Wettability studies of topologically distinct titanium surfaces

Kulkarni, M.S.; Patil-Sen, Yogita; Junkar, Ita; Kulkarni, Chandrashekhar V.; Lorenzetti, Martina; Iglič, Aleš

doi:10.1016/j.colsurfb.2015.03.024

Cited by 112 publications

(80 citation statements)

References 48 publications

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“…However, in the high-resolution images, particle-like oxide appeared on the ridges and valleys of the pits and holes structure, which was rather smooth in the control group. With a prolonged oxidation time, the particle-like oxide grew sturdier from approximately an average of 20 nm in the TO-2 group to an average of 60 nm in the TO-6 group, which has been shown to influence surface wettability32.…”

Section: Discussionmentioning

confidence: 99%

Surface thermal oxidation on titanium implants to enhance osteogenic activity and in vivo osseointegration

Wang

et al. 2016

Sci Rep

125

113

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Thermal oxidation, which serves as a low-cost, effective and relatively simple/facile method, was used to modify a micro-structured titanium surface in ambient atmosphere at 450 °C for different time periods to improve in vitro and in vivo bioactivity. The surface morphology, crystallinity of the surface layers, chemical composition and chemical states were evaluated by field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Cell behaviours including cell adhesion, attachment, proliferation, and osteogenic differentiation were observed in vitro study. The ability of the titanium surface to promote osseointegration was evaluated in an in vivo animal model. Surface thermal oxidation on titanium implants maintained the microstructure and, thus, both slightly changed the nanoscale structure of titanium and enhanced the crystallinity of the titanium surface layer. Cells cultured on the three oxidized titanium surfaces grew well and exhibited better osteogenic activity than did the control samples. The in vivo bone-implant contact also showed enhanced osseointegration after several hours of oxidization. This heat-treated titanium enhanced the osteogenic differentiation activity of rBMMSCs and improved osseointegration in vivo, suggesting that surface thermal oxidation could potentially be used in clinical applications to improve bone-implant integration.

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Section: Discussionmentioning

confidence: 99%

Surface thermal oxidation on titanium implants to enhance osteogenic activity and in vivo osseointegration

Wang

et al. 2016

Sci Rep

125

113

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“…Even though the used electrophoretic mobility technique allowed the determination of ζ of the NTs in suspension, no significant difference was observed between the NTs with different diameters, either in terms of isoelectric point (IEP) values or ζ magnitude in dependence of the pH. Therefore, in the next step, the zeta potential of self-assembled TiO 2 NTs on Ti surfaces was measured by streaming potential technique, when still adhered to the substrate [16]. Streaming potential represents a reliable method to evaluate the surface charge by determining the zeta potential ( ζ ) at the interface between the material and the fluid around [12, 13, 16].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, in the next step, the zeta potential of self-assembled TiO 2 NTs on Ti surfaces was measured by streaming potential technique, when still adhered to the substrate [16]. Streaming potential represents a reliable method to evaluate the surface charge by determining the zeta potential ( ζ ) at the interface between the material and the fluid around [12, 13, 16]. Differently from electrophoresis and electroacoustic techniques, the streaming potential technique consists in the formation of an electric field as the electrolyte flows tangentially to a stationary charged solid surface, so that the zeta potential is calculated out of the generated streaming potential.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic Properties of TiO2 Nanotubular Surfaces

et al. 2016

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Surface charge is one of the most significant properties for the characterisation of a biomaterial, being a key parameter in the interaction of the body implant with the surrounding living tissues. The present study concerns the systematic assessment of the surface charge of electrochemically anodized TiO2 nanotubular surfaces, proposed as coating material for Ti body implants. Biologically relevant electrolytes (NaCl, PBS, cell medium) were chosen to simulate the physiological conditions. The measurements were accomplished as titration curves at low electrolytic concentration (10−3 M) and as single points at fixed pH but at various electrolytic concentrations (up to 0.1 M). The results showed that all the surfaces were negatively charged at physiological pH. However, the zeta potential values were dependent on the electrolytic conditions (electrolyte ion concentration, multivalence of the electrolyte ions, etc.) and on the surface characteristics (nanotubes top diameter, average porosity, exposed surface area, wettability, affinity to specific ions, etc.). Accordingly, various explanations were proposed to support the different experimental data among the surfaces. Theoretical model of electric double layer which takes into account the asymmetric finite size of ions in electrolyte and orientational ordering of water dipoles was modified according to our specific system in order to interpret the experimental data. Experimental results were in agreement with the theoretical predictions. Overall, our results contribute to enrich the state-of-art on the characterisation of nanostructured implant surfaces at the bio-interface, especially in case of topographically porous and rough surfaces.

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“…One of the important issues can be the contact area between the buffer/water on TiO 2 NTs, which changes in regard to TiO 2 NT diameter, 89 as well as wettability of the NTs. More hydrophilic NTs enable a better connection with buffer/water, which is reflected also in the pocket effect and better protein binding on the 100 nm diameter NTs.…”

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confidence: 99%

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Kulkarni

Flašker

Lokar

et al. 2015

IJN

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Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO 2 ) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices.

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Wettability studies of topologically distinct titanium surfaces

Cited by 112 publications

References 48 publications

Surface thermal oxidation on titanium implants to enhance osteogenic activity and in vivo osseointegration

Surface thermal oxidation on titanium implants to enhance osteogenic activity and in vivo osseointegration

Electrokinetic Properties of TiO2 Nanotubular Surfaces

Binding of plasma proteins to titanium dioxide nanotubes with different diameters

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