Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Abstract

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

“…These alterations stimulate the structural and functional changes such as degradation of extracellular matrix (ECM) proteins and the upregulation of MMPs, thereby promoting skin photoaging [27]. In most previous studies, UVB irradiation onto the skin of hailress mice or keratinocytes was widely used to evaluate the therapeutic effects of natural products with anti-photogaing activity [28][29][30]. However, UVA and UVB expose induced a similar response on the MMP regulation of HDF cells although UVA radiation can be considered more suitable to study dermal fibroblast responses [28].…”

Effect of Rice (Oryza sativa L.) Ceramides Supplementation on Improving Skin Barrier Functions and Depigmentation: An Open-Label Prospective Study

“…An increase in sebum production leads to the augmentation of skin softness and improves skin elasticity. These improvements are likely caused by protective effects of skin surface lipid layer against UVB-induced skin damage, thereby improving skin hydration, and appearance of wrinkle [15]. Ceramides administration was also proven to be effective in downregulating the enzymatic activity of matrix metalloproteinase-1 (MMP-1) and MMP-13, a collagenase family, while simultaneously improving the biosynthesis of procollagen type I [15,45,46].…”

“…These improvements are likely caused by protective effects of skin surface lipid layer against UVB-induced skin damage, thereby improving skin hydration, and appearance of wrinkle [15]. Ceramides administration was also proven to be effective in downregulating the enzymatic activity of matrix metalloproteinase-1 (MMP-1) and MMP-13, a collagenase family, while simultaneously improving the biosynthesis of procollagen type I [15,45,46]. The suppression of MMPs is likely due to the upregulation of tissue inhibitors for metalloproteinases (TIMPs) in the presence of high ceramides [46].…”

“…Glucosylceramides, a glycoside of ceramides, are a major sphingo (glycol) lipid in plants. The beneficial effects of glucosylceramides for improving skin hydration and restoring skin barrier function have been established for plant sources such as konjac [13], beet [14], and wheat [15]. Although foregoing evidence supported beneficial effects of topical ceramides for restoration of the skin barrier, studies on oral ceramides are extremely scarce, with most the published data being derived from in vivo and in vitro models.…”

Boosting the Photoaged Skin: The Potential Role of Dietary Components

“…Wheat extract oil supplementation improved UVB-induced losses in skin moisture, elasticity, and damage of skin barrier function, and inhibited the decreases in procollagen type I, HA, and ceramide, in mouse dorsal skin tissue [ 120 ].…”