Weissella cibaria MG5285 and Lactobacillus reuteri MG5149 attenuated fat accumulation in adipose and hepatic steatosis in high-fat diet-induced C57BL/6J obese mice

Abstract

Background: Excessive consumption of dietary fat is closely related to obesity, diabetes, insulin resistance, cardiovascular disease, hypertension, and non-alcoholic fatty liver disease. Recently, probiotics have been highly proposed as biotherapeutic to treat and prevent diseases. Previously, there are studies that demonstrated the beneficial effects of probiotics against metabolic disorders, including obesity and diabetes.
 Objective: We investigated the anti-obesity effect and mechanism of action of four human-derived lactic acid bacterial (LAB) strains (Lacticaseibacillus rhamnosus MG4502, Lactobacillus gasseri MG4524, Limosilactobacillus reuteri MG5149, and Weissella cibaria MG5285) in high-fat diet (HFD)-induced obese mice.
 Design: Obesity was induced in mice over 8 weeks, with a 60% HFD. The four human-derived LAB strains (2 × 108 CFU/mouse) were orally administered to male C57BL/6J mice once daily for 8 weeks. Body weight, liver and adipose tissue (AT) weights, glucose tolerance, and serum biochemistry profiles were determined. After collecting the tissues, histopathological and Western blot analyses were conducted.
 Results: Administration of these LAB strains resulted in decreased body weight, liver and AT weights, and glucose tolerance. Serum biochemistry profiles, including triglyceride (TG), total cholesterol, low-density lipoprotein cholesterol, and leptin, pro-inflammatory cytokines, improved. Hepatic steatosis and TG levels in liver tissue were significantly reduced. In addition, the size of adipocytes in epididymal tissue was significantly reduced. In epididymal tissues, Limosilactobacillus reuteri MG5149 and Weissella cibaria MG5285 groups showed a significantly reduced expression of lipogenic proteins, including peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, fatty acid synthase (FAS), and adipocyte-protein 2. In addition, sterol regulatory element-binding protein 1-c and its downstream protein FAS in the liver tissue were significantly decreased. These strains attenuated fat accumulation in the liver and AT by upregulating the phosphorylation of AMP-activated protein kinase and acetyl-CoA carboxylase in HFD-fed mice.
 Conclusion: We suggest that L. reuteri MG5149 and W. cibaria MG5285 could be used as potential probiotic candidates to prevent obesity.

Limosilactobacillus reuteri in Health and Disease

“…On the other hand, according to another study, the L. reuteri MG5149 strain reduced body weight, adipose tissue weight, and adipocyte size in epididymal tissue. In addition, it inhibited the expression of lipogenic proteins such as peroxisome proliferator-activated receptor, CCAAT/enhancer-binding protein, fatty acid synthase, and adipocyte-protein 2 in some tissues and reduced fat accumulation by upregulating the phosphorylation of AMP-activated protein kinase and acetyl-CoA carboxylase [ 26 ]. Furthermore, treatment with L. reuteri JBD30l lowered the concentration of free fatty acids (FFAs) in the small intestine’s gut fluid, lowering intestinal FFA absorption while increasing fecal FFA excretion [ 27 ].…”

Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review

“…Studies in mice and rats have shown, among other things, a positive effect of probiotic preparations on glucose balance [ 78 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 112 , 113 , 118 ] and reduced insulin resistance [ 78 , 87 , 89 , 90 , 91 , 92 , 94 , 113 ]. Andersson et al [ 97 ], Sakai et al [ 98 ], Park et al [ 114 ], Naito et al [ 99 ], Wang et al [ 100 , 101 ], Machado et al [ 102 ], Lee et al [ 103 ], Hsu et al [ 115 ], Kim et al [ 116 ] and Zeng et al [ 104 ] have shown that the probiotic addition of different species of the genus Lactobacillus or a combination of probiotics [ 100 , 101 , 116 ] improves glucose sensitivity (tolerance) [ 97 , 98 , 99 , 100 , 101 , 102 , 103 , 114 , 115 , 116 ] and reduces insulin resistance [ 97 , 98 , 99 , …”

Polygonatum kingianum Polysaccharides Enhance the Preventive Efficacy of Heat-Inactivated Limosilactobacillus reuteri WX-94 against High-Fat-High-Sucrose-Induced Liver Injury and Gut Dysbacteriosis

“…The heat-inactivated probiotics containing dead cells and their metabolites can exert health-promoting biological responses, restoring the normal intestinal homeostasis, in many cases similar or even superior to that seen with live cells. ,− Although mechanisms underlying the beneficial effects of heat-inactivated probiotics remain inconclusive, the consistently observed benefits of inactivated probiotics may be attributed to the microbial metabolites. ,,, Here, we found that adding PKP in the culture medium successfully augmented protective effects of pasteurized L. reuteri against HFHS-induced liver injury, impaired hepatic lipid metabolism, and gut dysbacteriosis, which were superior to PKP per se and the cosupplementation of live L.…”