What is your diagnosis? Liver from a cow

Emanuelli, Mauren Picada; Antoniazzi, Alfredo Q.; Cecim, Marcelo; Fighera, Rafael A.

doi:10.1111/vcp.12414

Cited by 3 publications

(3 citation statements)

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“…For this reason, we used a limit of GGT concentration to categorize the cows in the present study into two groups, as the blood concentration of GGT is an indicative of liver damage, subsequently confirmed by cytological and histological analyses. The use of cytology as a fast-diagnostic method (Emanuelli et al 2016) and the histology as the gold standard (Barros et al 2007) to diagnose Senecio spp. poisoning confirmed the detection of seneciosis in all the cows in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…For cytological analysis, slides were prepared with each liver fragment using the imprint technique, air-dried and stained with Diff-Quik ® (Diff-Quik ® Differential Stain Set, American Scientific Products). Cytological evaluation was based on the typical histological findings associated to pyrrolizidine alkaloid toxicosis (megalocytosis, biliary hyperplasia and fibrosis), as described (Emanuelli et al 2016). Markedly enlarged hepatocytes (megalocytes) defined megalocytosis; biliary hyperplasia was determined based on the amounts of biliary epithelial cells; fibrosis was determined based on the amounts of fibroblasts.…”

Section: Rna Extraction Reverse Transcription and Real-time Pcrmentioning

confidence: 99%

“…in reproductive tissues, there is one study reporting a sporadic effect on cells expressing P-450 cytochrome, such as large steroidogenic cells in the corpus luteum (CL) (Krabbe et al 2015). Seneciosis diagnosis is determined by the presence of the plant, epidemiological aspects and assessment of liver function (Karam et al 2004), characterized by megalocytosis, fibrosis, and hyperplasia of biliary ducts on histopathological exam as the gold standard (Barros et al 2007), but also cytological exam can be used (Emanuelli et al 2016). Another additional approach is the biochemical diagnosis of Senecio spp.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of interferon-stimulated gene 15 (ISG15) expression in blood neutrophils in beef cattle poisoned by Senecio spp.

et al. 2021

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This study aimed to assess liver damage and interferon-stimulated gene 15 (ISG15) blood expression as a consequence of embryonic signaling on maternal recognition of pregnancy in beef cattle presenting natural ingestion of Senecio spp. Epidemiological aspects, as the presence of the plant, associated to gamma glutamyl transferase (GGT) activity can be used as Senecio spp. poisoning diagnosis. Maternal recognition of pregnancy period occurs when the embryo secretes interferon tau (IFNT) to signal its presence to the mother and eventually extend corpus luteum (CL) lifespan. In our study, liver damage was determined by concentration serum GGT, cytological and histopathological examinations. Reproductive status was evaluated by concentration of progesterone, CL diameter and ISG15 mRNA expression on Day 19 following fixed-time artificial insemination (FTAI). Cows were categorized into two groups based on concentration of GGT: Group 1 (GGT<30U/L) and 2 (GGT>31U/L). No difference on body condition scores was observed. All the cows presented liver damage based on cytology and histopathological exams. Cows from the Group 1 had higher pregnancy rate, presenting larger CL diameter and greater concentration of progesterone. Interestingly, ISG15 mRNA expression had no difference between Groups 1 and 2, even presenting difference in pregnancy status. These findings suggest embryonic loss beyond Day 19. It suggests late embryonic mortality may be associated to liver insufficiency. In conclusion, liver injury and/or concentration of GGT does not alter ISG15 expression on blood neutrophils, however cows presenting lower concentration of GGT (<30U/L) had increased pregnancy status. Therefore, the concentration of GGT allow us to screen liver status and foresee a successful pregnancy in beef cattle.

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Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction Reverse Transcription and Real-time Pcrmentioning

confidence: 99%