What is BCG?

Grange, J. M.; Gibson, Julia; Osborn, T.W.; Collins, C.H.; Yates, M.D.

doi:10.1016/0041-3879(83)90038-7

Cited by 61 publications

(33 citation statements)

References 20 publications

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“…Since 1921, the year the BCG vaccine was put into use in humans, there has been widespread use of the vaccine by the majority of countries in the world with the exception of The Netherlands and the United States, countries where immunization has traditionally been restricted to specific indications (3)(4)(5)(6).…”

Section: Discussionmentioning

confidence: 99%

A Complication of BCG Vaccine: A Case of Localized Cutaneous Abscess due to Mycobacterium bovis

Lussier

Bourgault

Gaudreau

et al. 1998

Canadian Journal of Infectious Diseases and Medical Microbiolog

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The attenuated bacille Calmette-Guérin (BCG) vaccine is administered to prevent tuberculosis. Complications of vaccination are uncommon. A case of cutaneous abscess due to BCG is presented in a 24-year-old woman. The abscess developed at the inoculation site four weeks after vaccination. Routine Gram stain and bacterial cultures of the pus were negative. The auramine stain was positive. Mycobacterial cultures were positive after 14 and 18 days, using the BACTEC 12B bottle and Löwenstein-Jensen media, respectively. The mycobacteria were identified asMycobacterium bovis, vaccinal strain by high-performance liquid chromatography and DNA probe assays.

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Section: Discussionmentioning

confidence: 99%

A Complication of BCG Vaccine: A Case of Localized Cutaneous Abscess due to Mycobacterium bovis

Lussier

Bourgault

Gaudreau

et al. 1998

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Although bacterial numbers in the livers of SCID mice infected with the trpD mutant were maintained at approximately log 10 5, in both the spleen and lung there was a rapid decrease in organ load postinfection to around or below the limits of detection by plating. This was maintained until the termination of the experiment at day 90.…”

Section: Growth Kinetics Of H37rv Versus Mutant M Tuberculosis Straimentioning

confidence: 99%

“…Thus, whereas avirulent BCG was generated as a result of serial passage, a new live, attenuated vaccine can now be generated in a rational manner (10). The complete genome sequence of M. tuberculosis reveals numerous potential new drug and vaccine targets for investigation (3).…”

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confidence: 99%

Characterization of Auxotrophic Mutants of Mycobacterium tuberculosis and Their Potential as Vaccine Candidates

et al. 2001

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Auxotrophic mutants of Mycobacterium tuberculosis have been proposed as new vaccine candidates. We have analyzed the virulence and vaccine potential of M. tuberculosis strains containing defined mutations in genes involved in methionine (metB), proline (proC), or tryptophan (trpD) amino acid biosynthesis. The metB mutant was a prototrophic strain, whereas the proC and trpD mutants were auxotrophic for proline and tryptophan, respectively. Following infection of murine bone marrow-derived macrophages, H37Rv and the metB mutant strain survived intracellularly for over 10 days, whereas over 90% of proC and trpD mutants were killed during this time. In SCID mice, both H37Rv and the metB mutant were highly virulent, with mouse median survival times (MST) of 28.5 and 42 days, respectively. The proC mutant was significantly attenuated (MST, 130 days), whereas the trpD mutant was essentially avirulent in an immunocompromised host. Following infection of immunocompetent DBA mice with H37Rv, mice survived for a median of 83.5 days and the metB mutant now showed a clear reduction in virulence, with two of five infected mice surviving for 360 days. Both proC and trpD mutants were avirulent (MST of >360 days). In vaccination studies, prior infection with either the proC or trpD mutant gave protection equivalent (proC mutant) to or better (trpD mutant) than BCG against challenge with M. tuberculosis H37Rv. In summary, proC and trpD genes are essential for the virulence of M. tuberculosis, and mutants with disruptions in either of these genes show strong potential as vaccine candidates.

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“…The current tuberculosis vaccine, the Bacille Calmette-Guerin (BCG), was derived from an isolated Mycobacterium bovis that had been attenuated in the early 19 th century (7,21). Because the BCG vaccine is derived from live bacteria, potency tests of the vaccine usually rely on the viable count assay for batch control purposes (6,15).…”

Section: Introductionmentioning

confidence: 99%