-term application of lime or pig manure rather than plant residues altered the community structure of AOA and AOB. Taken together, our findings contribute new knowledge to the understanding of the nitrification process and ammonia oxidizers in subtropical acidic Ultisol under long-term inorganic and organic fertilization.

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“…Chen et al (2010) [23] showed that the abundance and composition of AOA and AOB are affected by different soil types. The study on rice and wheat rotation from Jiangsu Province of China demonstrated that the application of wheat straw biochar increased the number of bacterial amoA genes [37]. Moreover, Ke et al (2013) [36] demonstrated that soil compartments could be the most important factor that differentiate the nitrifying community activity and composition.…”

Section: Other Factorsmentioning

confidence: 99%

Ammonia Oxidizing Archaea and Bacteria in East Asian Paddy Soils—A Mini Review

2017

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Ammonia oxidation is crucial in nitrogen removal and global nitrogen dynamics since it is the first step of the nitrification process. In this review, we focus on the distribution and community structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in East Asian paddy soils with variable soil properties. The available East Asian paddy soil data shows that the ammonium concentration and pH ranges from 0.4 to 370 mg/kg and 5.1 to 8.2, respectively. Our meta-analysis suggest that AOA specific gene sequences are generally more abundant than those of AOB in both acidic and alkaline paddy soils, where Nitrosophaera and Nitrosospira amoA clusters mainly dominate the microbial community, respectively. In addition, the contribution of ammonia oxidizers to the nitrification process has been demonstrated using DNA-SIP (DNA-based stable-isotope probing); the results of these studies indicate that pH is the most important factor in niche separation of AOA and AOB under a variety of edaphic conditions. Finally, we discuss a number of other environmental variables that affect the abundance, distribution, and activity of AOA and AOB in East Asian paddy soils.

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Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria

Cited by 168 publications

References 59 publications

Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes

Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes

Nitrosospira Cluster 8a Plays a Predominant Role in the Nitrification Process of a Subtropical Ultisol under Long-Term Inorganic and Organic Fertilization

Ammonia Oxidizing Archaea and Bacteria in East Asian Paddy Soils—A Mini Review

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Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria

Cited by 168 publications

References 59 publications

Easily mineralizable carbon in manure‐based biochar added to a soil influences N2O emissions and microbial‐N cycling genes

Easily mineralizable carbon in manure‐based biochar added to a soil influences N2O emissions and microbial‐N cycling genes

Nitrosospira Cluster 8a Plays a Predominant Role in the Nitrification Process of a Subtropical Ultisol under Long-Term Inorganic and Organic Fertilization

Ammonia Oxidizing Archaea and Bacteria in East Asian Paddy Soils—A Mini Review

Contact Info

Product

Resources

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Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes

Easily mineralizable carbon in manure‐based biochar added to a soil influences N₂O emissions and microbial‐N cycling genes